Article
Plant Sciences
Henrique Uliana Trentin, Grigorii Batiru, Ursula Karoline Frei, Somak Dutta, Thomas Lubberstedt
Summary: The doubled haploid technology is a feasible and cost-efficient way to produce completely homozygous lines in maize, with factors such as haploid induction rate, donor background, and environmental conditions contributing to its success. Different genetic backgrounds showed varied haploid induction rates in different environments, with poor-performing donors possibly affected by anthocyanin inhibitor genes.
Article
Agronomy
Jiaojiao Ren, Xiaoyu Zhang, Zongze Li, Penghao Wu
Summary: This study used an F-2 population derived from inbred maize lines to dissect the genetic basis of maternal haploid inducibility (MHI) through QTL analysis and QTL by environment interaction analysis. Ten QTLs for MHI were identified, with three candidate genes involved in seed development. The study provides valuable information for understanding the genetic basis of MHI in maize breeding.
Article
Plant Sciences
S. Wang, K. Ouyang
Summary: Haploid induction is a technique that can speed up the breeding process by creating true-breeding lines in a single generation. Scientists have recently developed a new method using a cytosine base editor (CBE) to generate point mutations in CENH3, which induces haploids. This innovative technology provides a new perspective for haploid induction in crops and offers various options for breeders.
Article
Plant Sciences
Dmitri Demidov, Inna Lermontova, Michael Moebes, Andriy Kochevenko, Joerg Fuchs, Oda Weiss, Twan Rutten, Eberhard Sorge, Erika Zuljan, Ricardo Fabiano Hettwer Giehl, Martin Mascher, Saravanakumar Somasundaram, Udo Conrad, Andreas Houben
Summary: Targeted degradation of CENH3 protein can be used to generate haploids in Arabidopsis thaliana. By using a recombinant anti-GFP nanobody fused to ligase proteins, EYFP-tagged CENH3 can be recognized and degraded through the ubiquitin-proteasome pathway. This strategy achieves a haploid frequency of up to 7.6%.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Henrique Uliana Trentin, Matheus Dalsente Krause, Rajkumar Uttamrao Zunjare, Vinicius Costa Almeida, Edicarlos Peterlini, Valeriy Rotarenco, Ursula Karoline Frei, William Dale Beavis, Thomas Lubberstedt
Summary: By conducting a genome-wide association study on a panel of 159 maternal haploid inducers, we identified multiple genes associated with haploid induction in maize. One major gene near MTL was found to be present in all inducers and necessary for disrupting haploid induction. Additionally, a significant locus on chromosome 10 was identified, which could be used in marker-assisted selection approaches for inducer breeding programs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Na Wang, Jonathan Gent, R. Kelly Dawe
Summary: Maize lines heterozygous for cenh3 null mutation can be crossed with wild-type plants to produce haploid progeny, making the method simpler and more versatile for various applications.
Review
Horticulture
Anjali Rai, Kavita Dubey, Sung Soo Han
Summary: The discovery of a specific histone variant that can induce haploid development has provided a powerful technique for breeding new crop varieties. Recent advancements in this technology have shown that non-transgenic changes to the histone variant can also induce haploids. This review paper summarizes the progress made in using this technique to develop haploid plant crops and provides insights into the detailed mechanism of haploid induction.
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2023)
Article
Agronomy
Chenxu Liu, Jinlong Li, Ming Chen, Wei Li, Yu Zhong, Xin Dong, Xiaowei Xu, Chen Chen, Xiaolong Tian, Shaojiang Chen
Summary: Doubled haploid (DH) technology plays a crucial role in modern maize breeding, and the efficiency of haploid induction and identification is determined by haploid inducers. In this study, we developed a new high-oil haploid inducer line, CHOI4, with improved haploid induction rate (HIR) and identification accuracy by using molecular marker-assisted selection and the number of haploids per ear as phenotypic criterion. CHOI4 shows great potential in enhancing the efficiency of large-scale DH breeding programs with reduced costs compared to previous inducer lines.
Article
Agronomy
Dexuan Meng, Chenxu Liu, Shaojiang Chen, Weiwei Jin
Summary: The application of maize hybrids has significantly improved yields, and the double haploid breeding technology accelerates the breeding process. Maize DH breeding is playing an increasingly important role in commercial breeding.
MOLECULAR BREEDING
(2021)
Article
Biochemistry & Molecular Biology
Chenglin Jiang, Ju Sun, Rui Li, Shijuan Yan, Wei Chen, Liang Guo, Guochen Qin, Pengcheng Wang, Cheng Luo, Wenjie Huang, Qinghua Zhang, Alisdair R. Fernie, David Jackson, Xiang Li, Jianbing Yan
Summary: This study investigated the molecular mechanism of haploid induction (HI) in maize and discovered a new gene controlling HI. The findings suggest that the balance of reactive oxygen species (ROS) plays a critical role in maintaining normal reproduction and provide a potential route to accelerate crop breeding.
Article
Biochemistry & Molecular Biology
Ulkar Ahmadli, Manikandan Kalidass, Lucie Crhak Khaitova, Joerg Fuchs, Maria Cuacos, Dmitri Demidov, Sheng Zuo, Jana Pecinkova, Martin Mascher, Mathieu Ingouff, Stefan Heckmann, Andreas Houben, Karel Riha, Inna Lermontova
Summary: Double haploid production is a highly effective method to establish true-breeding lines in a single generation. This study focuses on haploid induction in Arabidopsis and identifies a mutant of the cenH3 assembly factor KNL2 as a potential haploid inducer. The efficiency of haploid induction was increased by 10-fold when the mutant was exposed to short-term temperature stress. Furthermore, a point mutation in the KNL2 gene was found to be sufficient to generate haploid-inducing lines. This research provides insights into the factors affecting haploid induction and offers new possibilities for the development of haploid-inducing lines in crops.
PLANT COMMUNICATIONS
(2023)
Article
Plant Sciences
Harjot Kaur, Mohammed Kyum, Surinder Sandhu, Gagandeep Singh, Priti Sharma
Summary: The optimized protocol using CIMMYT haploid inducer CIM2GTAIL P2 significantly improves the haploid induction rate and survival rate in sub-tropical maize, accelerating the hybrid breeding program and reducing the production cost of doubled haploid.
Article
Agronomy
Mohd Kyum, Harjot Kaur, Akashdeep Kamboj, Lakshay Goyal, Dharminder Bhatia
Summary: Rice, a model crop and staple food crop, holds a prominent position in plant sciences. Doubled haploid (DH) breeding in rice offers advantages over conventional methods in accelerating pure lines development. Recent research focuses on key molecular factors behind haploid induction (HI) trait in rice, providing potential for rice improvement programs.
Article
Plant Sciences
Wei Zhang, Yongting Ma, Zhendong Zhu, Liangjun Huang, Asif Ali, Xuan Luo, Ying Zhou, Yun Li, Peizhou Xu, Jin Yang, Zhuang Li, Haoran Shi, Jisheng Wang, Wanzhuo Gong, Qiong Zou, Lanrong Tao, Zeming Kang, Rong Tang, Zhangjie Zhao, Zhi Li, Shixing Guo, Shaohong Fu
Summary: This study compares different cytoplasmic genotypes to investigate the mechanism of double haploid induction in rapeseed. The induction effect is influenced by the interaction between maternal karyogene and cytoplasmic genotype, possibly involving selective elimination of paternal chromosomes.
Article
Plant Sciences
Henrique Uliana Trentin, Recep Yavuz, Abil Dermail, Ursula Karoline Frei, Somak Dutta, Thomas Lubberstedt
Summary: The effectiveness of haploid induction systems depends on high haploid induction rate (HIR) and resource savings. An isolation field approach for hybrid induction is proposed. The success of efficient haploid production relies on inducer traits such as high HIR, abundant pollen production, and tall plants. Evaluation of seven hybrid inducers and their respective parents showed that mid-parent heterosis benefited plant height, ear height, and tassel size. Two promising hybrid inducers, BH201/LH82-Ped126 and BH201/LH82-Ped128, were identified for haploid induction in isolation fields. Hybrid inducers offer convenience and resource-effectiveness by improving plant vigor without compromising HIR.