期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 28, 期 2, 页码 207-226出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2016.1262164
关键词
Foaming; TIPS; tissue engineering; scaffolds; chitosan; PVA
资金
- University of Melbourne, Melbourne Materials Institute
- Bio21 Institute of Molecular Science and Biotechnology (Bio21)
In this paper, a novel foaming methodology consisting of turbulent mixing and thermally induced phase separation (TIPS) was used to generate scaffolds for tissue engineering. Air bubbles were mechanically introduced into a chitosan solution which forms the continuous polymer/liquid phase in the foam created. The air bubbles entrained in the foam act as a template for the macroporous architecture of the final scaffolds. Wet foams were crosslinked via glutaraldehyde and frozen at -20 degrees C to induce TIPS in order to limit film drainage, bubble coalescence and Ostwald ripening. The effects of production parameters, including mixing speed, surfactant concentration and chitosan concentration, on foaming are explored. Using this method, hydrogel scaffolds were successfully produced with up to 80% porosity, average pore sizes of 120m and readily tuneable compressive modulus in the range of 2.6 to 25kPa relevant to soft tissue engineering applications. These scaffolds supported 3T3 fibroblast cell proliferation and penetration and therefore show significant potential for application in soft tissue engineering.
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