Article
Multidisciplinary Sciences
Masahiko Terajima, Yuki Taga, Tomoyuki Nakamura, Hou-Fu Guo, Yukako Kayashima, Nobuyo Maeda-Smithies, Kshitij Parag-Sharma, Jeong Seon Kim, Antonio L. Amelio, Kazunori Mizuno, Jonathan M. Kurie, Mitsuo Yamauchi
Summary: This study investigated the role of LH2 in collagen stability, organization, and mineralization using LH2-null MC3T3 cells. The results demonstrated that LH2 is involved in the hydroxylation of telopeptidyl Lys residues of collagen and affects collagen solubility, fibril diameter, and mineralization.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Matthew E. Cockman, Yoichiro Sugimoto, Hamish B. Pegg, Norma Masson, Eidarus Salah, Anthony Tumber, Helen R. Flynn, Joanna M. Kirkpatrick, Christopher J. Schofield, Peter J. Ratcliffe
Summary: JMJD6 is a 2-oxoglutarate-dependent dioxygenase involved in various cellular processes. Through mass spectrometry analysis, we identified 150 lysine hydroxylation sites on 48 protein substrates catalyzed by JMJD6, including 19 hydroxylation sites on BRD4. Most of these substrates are associated with membraneless organelle formation, suggesting a potential role for JMJD6 in regulating subcellular partitioning in response to stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Daria Chirita, Pauline Bronnec, Flora Magnotti, Sarah Dalmon, Amandine Martin, Michel Popoff, Mathieu Gerfaud-Valentin, Pascal Seve, Alexandre Belot, Anne Contis, Agnes Duquesne, Gaetane Nocturne, Irene Lemelle, Sophie Georgin-Lavialle, Guilaine Boursier, Isabelle Touitou, Yvan Jamilloux, Thomas Henry
Summary: This study reveals the role of the B30.2 domain and the central helical scaffold (CHS) domain in regulating pyrin inflammasome activation in Familial Mediterranean Fever (FMF). Mutations in these domains can have different functional impacts on the pyrin inflammasome, contributing to the diversity of pyrin-associated autoinflammatory diseases.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Masahiko Terajima, Yuki Taga, Becky K. Brisson, Amy C. Durham, Kotaro Sato, Katsuhiro Uzawa, Tomoaki Saito, Shunji Hattori, Karin U. Sorenmo, Mitsuo Yamauchi, Susan W. Volk
Summary: The study characterized the molecular and morphological phenotypes of type I collagen in canine mammary carcinoma, finding longer and wider collagen fibers as well as an increase in stable collagen cross-links in neoplastic samples. Mass spectrometric analyses revealed over-hydroxylation of lysine residues in malignant mammary tumor samples, potentially promoting tumor cell metastasis. Furthermore, expression levels of genes encoding lysyl hydroxylase 2 (LH2) and its molecular chaperone FK506-binding protein 65 were significantly increased in neoplastic samples, suggesting a role in LH2-mediated stable collagen cross-links in promoting tumor cell metastasis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Keith Zientek, Nobuyo Mizuno, Antti M. Salo, Olesya Semenova, Sara F. Tufa, Douglas R. Keene, Paul Holden, Kazunori Mizuno, Douglas B. Gould, Johanna Myllyharju, Hans Peter Bachinger
Summary: Collagen is the most abundant protein in humans, with a complex biosynthesis involving multiple enzymes and chaperones. LH1 and P3H3 play distinct but related roles in lysyl hydroxylation of collagen, with LH1 having a global enzymatic role and P3H3 being involved particularly at cross-link formation sites.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Physiology
M. Wolak, J. Drobnik, E. Bojanowska
Summary: Research indicates that the effects of exendin-4 on the production of extracellular matrix and regulatory proteins in human skin fibroblasts vary depending on exposure to normal or high glucose levels. While exendin-4 has beneficial effects on fibroblasts under normoglycemic conditions, these positive effects are attenuated under hyperglycemic conditions.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Morten M. Jensen, Arkadiusz Bonna, Sigurd J. Frederiksen, Samir W. Hamaia, Peter Hojrup, Richard W. Farndale, Henrik Karring
Summary: This study identifies the presence of Dermatopontin (DPT) in porcine corneal stroma and explores its binding sites on collagens II and III. The binding sites for DPT on triple-helical collagens are found to be located at the crosslinking and collagenase cleavage sites. The results suggest that disrupted DPT binding to fibrillar collagens may contribute to certain connective tissue disorders. These findings provide a foundation for further investigation of the role of DPT.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2022)
Article
Chemistry, Analytical
Yuki Taga, Masashi Kusubata, Kazunori Mizuno
ANALYTICAL CHEMISTRY
(2020)
Article
Medicine, Research & Experimental
Xiaochao Tan, Lei Shi, Priyam Banerjee, Xin Liu, Hou-Fu Guo, Jiang Yu, Neus Bota-Rabassedas, B. Leticia Rodriguez, Don L. Gibbons, William K. Russell, Chad J. Creighton, Jonathan M. Kurie
Summary: Loss of TP53 initiates a pharmacologically actionable secretory process in lung adenocarcinoma (LUAD) cells. Activation of G55-dependent secretion promotes proliferation and invasion of TP53-deficient LUAD cells. Targeting the G55-G45 interaction with a small molecule impairs secretion and reduces growth and metastasis of TP53-deficient LUAD.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Keith Zientek, Nobuyo Mizuno, Antti M. Salo, Olesya Semenova, Sara F. Tufa, Douglas R. Keene, Paul Holden, Kazunori Mizuno, Douglas B. Gould, Johanna Myllyharju, Hans Peter Bachinger
Summary: Collagen is the most abundant protein in humans, with a complex biosynthesis involving multiple enzymes and chaperones. LH1 and P3H3 play distinct but related roles in lysyl hydroxylation of collagen, with LH1 having a global enzymatic role and P3H3 being involved particularly at cross-link formation sites.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Sport Sciences
Eri Miyamoto-mikami, Hiroshi Kumagai, Kumpei Tanisawa, Yuki Taga, Kosuke Hirata, Naoki Kikuchi, Nobuhiro Kamiya, Ryoko Kawakami, Taishi Midorikawa, Takuji Kawamura, Ryo Kakigi, Toshiharu Natsume, Hirofumi Zempo, Koya Suzuki, Yoshimitsu Kohmura, Kazunori Mizuno, Suguru Torii, Shizuo Sakamoto, Koichiro Oka, Mitsuru Higuchi, Hisashi Naito, Naokazu Miyamoto, Noriyuki Fuku
Summary: The study investigated the association between the COL1A1 rs1107946 variant and fatigue fracture/muscle injury in athletes, and found that C-allele carriers had a higher risk of fatigue fracture but a lower risk of muscle injury. In nonathletes, C-allele carriers showed lower BMD and muscle stiffness.
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
(2021)
Article
Multidisciplinary Sciences
Masahiko Terajima, Yuki Taga, Becky K. Brisson, Amy C. Durham, Kotaro Sato, Katsuhiro Uzawa, Tomoaki Saito, Shunji Hattori, Karin U. Sorenmo, Mitsuo Yamauchi, Susan W. Volk
Summary: The study characterized the molecular and morphological phenotypes of type I collagen in canine mammary carcinoma, finding longer and wider collagen fibers as well as an increase in stable collagen cross-links in neoplastic samples. Mass spectrometric analyses revealed over-hydroxylation of lysine residues in malignant mammary tumor samples, potentially promoting tumor cell metastasis. Furthermore, expression levels of genes encoding lysyl hydroxylase 2 (LH2) and its molecular chaperone FK506-binding protein 65 were significantly increased in neoplastic samples, suggesting a role in LH2-mediated stable collagen cross-links in promoting tumor cell metastasis.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Hou-Fu Guo, Neus Bota-Rabassedas, Masahiko Terajima, B. Leticia Rodriguez, Don L. Gibbons, Yulong Chen, Priyam Banerjee, Chi-Lin Tsai, Xiaochao Tan, Xin Liu, Jiang Yu, Michal Tokmina-Roszyk, Roma Stawikowska, Gregg B. Fields, Mitchell D. Miller, Xiaoyan Wang, Juhoon Lee, Kevin N. Dalby, Chad J. Creighton, George N. Phillips, John A. Tainer, Mitsuo Yamauchi, Jonathan M. Kurie
Summary: Guo et al. have determined the molecular basis of collagen lysyl hydroxylase 2 (LH2) substrate specificity, showing that LH2 functions not only as a telopeptidyl lysyl hydroxylase, but also as a collagen glucosyltransferase to drive lung cancer progression. The research suggests that LH2 plays a crucial role in promoting LUAD progression through a variety of mechanisms, including tLH-dependent and tLH-independent pathways.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kentaro Ide, Sanai Takahashi, Keiko Sakai, Yuki Taga, Tomonori Ueno, David Dickens, Rosalind Jenkins, Francesco Falciani, Takako Sasaki, Kazuhiro Ooi, Shuichi Kawashiri, Kazunori Mizuno, Shunji Hattori, Takao Sakai
Summary: The dipeptide Pro-Hyp has pronounced effects on mouse tendon cells, promoting differentiation and maturation, increased chemotactic activity, and enhanced cell proliferation. Proteomic analysis predicted potential pathways affected by Pro-Hyp, while mechanistically, cells treated with Pro-Hyp showed increased directional persistence and migration velocity. These findings suggest the potential application of Pro-Hyp in translational medicine.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Tomoaki Saito, Masahiko Terajima, Yuki Taga, Fumihiko Hayashi, Sachi Oshima, Atsushi Kasamatsu, Yasuhiko Okubo, Chizuru Ito, Kiyotaka Toshimori, Masataka Sunohara, Hideki Tanzawa, Katsuhiro Uzawa, Mitsuo Yamauchi
Summary: This study demonstrates that significantly diminished levels of LH2 in LH2(+/-) mice result in decreased hydroxylation of lysine residues and collagen cross-linking in collagen, leading to reduced mineral densities and compromised mechanical properties in bones. These findings highlight the important role of LH2 in collagen molecular phenotype, bone matrix mineralization, and mechanical properties.
Article
Biochemistry & Molecular Biology
Kazunori K. Fujii, Yuki Taga, Yusuke K. Takagi, Ryo Masuda, Shunji Hattori, Takaki Koide
Summary: Procollagen forms a triple helix structure in the endoplasmic reticulum with post-translational modifications enhancing its thermal stability. Collagen properties remain constant at lower temperatures, but at higher temperatures, an increase in post-translational modifications leads to higher thermal stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Shunji Hattori, Tomomi Kiriyama-Tanaka, Masashi Kusubata, Yuki Taga, Testuya Ebihara, Yuki Kumazawa, Katsuyuki Imai, Mitsutaka Miura, Yoshihiro Mezaki, Alexei Tikhonov, Haruki Senoo
Summary: The investigation revealed that the extracellular matrix in the soft tissues of 40,000-year-old frozen mammoths was well-preserved, mainly consisting of collagen protein. The study showed that the triple helical collagen molecule has been resistant to proteinase digestion and preserved in the soft tissues of these frozen mammoths.
Article
Multidisciplinary Sciences
Masahiko Terajima, Yuki Taga, Tomoyuki Nakamura, Hou-Fu Guo, Yukako Kayashima, Nobuyo Maeda-Smithies, Kshitij Parag-Sharma, Jeong Seon Kim, Antonio L. Amelio, Kazunori Mizuno, Jonathan M. Kurie, Mitsuo Yamauchi
Summary: This study investigated the role of LH2 in collagen stability, organization, and mineralization using LH2-null MC3T3 cells. The results demonstrated that LH2 is involved in the hydroxylation of telopeptidyl Lys residues of collagen and affects collagen solubility, fibril diameter, and mineralization.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Thibault Coste, Sara F. Tufa, Douglas R. Keene, Kazunori Mizuno, Elisabeth Tournier-Lasserve, Douglas B. Gould
Summary: Collagens are important proteins in the body, and mutations in genes encoding them can cause connective tissue disorders. Lysyl hydroxylase 3 (LH3) plays a critical role in collagen biosynthesis, and LH3 pathogenic variants may contribute to Gould syndrome.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Yuki Taga, Yu Iwasaki, Chisa Tometsuka, Noriko Funato, Yasutaka Shigemura, Masashi Kusubata, Kazunori Mizuno
Summary: Studies have found that Gly-3Hyp-4Hyp tripeptide can be detected in human blood at high concentrations after ingestion of collagen hydrolysate, and it exhibits stability and resistance to degradation enzymes. This tripeptide has potential for health promotion and disease treatment.
NPJ SCIENCE OF FOOD
(2022)
Article
Biochemistry & Molecular Biology
Shinji Takeyari, Takuo Kubota, Yasuhisa Ohata, Makoto Fujiwara, Taichi Kitaoka, Yuki Taga, Kazunori Mizuno, Keiichi Ozono
Summary: Osteogenesis imperfecta is a hereditary disease caused by mutations in two type I collagen genes. Treatment with 4-phenylbutyric acid may normalize collagen synthesis, reduce ER retention, and improve misfolding of collagen helix in the extracellular matrix.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Chisa Tometsuka, Noriko Funato, Kazunori Mizuno, Yuki Taga
Summary: The study discovered that GDCH has anti-obesity effects in mice, significantly reducing the levels of triglycerides and cholesterol in the blood, while inhibiting the expression of genes related to fat synthesis. However, single oral administration of GDCH did not lower blood lipid levels in ICR mice. These results suggest that the lipid-lowering effects of GDCH are achieved through long-term regulation of lipid metabolism.
CURRENT RESEARCH IN FOOD SCIENCE
(2021)