Article
Plant Sciences
Charlotte Permann, Klaus Herburger, Martin Niedermeier, Martin Felhofer, Notburga Gierlinger, Andreas Holzinger
Summary: Investigation on the conjugation morphology of Mougeotia spp. using various microscopy techniques revealed differences in cell wall structure between species. The zygospore walls are mainly composed of polysaccharides and lipids, with the presence of proteins, aromatic components, and other organic compounds.
Review
Cell Biology
Kinuka Ohtaka, Hiroyuki Sekimoto
Summary: Plant terrestrialization was a critical event in Earth's history, and charophytes, especially Zygnematophyceae, have been studied extensively for their phylogenetic relationship with land plants. Zygnematophyceae exhibit isogamous conjugation during sexual reproduction, a characteristic feature of this algal lineage. This study highlights three representative model organisms of Zygnematophyceae, Mougeotia, Penium, and Closterium, which serve as models for studying chloroplast photorelocation movement, cell wall structure, and sexual reproduction, respectively.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Junko Kawai, Manaki Kanazawa, Rie Suzuki, Nanako Kikuchi, Yasuhiko Hayakawa, Hiroyuki Sekimoto
Summary: The study established an efficient transformation technique for zygnematophycean algae, which not only increased transformation efficiency but also successfully performed CRISPR/Cas9-based gene knockout. This method requires minimal labor, time, and space, and can also be applied to transform other charophycean algae with available genome information.
Article
Ecology
Katerina Trumhova, Vanda Klimesova, Martina Pichrtova
Summary: This study investigated the composition and changes of Zygnema sp. assemblages in hydro-terrestrial ecosystems. The results showed that sites with stable water regimes had a more stable representation of genotypes. The study also highlighted the importance of resistant pre-akinetes in surviving winter conditions and the need to address the hidden diversity of the genus Zygnema.
Article
Plant Sciences
Tim P. Rieseberg, Armin Dadras, Luisa I. N. Bergschmidt, Maaike J. Bierenbroodspot, Janine M. R. Fuerst-Jansen, Iker Irisarri, Sophie de Vries, Tatyana Darienko, Jan de Vries
Summary: Water scarcity is a significant stressor on land, and land plants have developed avoidance and tolerance mechanisms to cope with this challenge. The Zygnematophyceae, which are closely related to land plants, exhibit similar gene expression patterns and photobiological adjustments despite their ecological differences. This study identifies differences in drought resilience between Zygnema circumcarinatum and Mesotaenium endlicherianum, shedding light on their adaptive strategies.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Environmental Sciences
Bowen Huang, Jianjun Cui, Xinyi Chen, Yongjian Huang, Cong Xu, Enyi Xie
Summary: With the rapid development of the seaweed industry in China, controlling algal blooms, especially fouling diatoms, during macroalgae industrialisation is a significant issue. This study found that Gracilaria bailiniae could effectively suppress Nitzschia closterium growth, and organic acids like citrate and hydroxyethanesulfonic acid (HA) showed allelopathic potential against N. closterium. The allelopathic mechanism of HA was found to disrupt the photosynthetic system, providing a new and eco-friendly strategy to control diatom blooms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Plant Sciences
Charlotte Permann, Burkhard Becker, Andreas Holzinger
Summary: Zygnematophyceae green algae have developed various mechanisms to adapt to temperature and light stresses on land, such as self-shading, phenolic compounds, and multilayer cell walls.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Josephine G. LoRicco, Li Kozel, Kaylee Bagdan, Ruby Epstein, David S. Domozych
Summary: In this study, the effects of endosidin 5 (ES5) on the Golgi apparatus and secretion of extracellular matrix (ECM) components in Penium margaritaceum were investigated. The results showed that ES5 had a significant impact on ECM secretion and affected the Golgi apparatus in a different way compared to other endomembrane inhibitors.
Article
Plant Sciences
Natercia Barbosa, Jean-Michel Jaquet, Oscar Urquidi, Takuji B. M. Adachi, Montserrat Filella
Summary: We studied the formation and morphology of barite crystals in Spirogyra, a freshwater alga, by combining in vitro and in vivo methods. The number and size of barite crystals were found to be related to the barium concentration in the media, and the crystals showed a crystallographic face (011) not normally observed, suggesting the influence of organic molecules on their growth kinetics. Internal and external structures of Spirogyra cells were preserved using a critical point drying method, revealing the presence of crystals near the cytoplasmic membrane, chloroplasts, and fibrillary network. In vivo optical microscopy and Raman spectroscopy in living cells showed that barite microcrystals are optically visible and follow cytoplasmic streaming. Our findings suggest that barite formation in Spirogyra occurs in the cytoplasm where both barium and sulphate are available.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Fisheries
Tingting Han, Pingping Song, Rongjun Shi, Zhanhui Qi, Jing Li, Honghui Huang
Summary: This study investigated the interactions between Sargassum hemiphyllum and Cylindrotheca closterium under different nutrient conditions. The results showed that the inhibitory effects of the two algae on each other are species-specific and dependent on nutrient availability. Inhibition was most evident under nutrient-limited conditions and was alleviated by sufficient nutrient supply. Co-cultivation resulted in decreased growth of Sargassum hemiphyllum and stimulated growth of Cylindrotheca closterium, indicating an allelopathic effect from Sargassum hemiphyllum. The competitive and inhibitory effects of Cylindrotheca closterium on Sargassum hemiphyllum were relatively lower within a certain range of nutrient concentrations.
Article
Biotechnology & Applied Microbiology
Chung Hyeon Lee, Young Kyun Lim, Ji Nam Yoon, Seung Ho Baek
Summary: This study investigated the impacts of nutrient influx and marine plastic debris on marine micro-ecosystems. The findings suggest that these inputs can alter the community structure of microalgae, increase the periphyton biomass, and potentially reduce primary productivity in the marine ecosystem.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ji Nam Yoon, Young Kyun Lim, Seung Ho Baek
Summary: A study investigated the combined effects of plastic debris and nutrients on microalgal communities in coastal waters using large scale mesocosms. The experiment showed that the introduction of nutrients resulted in a shift in dominant diatom species. Under low nutrient conditions, the periphyton community was dominated by Cylindrotheca closterium. These findings highlight the ecological importance of nutrient and land-based plastic debris inputs in coastal waters.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Environmental Sciences
Tea Misic Radic, Petra Vukosav, Bruno Komazec, Cecile Formosa-Dague, Darija Domazet Jurasin, Petra Peharec Stefanic, Andrea Cackovic, Krunoslav Juraic, Nadica Ivosevic DeNardis
Summary: This study found that polystyrene nanoparticles had negative impacts on the growth and antioxidant responses of marine diatoms, leading to decreased cell wall rigidity, excessive production of reactive oxygen species, and increased oxidative damage to lipids. The detoxifying effect of EPS and the cell abundance per NP concentration may influence the inhibitory effects of NPs on growth.
Article
Agricultural Engineering
Yuxin Wang, Jia Wang, Ziqiang Gu, Shufang Yang, Yongjin He, Haijin Mou, Han Sun
Summary: An adaptive laboratory evolution (ALE) strategy was used to transform autotrophic Nitzschia closterium into a mixotrophic strain for increased production of essential amino acid (EAA), eicosapentaenoic acid (EPA), and fucoxanthin. The ALE strategy successfully modified carbon metabolism and improved biomass concentration by 65.07% compared to the wild type. By enhancing NADPH oxidase activity, protein degradation, and carbon flux to TCA cycle, the strategy increased the production of EPA, EAA, and fucoxanthin by 41.0%, 18.8%, and 20.4% respectively. This ALE strategy shows promise for high-value microalgal production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Andreas Weber-Theen, Saeed Albaseer, Thomas Friedl, Maike Lorenz, Antje Gutowski, Laszlo Doeren
Summary: Pollution of water bodies by metals remains a threat to ecosystems, and a study examined the effects of six metals on the benthic species Closterium ehrenbergii. The study found that these benthic algae may be more sensitive to metal toxicity compared to standard planktonic species. The toxicity of the metals ranked in descending order from highest to lowest were Cu, Ag, Cd, Ni, Cr, and Zn. This study suggests that C. ehrenbergii can be a valuable addition to ecotoxicological risk assessment.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Cell Biology
Alyssa Kearly, Andrew D. L. Nelson, Aleksandra Skirycz, Monika Chodasiewicz
Summary: Stress Granules (SGs) and Processing-bodies (P-bodies) are important biomolecular condensates that play crucial roles in maintaining mRNA balance and regulating stress responses. They are composed of proteins and RNAs involved in translation, protein folding, and energy metabolism.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
P. Lemonnier, T. Lawson
Summary: Stomatal conductance plays a crucial role in determining CO2 uptake and water loss in plants, affecting overall water status and productivity. However, the signals coordinating mesophyll demands for CO2, the role of chloroplasts in stomatal function, and other GC metabolic processes in stomatal function remain poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Matteo Gionfriddo, Timothy Rhodes, Spencer M. Whitney
Summary: Rubisco is a key enzyme that facilitates the entry of CO2 into the biosphere, but its catalytic properties are slow and error-prone. More effective Rubisco variants have been discovered in certain algae, offering the potential to significantly improve crop productivity. However, incompatibilities in protein folding have hindered the transplantation of these variants into plants. Directed evolution is now being explored to enhance Rubisco catalysis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Vittoria Clapero, Stephanie Arrivault, Mark Stitt
Summary: The Calvin-Benson cycle has undergone massive selection and co-evolution with carbon-concentrating mechanisms due to changing environmental factors. Metabolite profiling reveals species-specific variations in the operation of the cycle, indicating the influence of different modes of photosynthesis. Connectivity analysis identifies constraints and driving factors for cross-species diversity in the cycle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Sukhbir Kaur, David D. Roberts
Summary: Thrombospondin-1 modulates cell behavior by interacting with components of the extracellular matrix and cell surface receptors. Its release and expression are influenced by injuries and various diseases, while its sustained presence in the extracellular space is regulated by receptor-mediated clearance. Thrombospondin-1 plays important roles in immune responses.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Richard P. Tucker, Josephine C. Adams
Summary: Thrombospondins (TSPs) play diverse roles in animals and have been found to belong to a superfamily that includes different subgroups such as mega-TSPs, sushi-TSPs, and poriferan-TSPs. Invertebrates encode a greater diversity of TSP superfamily members than vertebrates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
James Petrik, Sylvia Lauks, Bianca Garlisi, Jack Lawler
Summary: Many cancers start with a small nest of transformed cells that can remain dormant. Thrombospondin-1 (TSP-1) initially promotes dormancy by suppressing angiogenesis, but over time, factors promoting angiogenesis become dominant and recruit various cells to form a complex tumor microenvironment. TSPs play a role in the proliferation, migration, and invasion of cells in the tumor microenvironment, as well as influencing the immune characteristics and phenotype of tumor-associated macrophages.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hana Fakim, Christine Vande Velde
Summary: There has been increasing attention to the role of phase-separated biomolecular condensates, specifically stress granules, in neurodegenerative diseases like ALS. ALS-associated mutations in genes involved in stress granule assembly have been found, and stress granule proteins have been detected in pathological inclusions in ALS patient neurons. However, protein components of stress granules are also present in other physiological biomolecular condensates, which have not been adequately discussed in relation to ALS. This review explores the functions of TDP-43 and FUS in physiological condensates occurring in the nucleus and neurites beyond stress granules, and discusses the impact of ALS-linked mutations on their ability to phase separate and perform their functions in stress-independent biomolecular condensates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Alexander Lin, Yogambha Ramaswamy, Ashish Misra
Summary: Smooth muscle cells, endothelial cells, and macrophages in blood vessels display remarkable heterogeneity, and their developmental origins may influence their plasticity. Unbiased single cell whole transcriptome analysis techniques are revolutionizing the understanding of cellular diversity and plasticity, providing insights for therapeutic research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Elton P. Hudson
Summary: The Calvin Benson cycle plays a crucial role in the ecological and biotechnological aspects of bacteria. Recent studies have shed light on the regulation of this cycle in bacteria, with post-transcriptional and post-translational regulation being important in phototrophic bacteria, and transcriptional regulation being prominent in chemolithoautotrophic bacteria. Understanding the regulation of the Calvin Benson cycle has implications for enhancing CO2 fixation and improving the synthesis of desired products. Non-canonical cycles may offer potential benefits for industrial applications.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Xin-Guang Zhu, Haim Treves, Honglong Zhao
Summary: This paper discusses the major regulatory mechanisms over the Calvin Benson Cycle (CBC) that maintain homeostasis of metabolite levels. These mechanisms include redox regulation of enzymes, metabolite regulations (especially allosteric regulations), and balanced activities of enzymes. These regulatory mechanisms are crucial for maintaining high flux and photosynthetic efficiency in CBC.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hunter C. Herriage, Yi-Ting Huang, Brian R. Calvi
Summary: Apoptosis prevents the inappropriate acquisition of extra copies of the genome, known as polyploidy, but the polyploid state can suppress apoptosis. The mechanisms linking apoptosis and polyploid cell cycles are still largely unknown, and studying the regulation of apoptosis in development and cancer could lead to more effective therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Daniel Campbell, Steven Zuryn
Summary: Mitochondrial dynamics play a crucial role in regulating cellular and organismal homeostasis, impacting various aspects of an organism's healthspan. By studying the nematode Caenorhabditis elegans, a comprehensive understanding of the impact of mitochondrial dynamics on homeostasis over a lifetime can be obtained.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Diba Borgmann, Henning Fenselau
Summary: Maintaining blood glucose at an appropriate physiological level requires coordination of multiple organs and tissues, with the vagus nerve playing a key role in central control. Recent studies have revealed the cellular identity, neuroanatomical organization, and functional contributions of vagal neurons in the regulation of systemic glucose metabolism. These findings provide new insights into the precise roles of vagal neurons in coordinating glucose levels and offer potential avenues for treating glucose metabolism disorders.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Tatiana C. Coverdell, Stephen B. G. Abbott, John N. Campbell
Summary: In this article, we review how genetic technology and single-cell genomics are revealing the organizational principles of the efferent vagus in unprecedented detail.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)