Article
Chemistry, Analytical
Xiaohui Wang, Wenyu Jin, Yao Yang, Huizi Ma, Honghong Liu, Jiawen Lei, Yuhua Wu, Li Zhang
Summary: We propose a CRISPR/Cas12a Mediated Enzymatic Recombinase Amplification detection system (CAMERA) for the detection of adulteration in pork ingredients in halal food. CAMERA combines Enzymatic Recombinase Amplification (ERA) and Cas12a cleavage to achieve high specificity and sensitivity. It can detect samples with as few as two copies of porcine-specific gene and quantify samples with as low as 5% porcine DNA content within 25 minutes. The system does not require technical expertise or professional equipment, making it a valuable tool for qualitative and quantitative detection in resource-limited areas.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biology
Yihong He, Wenxian Chen, Jindai Fan, Shuangqi Fan, Hongxing Ding, Jinding Chen, Lin Yi
Summary: Porcine parvovirus (PPV) infection is a major concern in the swine industry globally, and it is crucial to establish a rapid and efficient detection method. A new recombinase-aided amplification (RAA) assay was developed in this study, which showed high specificity for PPV detection, increased sensitivity compared to PCR, and cost-saving benefits.
Article
Biochemical Research Methods
Wuyin Zhang, Liang Xu, Qi Liu, Yingli Cao, Kankan Yang, Xiangjun Song, Ying Shao, Jian Tu, Kezong Qi
Summary: Porcine circovirus type 3 (PCV3) is a disease causing significant economic losses in swine herds, but the ERA-CRISPR/Cas12a technology can rapidly, simply, and sensitively detect PCV3 with good specificity, demonstrating single-molecule level detection capability.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Biochemical Research Methods
Siyuan Liu, Dagang Tao, Yuying Liao, Yalan Yang, Shouzhang Sun, Yulan Zhao, Peng Yang, Yijie Tang, Bin Chen, Yonggang Liu, Shengsong Xie, Zhonglin Tang
Summary: This study developed a new method for highly sensitive and visual detection of PRRSV within 25 minutes by integrating the RT-RPA-Cas12a system with a single stranded DNA-fluorescently quenched reporter. The technique showed single copy sensitivity and no cross-reactivity with other porcine viruses. The results were highly accurate when compared with quantitative RT-qPCR, making it a promising tool for on-site detection of PRRSV.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Plant Sciences
Kihye Shin, Soon-Hwa Kwon, Seong-Chan Lee, Young-Eel Moon
Summary: This study established a RPA-CRISPR/Cas12a-LFA system for rapid diagnosis of citrus scab, which is highly sensitive and does not cross-react with other fungal diseases.
Article
Food Science & Technology
Liyun Lin, Guangcai Zha, Huagui Wei, Yuzhong Zheng, Peikui Yang, Yaqun Liu, Mouquan Liu, Zhonghe Wang, Xianghui Zou, Hui Zhu, Qiulan Luo, JinQuan Li, Min Lin
Summary: The study developed a detection method for Staphylococcus aureus in food using a combination of recombinase polymerase amplification (RPA) and CRISPR-Cas12a technology. The method demonstrated high sensitivity in detecting low quantities of S. aureus and proved feasible for use in both laboratory and field samples. This accurate and portable detection method has great potential in food safety applications.
Article
Forestry
Jing Zhou, Hanqian Dai, Tingting Dai, Tingli Liu
Summary: This study established a detection method for Phytophthora cambivora using a combination of whole-genome screening, recombinase polymerase amplification (RPA), and CRISPR/Cas12a. The RPA-CRISPR/Cas12a assay showed specific detection of P. cambivora isolates and could detect the presence of P. cambivora in apple fruits. Compared to conventional techniques, this method is faster, more cost-effective, and suitable for on-site detection at entry ports.
Article
Immunology
Shuang Liu, Siyuan Huang, Fang Li, Yuanyuan Sun, Jin Fu, Fei Xiao, Nan Jia, Xiaolan Huang, Chunrong Sun, Juan Zhou, Yi Wang, Dong Qu
Summary: The study developed a simple, rapid, sensitive, and specific detection platform for P. aeruginosa infection. The newly developed P. aeruginosa-CRISPR-RPA assay showed high sensitivity and specificity, and could complete P. aeruginosa detection within half an hour.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Feina Li, Jing Xiao, Haiming Yang, Yao Yao, Jieqiong Li, Huiwen Zheng, Qian Guo, Xiaotong Wang, Yuying Chen, Yajie Guo, Yonghong Wang, Chen Shen
Summary: In this study, a rapid and efficient method utilizing RPA-CRISPR/Cas12a technology was proposed for the diagnosis of Mycoplasma pneumoniae. This method eliminates the need for expensive instruments and specialized personnel. The entire process, including sample extraction, RPA reaction, CRISPR/Cas12a detection, and visual observation, can be completed within 1 hour with high sensitivity and specificity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Endocrinology & Metabolism
Juan Dong, Shanshan Cheng, Xiaoqun Yang, Bing Liao, Zhen Ruan, Shuangqing Zhang, Fukang Luo
Summary: This study developed a rapid detection method for Mycoplasma pneumoniae (MP) adhesion protein P1 by merging recombinase polymerase amplification (RPA) and CRISPR/Cas12a gene editing. The method is fast and convenient, ensuring the specificity, rapid efficiency, and visualization of the test results without relying on instruments and professional personnel. It provides a solution to the subjective judgment of the culture test method and the difficulty of smear preparation.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Chemistry, Analytical
Yiran Xiao, Honglin Ren, Han Wang, Deying Zou, Yixin Liu, Haosong Li, Pan Hu, Yansong Li, Zengshan Liu, Shiying Lu
Summary: A low-cost platform based on the CRISPR/Cas12a system was designed for the rapid detection of Listeria monocytogenes. This method combines CRISPR/Cas12a and recombinase polymerase amplification (RPA) to provide a contamination-free platform for nucleic acid detection with high specificity and ultrasensitivity. The optimized method can specifically detect target DNA in 25 minutes and successfully detect LM in spiked pork samples and natural meat samples.
Article
Immunology
Hong Lin, Song Zhao, Yanhong Liu, Lei Shao, Yuying Ye, Nizhen Jiang, Kun Yang
Summary: The newly designed RAA-LFD detection method is suitable for reliably detecting Plasmodium species in low-resource environments with limited laboratory capabilities. The method is performed in an incubator at 37 degrees Celsius for 15 minutes, with visible amplicons on flow dipsticks within 3 minutes. Sensitivity for a single recombinant plasmid was 1 copy per microliter, with sensitivities for genomic DNA from patients infected with P. falciparum, P. vivax, P. ovale, and P. malariae ranging from 0.1pg/ul to 100pg/ul. Overall sensitivity was 100pg/ul.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ke Yao, Deliang Peng, Chen Jiang, Wei Zhao, Guangkuo Li, Wenkun Huang, Lingan Kong, Haifeng Gao, Jingwu Zheng, Huan Peng
Summary: The study developed a species-specific RPA primer for detecting H. schachtii, which showed higher sensitivity and faster results compared to traditional PCR methods. Sugar beet nematodes were successfully detected in field samples using RPA, demonstrating 100% accuracy in the existing samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Yuting Xiao, Dongliang Fei, Ming Li, Yueyu Ma, Mingxiao Ma
Summary: In this study, a visual detection assay for DWV-A and DWV-B was established using RPA-CRISPR-Cas12a-LFD technology. The method showed high specificity and sensitivity, with results obtained in a short period of time. Validation with traditional methods demonstrated the accuracy of this detection assay for DWV infection.
Article
Chemistry, Analytical
Xingzhi Wu, Dan Jin, Wei Ni, Feng Wang, Yuling Zhang, Guo-Jun Zhang
Summary: Rapid and accurate identification of Methicillin-resistant Staphylococcus aureus (MRSA) is crucial to prevent the spread of infections and guide effective treatment. In this study, a rapid, specific, and ultrasensitive colorimetric assay based on recombinase polymerase amplification (RPA) and magnetic nanoparticles (MNPs) assisted CRISPR/Cas12a was developed for the detection of the mecA gene in MRSA bacteria. This method allows for the rapid and accurate identification of MRSA within 2 hours through the observable color change.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Nanoscience & Nanotechnology
Yanan Li, Xingyu Chen, Li Yu, Dan Pang, Hongjie Yan, Meijie Chen
Summary: Visibly transparent radiative cooling (VTRC) has great potential in energy-saving buildings or car glasses. Balancing lighting and cooling performance is crucial to VTRC. A Janus VTRC coating consisting of a thermal emitter, PDMS, and a transparent near-infrared reflector, TiO2/Ag/TiO2, was designed. This technology can save 34-44% of the annual cooling energy consumption.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yue Zhou, Yanmin Wang, Deqiang Kong, Qianqian Zhao, Lei Zhao, Jialin Zhang, Xuemin Chen, Yanan Li, Yan Xu, Chao Meng
Summary: The construction of bifunctional catalysts with balanced adsorption towards multiple reactants and intermediates is crucial for efficient urea-assisted water splitting. In this study, a dual-center catalytic mechanism is proposed for urea electrolysis, where NiS with introduced Mo4+ sites exhibits excellent UOR/HER bifunctionality. The crumpled flower-like nanostructure provides abundant active sites and rapid mass transfer, leading to energy savings and high current density in the urea-assisted water electrolyzer.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Polymer Science
Yanan Li, Wenfang Zheng, Wenxi Li, Renming Pan, Xiangyang Lin
Summary: This review focuses on the internal plasticization of energetic polymers from different dimensions, aiming to provide inspiration for designing and optimizing mechanical properties as well as further research.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Green & Sustainable Science & Technology
Zijian Jiang, Jia Wei, Yifei Zhang, Xiruo Niu, Jiamei Li, Yanan Li, Guoping Pan, Mengdie Xu, Xueru Cui, Nan Cui, Jun Li
Summary: To address water pollution, the development of efficient and environmental-friendly catalysts is crucial. In this study, a nitrogen-enriched biochar encapsulated cobalt nanoparticles composite (Co@N-BC) was synthesized and used for efficient persulfate activation, resulting in high removal of DOX in a short time. Electron transfer was identified as the primary pathway for DOX elimination, and the Co@N-BC/PS system showed excellent stability and resistance to interference.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Multidisciplinary
Yongkang Wu, Xiaoke Li, Kang Hua, Xiao Duan, Rui Ding, Zhiyan Rui, Feng Cao, Mengchen Yuan, Jia Li, Jianguo Liu
Summary: The development of efficient platinum group metal-free catalysts is crucial for the widespread application of proton exchange membrane fuel cells. However, the performance and lifespan of nitrogen-coordinated atomic iron intercalated in carbon matrix (Fe-N-C) catalysts are still obstacles. In this study, a highly-active and stable Fe-N-C catalyst is developed using a controllable N-C coating strategy. The stability enhancement is achieved through reduced carbon corrosion and iron loss. These findings contribute to the development of Fe-N-C catalysts with high activity and long lifetimes.
Article
Chemistry, Multidisciplinary
Hong Wen, Liqi Li, Xinyue Ge, Yang Fei, Yanan Li, Menghuan Li, Weidong Xiao, Zhong Luo
Summary: A multifunctional biomimetic hydrogel, resembling natural blood coagulation mechanism, is developed to treat ulcerative colitis (UC). The hydrogel acts as an artificial colonic mucosa, forming a bioadhesive barrier at the ulcer site and restoring immune and microbial homeostasis. This hydrogel presents a promising approach for effective UC therapy.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Environmental
Qiuyun Lu, Lingling Yang, Pamela Chelme-Ayala, Yanan Li, Xuehua Zhang, Mohamed Gamal El-Din
Summary: In this study, the capability of surface microlenses (MLs) as a clean technology for more efficient photocatalytic water decontamination was demonstrated. By controlling the conditions of light treatment, MLs could enhance the photocatalytic degradation efficiency (eta) by up to 402%. Optical simulations and experimental results showed that MLs optimize the light distribution and promote the formation of active species, leading to the enhancement of eta. The use of MLs may serve as a novel strategy to improve the photocatalytic degradation of micropollutants, especially in places with weak light sources.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Yanan Li, Dadi Xie, Luxi Li, Pei Jiang
Summary: This study aimed to explore the metabolic changes induced by hypertension at different ages. The results showed that spontaneously hypertensive rats (SHR) at 5 weeks old and 32 weeks old had different metabolic characteristics, mainly involving amino acid metabolism, glycolysis, lipid metabolism, and amino acid metabolism.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2023)
Article
Engineering, Environmental
Guoping Pan, Jia Wei, Mengdie Xu, Jiamei Li, Linhao Wang, Yanan Li, Nan Cui, Jun Li, Zhaoxu Wang
Summary: In recent years, metal-free catalysts have been widely used to remove contaminants in water. This study synthesized boron-doped biochars through a simple pyrolysis approach and found that they exhibited excellent capability to activate persulfate for efficient oxytetracycline degradation. The study also investigated the influence of the-O-B-O-groups on the biochars on the degradation rate and found that they enhanced the electron-donating capacity of the biochars. Additionally, the degradation process of oxytetracycline was pH-dependent and the degradation pathways and toxicity changes during the process were determined.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Jingya Hua, Yang Cui, Lili Guo, Hongyan Li, Jie Fan, Yanan Li, Yonghong Wang, Kankan Liu, Qiusheng He, Xinming Wang
Summary: Formaldehyde (HCHO) concentrations were measured at different sites in Taiyuan, and the results showed that HCHO plays a significant role in atmospheric ozone formation. The sources of HCHO varied among different locations, with secondary formation being the dominant source at some sites and solvent usage and coking sources being the major contributors at others.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Physical
Di Zhang, Bo Niu, Yanan Li, Zhaojin Li, Huan Wang, Qiujun Wang, Fei Yuan, Zhilin Hu, Bo Wang
Summary: Graphene materials have been found to be effective anodes for potassium-ion batteries due to their safety, low cost, and environmental friendliness. However, the repeated insertion/extraction of large-sized K-ions into/from the electrode at low voltage regions often leads to volume expansion and poor cycle stability. To address this issue, researchers have synthesized S-doped hard carbon material (NSHCM2) using biomass and thiourea, which has been found to expand the distance between carbon layers and create more defects, resulting in improved structure stability and reversible capacity. The optimized NSHCM2 electrode exhibits a high capacity of 294 mAh g-1 at 200 mA g-1 and excellent cycling stability of 220 mAh g-1 at 2000 mA g-1 over 5000 cycles. This simple and effective synthesis route enables durable and fast potassium storage.
SOLID STATE IONICS
(2023)
Article
Agronomy
Dongdong Li, Zikun Zhu, Xiaomin Deng, Jianan Zou, Chao Ma, Candong Li, Tao Yin, Chunyan Liu, Jinhui Wang, Qingshan Chen, Dawei Xin
Summary: Soybean is a protein and oil-rich legume crop that relies on symbiotic nitrogen fixation for growth. This study investigated the interaction between NopT and NopP in soybean nodulation and identified five candidate genes involved in their crosstalk pathway using RNA-seq analysis. GmPBS1 was found to be the main gene regulating the negative effect of NopP on nodulation, and its interaction with NopT was confirmed. These findings highlight the crosstalking network of NopT and NopP in soybean nodulation and the central role of GmPBS1.
Article
Biochemistry & Molecular Biology
Yanan Li, Zongfu Chen, Peng Zhang, Feng Gao, Junfeng Wang, Li Lin, Haibin Zhang
Summary: In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was cloned and characterized from a new sea cucumber species Psychropotes verruciaudatus (PVCuZnSOD). The PVCuZnSOD showed optimum temperature at 20 degrees C and maintained high activity from 0-60 degrees C. It also exhibited strong tolerance to various ions and chemical reagents, indicating its potential applications in medicine, food, and other products.
Article
Energy & Fuels
Yixin Ma, Yanan Li, Mengjin Zhou, Xiaoying Zhang, Zhaoyou Zhu, Yinglong Wang, Peizhe Cui, Xin Li
Summary: In this study, the separation effect of the n-heptane/ethanol system was investigated using different dihydric alcohol/dicarboxylic acid as H-Bond donors in choline-based deep eutectic solvents. Experimentally obtained x-y phase diagrams showed that the separation effect improved with longer carbon chains of H-Bond donors. Molecular simulations explored the contributions of different H-Bond donors and their interactions with the separation system. The study also developed an extractive distillation separation process using deep eutectic solvents, demonstrating the feasibility of extracting ethanol from n-heptane.
Article
Engineering, Chemical
Zhiyan Rui, Rui Ding, Xiao Duan, Xiaoke Li, Yongkang Wu, Xuebin Wang, Chen Ouyang, Jia Li, Ting Li, Jianguo Liu
Summary: Proton exchange membranes (PEMs) with high chemical durability have been researched, and the addition of ceria as a scavenger can suppress chemical degradation but leads to a decline in proton conductivity. To address this trade-off, a machine learning methodology called SPARK was developed to predict the performance and durability of ceria-containing PEMs, with broader implications for other membrane systems.
JOURNAL OF MEMBRANE SCIENCE
(2023)
