Article
Veterinary Sciences
Sheng Dang, Humujile Sui, Shuai Zhang, Dongxing Wu, Zeliang Chen, Jingbo Zhai, Meirong Bai
Summary: This study developed a CRISPR/CAST package for rapid detection of Brucella nucleic acid on-site, showing high accuracy, simplicity, speed, high sensitivity, and strong specificity. It is an important tool for preventing and controlling Brucella infection epidemics.
BMC VETERINARY RESEARCH
(2023)
Article
Agronomy
Xiwen Sun, Rong Lei, Haipeng Zhang, Wujian Chen, Qianwen Jia, Xing Guo, Yongjiang Zhang, Pinshan Wu, Xinyi Wang
Summary: A rapid detection method combining RPA and CRISPR-Cas12a has been developed to specifically detect two fungal pathogens causing soybean stem canker. The method demonstrates excellent specificity and sensitivity, and can be easily operated within a short time.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Kunli Zhang, Zeyi Sun, Keda Shi, Dongxia Yang, Zhibiao Bian, Yan Li, Hongchao Gou, Zhiyong Jiang, Nanling Yang, Pinpin Chu, Shaolun Zhai, Zhanyong Wei, Chunling Li
Summary: In this study, a convenient detection and analysis system for Haemophilus parasuis was established using recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR) technology. The process from sample to results can be completed within 1 hour with high sensitivity and specificity. The portable and accurate detection method holds great potential for controlling Haemophilus parasuis in the pig industry, especially in resource-limited areas.
Article
Food Science & Technology
So -Young Lee, Se-Wook Oh
Summary: The study aimed to develop a portable lateral flow biosensor for rapid and accurate detection of Salmonella in food samples using LAMP-CRISPR/Cas12a technology. The developed biosensor showed high specificity, sensitivity, convenience, mobility, low cost, and affordability.
Article
Agronomy
Dan Mark Alon, Tamir Partosh, David Burstein, Gur Pines
Summary: This study presents a CRISPR-Cas12a detection assay that can differentiate between two major pest species. The method demonstrates high specificity and sensitivity, using a simple DNA extraction technique and isothermal amplification and Cas12a-based detection. This approach can easily adapt to local threats and requires minimal training of operators, making it suitable for global implementation.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Pei Wang, Bo Guo, Xue Zhang, Yue Wang, Guang Yang, Hui Shen, Song Gao, Lihui Zhang
Summary: Acute hepatopancreatic necrosis disease (AHPND) is a devastating disease that causes significant economic losses in aquaculture worldwide. We have developed an RPA-CRISPR one-pot assay for AHPND diagnosis, which integrates RPA and CRISPR/Cas12a cleavage into simultaneous reactions. This assay has high specificity and good sensitivity, making it a new choice for AHPND diagnosis with a point-of-care testing facility and setting a good example for developing RPA-CRISPR one pot molecular diagnosis assays.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Menglu Hu, Zhiqiang Qiu, Zirong Bi, Tian Tian, Yongzhong Jiang, Xiaoming Zhou
Summary: This article proposes a CRISPR diagnostic method based on optochemical control, which addresses the issues of contamination risks and insufficient sensitivity in nucleic acid amplification-based diagnostics. The method achieves comparable detection sensitivity and specificity to PCR, and avoids contamination risks through photocontrol.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Jieru Wang, Xiaojie Zhu, Dongdong Yin, Chang Cai, Hailong Liu, Yuqing Yang, Zishi Guo, Lei Yin, Xuehuai Shen, Yin Dai, Xiaocheng Pan
Summary: First identified in China in 2019, porcine circovirus (PCV4) now poses a threat to pig health and the pig industry in various Chinese provinces and South Korea. To control the spread of infection, early detection of PCV4 is crucial. Current detection methods are labor-intensive and time-consuming. Therefore, a simple and rapid testing kit using CRISPR and Cas13a technology was developed.
Article
Microbiology
Mengqian Huang, Sihua Liu, Yanan Xu, Aqian Li, Wei Wu, Mifang Liang, Guoyu Niu, Zhiyun Wang, Tao Wang
Summary: Severe fever with thrombocytopenia syndrome virus (SFTSV), a new tick-borne pathogen, can cause severe hemorrhagic fever. In this study, a rapid and highly sensitive SFTSV detection method utilizing the CRISPR/Cas12a system and recombinase polymerase amplification was developed. The method demonstrated high sensitivity and specificity, showing 100% agreement with Q-PCR detection results for clinical samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Weidong Qian, Jie Huang, Ting Wang, Xiaoxian He, Guozhang Xu, Yongdong Li
Summary: The study developed a detection technique using RT-RPA combined with CRISPR-Cas12a for clinical diagnosis of HmPV. The RT-RPA-Cas12a based fluorescence or LF assay showed high sensitivity and specificity with a detection limit of 6.97 x 10(2) copies/mL, and no cross reaction with other common viruses.
Article
Biotechnology & Applied Microbiology
Anpeng Zhang, Bin Sun, Jianming Zhang, Can Cheng, Jihua Zhou, Fuan Niu, Zhongyong Luo, Luzhen Yu, Cui Yu, Yuting Dai, Kaizhen Xie, Qiyan Hu, Yue Qiu, Liming Cao, Huangwei Chu
Summary: This study describes a novel detection assay combining RPA and CRISPR/Cas12a for the rapid and accurate detection of A. besseyi, the causal agent of rice white tip disease. The assay is highly specific and sensitive, requires no sophisticated equipment, and shows promise for on-site surveillance.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xinyu Ling, Liying Chang, Heqi Chen, Xiaoqin Gao, Jianhang Yin, Yi Zuo, Yujia Huang, Bo Zhang, Jiazhi Hu, Tao Liu
Summary: Researchers have improved the genome editing efficiency of the Cas12a system by covalently linking CRISPR RNA and Cas12a, achieving high-efficiency gene editing and providing a new approach for engineering other low-efficiency Cas family members.
Article
Chemistry, Analytical
Yujie Liu, Hui Liu, Guanliu Yu, Wenbo Sun, Muhammad Aizaz, Guiwen Yang, Lei Chen
Summary: A highly efficient single-tube detection platform based on RPA and CRISPR reaction system has been developed to rapidly and accurately detect MRSA infection. The platform demonstrates satisfactory selectivity and can achieve detection within 30 minutes. It shows outstanding performance in sensitivity, specificity, and resistance identification.
ANALYTICA CHIMICA ACTA
(2023)
Article
Veterinary Sciences
Chao Qin, Jiajia Liu, Wenqi Zhu, Muchu Zeng, Ke Xu, Jinmei Ding, Hao Zhou, Jianshen Zhu, Yuqing Ke, Lai Yan Li, Gaoyuan Sheng, Zhuoru Li, Huaixi Luo, Shengyao Jiang, Kangchun Chen, Xianting Ding, He Meng
Summary: African swine fever virus (ASFV) is a major cause of worldwide agricultural loss, and conventional detection methods are time-consuming and instrument-dependent. In this study, a one-pot visual detection system for ASFV using CRISPR/Cas12a technology combined with LAMP or RPA was developed. The system allows rapid, sensitive, and specific detection of ASFV and can be observed by the naked eye, making it suitable for point-of-care testing.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Plant Sciences
Mei Luo, Fan-Zhu Meng, Qin Tan, Wei-Xiao Yin, Chao-Xi Luo
Summary: This study developed a highly sensitive detection method based on RPA/Cas12a, which can specifically detect Xap. The method can produce results within 2 hours and can be performed at 37 degrees Celsius, making it promising for on-site Xap diagnosis and monitoring.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Education & Educational Research
Guohua Zhang, Xiaolian Tu, Nani Ding, Joseph T. F. Lau, Pengcheng Wang, Xue Yang
Summary: This three-wave longitudinal study examined the relationships between college adjustment, self-esteem, and mental health status. The findings suggest that college adjustment and self-esteem have a moderate level of stability and are significantly correlated with mental health status. Furthermore, college adjustment and self-esteem can predict future mental health outcomes. Therefore, interventions targeting self-esteem and college adjustment may have positive effects on long-term mental health.
JOURNAL OF AMERICAN COLLEGE HEALTH
(2023)
Article
Chemistry, Applied
Wangjian Cheng, Jiaqing Mu, Kun Li, Zhanxiong Xie, Pengpeng Zhang, Chongwei An, BaoYun Ye, Jingyu Wang
Summary: Resonance acoustic mixing (RAM) technology is an attractive and safe method for material mixing, which offers high efficiency and uniformity. The study on the preparation of PBX using RAM showed that the mixing process can be divided into three stages: rough cladding, converging into a smooth ball, and flat fluidization. The PBX exhibited good uniformity and rheological behavior.
JOURNAL OF ENERGETIC MATERIALS
(2023)
Correction
Biochemistry & Molecular Biology
Hong Chen, Zhixin Cui, Wenhai Lu, Ping Wang, Jia Wang, Ziyi Zhou, Nan Zhang, Zhuo Wang, Tengfei Lin, Yun Song, Lishun Liu, Xiao Huang, Ping Chen, Genfu Tang, Yong Duan, Binyan Wang, Jianping Li, Yan Zhang, Yong Huo, Hao Zhang, Xiping Xu, Xianhui Qin, Yan Yang
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Neuroimaging
Zhaochu Sun, Ruiliang Wang, Hongquan Dong, Zheng Li, Hua Lu, Youli Hu
Summary: This study demonstrates that a prophylactic intra-arterial injection of 20 mg lidocaine can effectively and safely prevent TCR during endovascular embolization. This finding is significant for the management of TCR.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Article
Automation & Control Systems
Xinquan Zhang, Jianlin Wang
Summary: This paper studies the problem of guaranteed cost control and anti-windup design for a class of switched systems with actuator saturation. The switching strategy and anti-windup compensator are designed to ensure the stability of the closed-loop system and minimize the cost function. Sufficient conditions for the existence of an anti-windup compensator of guaranteed cost are presented using the multiple Lyapunov functions method. Based on these conditions, the minimum upper bound of the cost function is determined by solving an optimization problem under the constraint of linear matrix inequality (LMI). A numerical example is provided to demonstrate the effectiveness of the proposed method.
JOURNAL OF CONTROL AND DECISION
(2023)
Article
Nutrition & Dietetics
Xinyi Wu, Le Cui, Haoquan Wang, Jinhong Xu, Zhaozhao Zhong, Xibei Jia, Jiaqi Wang, Huahua Zhang, Yanteng Shi, Yuhang Tang, Qianhui Yang, Qiongdan Liang, Yujing Zhang, Jing Li, Xiaohong Jiang
Summary: The study compared the effects of consuming sucralose and sucrose on glucose and lipid metabolism in male mice. Results showed that sucralose had a lower impact on glucose and lipid homeostasis compared to sucrose. Sucrose consumption led to increased fat accumulation, hyperlipidaemia, liver steatosis, and glucose intolerance in mice.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Pharmacology & Pharmacy
Yun Qing, Peter F. Thall, Ying Yuan
Summary: A robust Bayesian design is proposed for monitoring a time to event endpoint in a single-arm phase II trial. Simulation results show that the piecewise exponential design has better performance than the exponential inverse gamma model based design, with higher probabilities of correctly stopping the trial early and shorter and less variable trial duration. Compared to the Weibull model based design, the piecewise exponential design does a better job of maintaining small incorrect stopping probabilities when the true median survival time is desirably large.
PHARMACEUTICAL STATISTICS
(2023)
Article
Chemistry, Multidisciplinary
Jie Li, Yan Zhou, Guy Eelen, Qing-tong Zhou, Wen-bo Feng, Viktorija Labroska, Fen-fen Ma, Hui-ping Lu, Mieke Dewerchin, Peter Carmeliet, Ming-wei Wang, De-hua Yang
Summary: The growth of solid tumors relies on tumor vascularization and endothelial cells. Targeting the glycolytic activity of endothelial cells can normalize tumor vessels, reduce metastasis, and improve chemotherapy outcomes.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Chemistry, Multidisciplinary
Xu Chi, Qian Chen, Zhi-An Lan, Xirui Zhang, Xiong Chen, Xinchen Wang
Summary: Conjugated polymers (CPs) have received increasing attention in photocatalysis due to their stability and molecular tunability. In this study, a new efficient cyano (CN) engineering approach was proposed to enhance the photocatalytic performance of CPs. Three CP-based photocatalysts with different CN contents were synthesized to investigate the relationship between CN functionalization and photocatalytic activity. Experimental and theoretical studies revealed that CN functionalization strengthened the donor-acceptor (D-A) interaction, enhanced light absorption, charge separation/transfer efficiency, hydrophilicity of CPs, and facilitated the output of photoinduced electrons from CPs to Pt cocatalyst.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Endocrinology & Metabolism
Wenjing He, Anhua Lin, Chenxiu Wang
Summary: This study found that adipocyte exosomes damage microvascular endothelial cells through the LINC00968/miR-361-5p/TRAF3 signaling pathway in the context of diabetes and obesity, which may serve as a new target for treating microvascular complications caused by diabetes and obesity.
HORMONE AND METABOLIC RESEARCH
(2023)
Proceedings Paper
Engineering, Aerospace
Jiabao Xu, Yan Wu, Qingcheng Zhu, Ping Jin, Jue Wang, Guobiao Cai
Summary: The 3D flow filling process of LOX dome in a liquid oxygen/methane rocket engine was studied, and the volume, flow field pressure, and velocity distributions of liquid oxygen at different times were determined. The flow property of propellant and the injection characteristic time of liquid oxygen were obtained. The results revealed that when the LOX dome was not filled, the flow state of LOX was disorderly and it flowed around the annular wall in the middle dome. However, after the LOX filling was completed, the liquid oxygen flow became stable and the overall velocity distribution became more uniform. There were also varying injection delays in different areas, leading to the inhomogeneity of injection characteristic time and flow rate in the thrust chamber.
13TH ASIA CONFERENCE ON MECHANICAL AND AEROSPACE ENGINEERING, ACMAE 2022
(2023)
Article
Spectroscopy
Zhang Fu, Cao Wei-hua, Cui Xia-hua, Wang Xin-yue, Fu San-ling, Zhang Ya-kun
Summary: Currently, there are few non-destructive testing models for the soluble solids content (SSC) of cherry tomatoes. This study proposes a prediction model based on the spectral characteristics of cherry tomatoes and an improved BP neural network algorithm to solve the problem of rapid non-destructive detection of cherry tomatoes' internal quality.
SPECTROSCOPY AND SPECTRAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Weitian Zhang, Hongmiao Tian, Tianci Liu, Haoran Liu, Fabo Zhao, Xiangming Li, Chunhui Wang, Xiaoliang Chen, Jinyou Shao
Summary: This study proposes a smart skin system inspired by the active color-changing mechanism of chameleons. By integrating a nanoscale hole array of photonic crystals, carbon nanotube coatings, and liquid crystal elastomers, the smart skin achieves structural color tunability, sensing, and actuation. The system autonomously modulates the color according to the environmental color and successfully demonstrates color scanning from red to green to blue. This research opens up new paths for creating active tunable structural color systems and promotes the development of structural color-based devices and systems.
MATERIALS HORIZONS
(2023)
Article
Electrochemistry
Yuying Li, Jingyi Wang, Zhihong Tian, Feili Lai, Tianxi Liu, Guanjie He
Summary: In this study, VO2/rGO composites with high electrical conductivity were successfully synthesized, and an electrochemically induced phase transition from tunneled VO2/rGO to Zn-3(OH)(2)V2O7 center dot 2H(2)O/rGO with a laminated structure and an enhanced interlayer spacing was demonstrated during the first charge to 1.6 V, showing preferable Zn2+ storage capacity. This work opens a new idea of design strategy to develop high-voltage ZIBs.
BATTERIES & SUPERCAPS
(2023)
Article
Engineering, Environmental
Jiajie Wang, Xiaoxing Wang, Mohammad S. AlQahtani, Sean D. Knecht, Sven G. Bilen, Wei Chu, Chunshan Song
Summary: This study compared CO2 hydrogenation under thermal-catalytic and plasma-catalytic conditions. The results showed significant synergy between plasma and catalyst at low temperatures, leading to increased CO2 conversion. The plasma-catalytic reaction had lower activation energy and promoted CO2 activation.
CHEMICAL ENGINEERING JOURNAL
(2023)
