期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 56, 期 9, 页码 1021-1035出版社
WILEY-BLACKWELL
DOI: 10.1002/jobm.201600065
关键词
Lipopolysaccharide; Flagella; Escherichia coli; Outer membranes; Transcriptome
类别
资金
- National Key Basic Research Program of China [2012CB725202]
- Six Talent Peaks Project of Jiangsu Province [2012-SWYY-008]
Flagella assembly was investigated in the Escherichia coli W3110 wild-type strain and waaF, waaC, and waaG mutant strains that only synthesize lipopolysaccharide with different lengths, using transmission electron microscopy and whole genome transcriptome profiling. Under the electron microscope, the flagella were observed on the cell surface of the W3110 strain but not the waaC, waaF, or waaG strains. Transcriptional profiling showed that 1382 genes in waaC, 526 genes in waaF, and 965genes in waaG were significantly regulated compared to the control W3110 strain. These genes were further analyzed by gene ontology and KEGG pathway. Although there were significant transcriptional differences among the waaC, waaF, and waaG strains, genes related to flagella assembly and bacterial chemotaxis (the linkage between the flagella and the environment) were significantly down-regulated in all three strains. The data demonstrated that flagella assembly in E. coli depends on the length of lipopolysaccharide.
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