Review
Food Science & Technology
Fereshteh Ansari, Shohre Alian Samakkhah, Ali Bahadori, Seyedeh Maedeh Jafari, Mojtaba Ziaee, Mohammad Taghi Khodayari, Hadi Pourjafar
Summary: Saccharomyces cerevisiae var. boulardii (S. boulardii) has been found in dairy products and possesses various beneficial activities such as anticarcinogenic, antibacterial, antiviral, and antioxidant properties. It can be used as a probiotic microorganism or starter culture in fermented dairy foods, with potential health advantages and therapeutic functions, particularly in preventing and treating gastrointestinal infectious diseases.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Adam Staniszewski, Monika Kordowska-Wiater
Summary: One approach to maintaining a healthy microbiota in the human gastrointestinal tract is through the consumption of probiotics. This study examined the probiotic potential of yeast strains isolated from Polish wines. The tested strains belonged to six different species and were evaluated for their probiotic properties, safety, enzymatic activity, and antioxidant properties. The results showed that certain strains of H. uvarum, M. pulcherrima, and S. cerevisiae exhibited promising probiotic characteristics.
Article
Biotechnology & Applied Microbiology
Pedro Pais, Jorge Oliveira, Vanda Almeida, Melike Yilmaz, Pedro T. Monteiro, Miguel C. Teixeira
Summary: The study found that S. cerevisiae var. boulardii exhibited higher survival capability and acetate biosynthesis in an intestinal-like medium compared to S. cerevisiae, possibly related to its probiotic activity. Analysis of a genomic database for S. cerevisiae var. boulardii suggested a potential link between variations in transcription factor binding sites and transcriptomic patterns.
Article
Biotechnology & Applied Microbiology
Ran Li, Xing Wan, Timo M. Takala, Per E. J. Saris
Summary: By cloning the gene encoding the antilisterial peptide leucocin C from a lactic acid bacterium into Saccharomyces boulardii, a strain was created that secreted a peptide capable of inhibiting Listeria monocytogenes. This modified S. boulardii demonstrated efficient killing of L. monocytogenes without the need for antibiotic selection pressure, suggesting its potential as a carrier for therapeutics delivery.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Review
Food Science & Technology
Adam Staniszewski, Monika Kordowska-Wiater
Summary: The paper discusses the concept of probiotic yeasts and their application in functional foods, emphasizing their positive impact on health, and pointing out future research directions.
Article
Biochemical Research Methods
Suryang Kwak, Bejan Mahmud, Gautam Dantas
Summary: This study presents a expandable and tunable transactivation system, validated in probiotic yeast Saccharomyces boulardii, allowing for precise control of functionality through the introduction of new target sequences. The system enhances orthogonality of transactivation through complementarity between promoter and scRNA, as well as binding specificity between scRNA and transcriptional activator. Additionally, control of transactivation is achieved via inducible promoter for activator expression, showing potential for precise therapeutic functions in engineered probiotic yeast.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiaojiao Xie, Mingfei Yao, Yanmeng Lu, Mengjia Yu, Shengyi Han, David J. McClements, Hang Xiao, Lanjuan Li
Summary: The study demonstrated that encapsulating Li05 within gastro-responsive microgels can enhance its efficacy in preventing and treating CDI by improving viability, release, and gut microbial diversity.
Article
Biochemistry & Molecular Biology
Jolanta Mierzejewska, Patrycja Kowalska, Klaudia Marlicka, Sara Dworakowska, Ewa Sitkiewicz, Maciej Trzaskowski, Agata Gluchowska, Grazyna Mosieniak, Malgorzata Milner-Krawczyk
Summary: This study investigated the ability of extracellular vesicles (EVs) produced by the probiotic yeast Saccharomyces boulardii to transfer and internalize bioactive molecules in human intestinal cells. The results showed that these EVs successfully delivered the anticancer drug doxorubicin to the cells without significant negative effects. Proteomic analysis also identified 541 proteins, providing important information for exploring yeast EVs as a drug delivery system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Babak Pakbin, Shaghayegh Pishkhan Dibazar, Samaneh Allahyari, Maryam Javadi, Zahra Amani, Alireza Farasat, Sina Darzi
Summary: In this study, the effects of Saccharomyces boulardii supernatant (SBS) on breast cancer cells were investigated. It was found that SBS can induce apoptosis and suppress survivin gene expression, suggesting its potential as an anticancer drug for breast carcinoma.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Biotechnology & Applied Microbiology
Jang Eun Lee, Eunjung Lee
Summary: This study aimed to discover yeast strains with intestinal anti-inflammatory activities by exhibiting probiotic properties in a DSS-induced colitis mice model. The S. cerevisiae 28-7 (SC28-7) strain was selected as a potential probiotic yeast for treating and preventing intestinal inflammatory diseases. Results showed that SC28-7 administration attenuated colon damage and inflammation, indicating its potential therapeutic value.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Mohd Akmal Azhar, Mimi Sakinah Abdul Munaim
Summary: This study used a simplex-centroid mixture design to evaluate the effect of biopolymers as excipients on probiotic tablet gastrointestinal tolerance. It identified an ideal formulation that protected probiotics and compared the stability of the tablet at different storage temperatures.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2021)
Article
Endocrinology & Metabolism
Michal Seweryn Karbownik, Joanna Kreczynska, Anna Wiktorowska-Owczarek, Paulina Kwarta, Magdalena Cybula, Nebojsa Stilinovic, Tadeusz Pietras, Edward Kowalczyk
Summary: This study aimed to investigate the effects of supplementation with Saccharomyces boulardii on salivary serotonin levels under psychological stress. The results showed that Saccharomyces boulardii supplementation decreased salivary serotonin concentration compared to placebo. Salivary serotonin was positively correlated with sympathetic-adrenal markers.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Microbiology
Alexandra Imre, Renato Kovacs, Zoltan Toth, Laszlo Majoros, Zsigmond Benko, Walter P. Pfliegler, Istvan Pocsi
Summary: This study investigates the potential role of the HMX1 gene in probiotic yeast bloodstream-derived infections by generating marker-free HMX1 deletion mutants using CRISPR/Cas9 technology. The lack of HMX1 in S. 'boulardii' increases kidney burden in mice and causes decreased growth in iron-deprived media. These findings suggest that a single-gene loss-of-function mutation can have surprising effects during an opportunistic systemic infection.
Article
Food Science & Technology
Jimmy G. Hernandez-Gomez, Argelia Lopez-Bonilla, Gabriela Trejo-Tapia, Sandra V. Avila-Reyes, Antonio R. Jimenez-Aparicio, Humberto Hernandez-Sanchez
Summary: The study evaluated the BSH activity in six probiotic strains, including five lactic acid bacteria and one probiotic yeast. Results showed that Lactobacillus plantarum DGIA1 and Saccharomyces boulardii exhibited excellent deconjugation activities against bile acids, with the yeast showing BSH activity for the first time. This finding highlights the potential of these probiotic strains in regulating cholesterol levels.
Article
Biotechnology & Applied Microbiology
JunJie Fu, Jun Liu, XuePing Wen, Guirong Zhang, Ji Cai, Zongwei Qiao, Zheming An, Jia Zheng, Li Li
Summary: This study compared the probiotic properties and metabolic differences between Saccharomyces boulardii and Saccharomyces cerevisiae. Saccharomyces boulardii showed high tolerance, antioxidant properties, and broad antibacterial properties, while also producing various beneficial metabolites. The findings have significant implications for the medical and food industries and highlight the effectiveness of metabolomics in screening microbial functional metabolites and identifying functional differences between similar microorganisms.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Chemistry, Medicinal
Sang-Woo Lee, Kim Tai Tran, Ruben Vazquez-Uribe, Charlotte Held Gotfredsen, Mads Hartvig Clausen, Blanca Lopez Mendez, Guillermo Montoya, Anders Bach, Morten Otto Alexander Sommer
Summary: CRISPR/Cas9 activity can be controlled by a small-molecule ligand identified in this study, which inhibits Cas9:gRNA complex formation by directly binding to apo-Cas9. These molecules may serve as Cas9 modulators in various applications.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Dermatology
Jonathan I. I. Silverberg, Lars E. E. French, Richard B. B. Warren, Bruce Strober, Kim Kjoller, Morten O. A. Sommer, Philippe Andres, Jakob Felding, Anne Weiss, Deniz Tutkunkardas, Tine Skak-Nielsen, Emma Guttman
Summary: This study examined the PDE4 enzymatic activity and anti-inflammatory effects of orismilast in vitro, ex vivo, and in vivo. The results showed that orismilast selectively and potently inhibited PDE4 and demonstrated broad-spectrum anti-inflammatory activity. These findings support the clinical development of oral orismilast as a novel treatment option for chronic inflammatory skin diseases.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Dermatology
Richard B. B. Warren, Bruce Strober, Jonathan I. I. Silverberg, Emma Guttman, Philippe Andres, Jakob Felding, Deniz Tutkunkardas, Kim Kjoller, Morten O. A. Sommer, Lars E. E. French
Summary: This study aimed to examine the efficacy and safety of orismilast for psoriasis treatment. The phase 2a trial found that orismilast significantly improved the condition of patients with psoriasis. The phase 1 trial tested new formulations to minimize gastrointestinal adverse reactions.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Rebecca M. Lennen, Hyun Gyu Lim, Kristian Jensen, Elsayed T. Mohammed, Patrick V. Phaneuf, Myung Hyun Noh, Sailesh Malla, Rosa A. Borner, Ksenia Chekina, Emre Ozdemir, Ida Bonde, Anna Koza, Jerome Maury, Lasse E. Pedersen, Lars Y. Schoning, Nikolaus Sonnenschein, Bernhard O. Palsson, Alex T. Nielsen, Morten O. A. Sommer, Markus J. Herrgard, Adam M. Feist
Summary: This study used an automated platform to evolve Escherichia coli to grow optimally in the presence of 11 industrial chemicals and uncovered the tolerance mechanisms. The results showed that cells are tolerized through frequent mutation of membrane transporters, cell wall-associated proteins, transcription and translation machineries, and stress signaling proteins. Osmotic stress plays a significant role in tolerance, and pre-tolerized starting isolates can greatly enhance the subsequent production of chemicals.
METABOLIC ENGINEERING
(2023)
Article
Immunology
Anne Weiss, Rodinde Hendrickx, Eva Stensgaard, Mads Jellingso, Morten O. A. Sommer
Summary: Immunocompromised patients, including dialysis and kidney transplant patients, have faced an increased risk of mortality due to COVID-19 despite available countermeasures. Additional protection measures are necessary for this vulnerable population.
Article
Biotechnology & Applied Microbiology
Karl Alex Hedin, Vibeke Kruse, Ruben Vazquez-Uribe, Morten Otto Alexander Sommer
Summary: The human gastrointestinal tract plays a crucial role in human health and microorganisms engineered for therapeutic purposes have emerged as a novel approach to managing diseases. To prevent the spread of these microorganisms outside the treated individual, safe and robust biocontainment strategies are necessary.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Akanksha Rajput, Siddharth M. Chauhan, Omkar S. Mohite, Jason C. Hyun, Omid Ardalani, Leonie J. Jahn, Morten O. A. Sommer, Bernhard O. Palsson
Summary: Lactobacillaceae is an important microbial family in the food industry. Through a comprehensive pangenome analysis, we found the characteristics of core, accessory, and rare genomes, as well as a richness of biosynthetic gene clusters.
Article
Multidisciplinary Sciences
Sarunas Tumas, Trine Sundebo Meldgaard, Troels Holger Vaaben, Sara Suarez Hernandez, Annemette Tengstedt Rasmussen, Ruben Vazquez-Uribe, Sine Reker Hadrup, Morten O. A. Sommer
Summary: In this study, we optimized the delivery of biologically active IL-2 using engineered E. coli Nissle 1917. The engineered strain showed high biological activity of microbially produced IL-2 (mi-IL2) in vitro and demonstrated strong immune cell activation in a 3D tumor spheroid model. Furthermore, the engineered strain selectively colonized tumors in a murine CT26 tumor model and resulted in a modest reduction in tumor growth rate and increased levels of IL-2 in the tumor. This study provides a workflow for researchers interested in utilizing E. coli Nissle for microbial therapy against cancer.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Chrysoula Dimopoulou, Mareike Bongers, Mikael Pedersen, Martin Bahl, Morten O. A. Sommer, Martin F. Laursen, Tine R. Licht
Summary: We successfully transferred the ability to produce the health-beneficial microbial metabolite indole lactic acid (ILA) into Escherichia coli, and demonstrated its expression in vitro and in vivo. The expanding knowledge of gut microbiota's metabolic activities and their impact on health has led to growing interest in engineered probiotics. Tryptophan metabolites, especially ILA, are potential therapeutic agents with multiple beneficial effects. Our study engineered an E. coli strain that produces ILA and showed its robust production in a mouse model, offering efficient options for therapeutic interventions against gastrointestinal inflammation.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Endocrinology & Metabolism
Valeria Iannone, Ambrin Farizah Babu, Johnson Lok, Carlos Gomez-Gallego, Giuseppe D'Auria, Ruben Vazquez-Uribe, Troels Holger Vaaben, Mareike Bongers, Santtu Mikkonen, Maija Vaittinen, Ida Tikkanen, Mikko Kettunen, Anton Klavus, Ratika Sehgal, Dorota Kaminska, Jussi Pihlajamaki, Kati Hanhineva, Hani El-Nezami, Morten Otto Alexander Sommer, Marjukka Kolehmainen
Summary: This study found that Escherichia coli Nissle 1917 expressing aldafermin, combined with dietary changes, can alleviate symptoms of MASLD, including reducing body weight, liver steatosis, plasma aspartate aminotransferase, and plasma cholesterol levels. RNA sequencing and metabolomics analysis revealed the downregulation of amino acid metabolism and related receptor signaling pathways, which may be implicated in the reduction of hepatic steatosis and insulin resistance.
MOLECULAR METABOLISM
(2023)