Review
Nutrition & Dietetics
Xinlu Guo, Junjie Luo, Jingyi Qi, Xiya Zhao, Peng An, Yongting Luo, Guisheng Wang
Summary: The increasing elderly proportion of the population poses burdens on society, the economy, and the medical field. Polysaccharides have gained attention for their potential use in anti-aging therapies due to their natural and efficient biological properties, including antioxidant, anti-inflammatory, and immune regulatory activities.
Article
Biochemistry & Molecular Biology
Jing Sun, Xinyu Zhong, Dandan Sun, Liren Xu, Lingling Shi, Jinling Sui, Yujun Liu
Summary: This study investigated the anti-aging effects of ginsenoside residues polysaccharides (GRP) in Caenorhabditis elegans using physiological, microbiomic, and transcriptomic approaches. The results showed that GRP prolonged the lifespan of C. elegans, reduced oxidative damage caused by aging, and altered the composition of gut flora. Transcriptomic analyses also revealed potential effects of GRP on fatty acid degradation and amino acid metabolism. These findings suggest that GRP has positive effects in prolonging lifespan and alleviating aging, and may be developed as an effective dietary supplement for functional foods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Chao Xi, Mingyue Zhang, Bingtao Li, Xiaowei Meng, Shangcheng Xu, Hong Du, Xinyi Wang, Jingping Xu, Hong Ke, Yanru Cui, Fei Qu
Summary: The anti-inflammatory effects of Pueraria lobata and Pueraria thomsonii have been confirmed, and their mechanism of action involves regulating arachidonic acid metabolism and glutathione metabolism. These findings provide a scientific basis for the development of new drugs for inflammation-related diseases and lay a foundation for the utilization of herbal resources such as Pueraria lobata and Pueraria thomsonii.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shifan Zhao, Hualei Xue, Yijiong Tao, Kai Chen, Xiao Li, Mi Wang
Summary: A water-soluble acidic heteropolysaccharide, named PPL-1, was isolated from Pueraria lobata. PPL-1 had an average molecular weight of 35 kDa and consisted of glucose, arabinose, galactose, and galacturonic acid. Methylation and NMR analysis revealed the linkage patterns of various sugar residues in PPL-1. PPL-1 exhibited strong DPPH radical scavenging activity and moderate proliferation enhancement effect on RAW 264.7 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Yumin Fan, Gangliang Huang
Summary: Pueraria lobata polysaccharides (PLPs) were obtained through a hot water extraction method. The extraction process was optimized using response surface methodology, and the optimal extraction parameters were determined to be an extraction temperature of 84°C, liquid-solid ratio of 11 mL/g, extraction time of 73 min, and a polysaccharide extraction rate of 8.59%. The PLPs were then purified by removing water-soluble proteins using the Sevag method, removing pigments using H2O2, and precipitation with anhydrous ethanol. Dialysis was used to remove soluble salts and small molecules, and the refined PLPs were obtained through freeze-drying.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Ziwen Wang, Qianru Sun, Jiaxuan Fang, Changtao Wang, Dongdong Wang, Meng Li
Summary: This study compared the activity and effects of Lycium barbarum polysaccharide (LBP) extracted by hot water method and yeast fermentation method. The results showed that LBP extracted by yeast fermentation had better antioxidant and anti-aging effects, making it more suitable for applications in the food and cosmetic industries.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Na Li, Quancen Li, Xiaoyu He, Xiaoxiang Gao, Linxiu Wu, Meifang Xiao, Wenwen Cai, Bin Liu, Feng Zeng
Summary: In this study, the antioxidant activity of Laminaria japonica polysaccharide (LJP) was investigated in vitro. Additionally, the effects of LJP on thermal tolerance, oxidative stress tolerance, and aging in Caenorhabditis elegans (C. elegans) were studied in vivo. The results showed that LJP exhibited free radical scavenging activity, improved thermal tolerance, and reduced oxidative stress levels in C. elegans. Furthermore, LJP modulated the expression of certain genes and metabolic pathways, contributing to its ability to delay aging.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Food Science & Technology
Zhuang Zhang, Yushun Cui, Hui Ouyang, Weifeng Zhu, Yulin Feng, Min Yao, Shilin Yang
Summary: This study aimed to determine the protective effects of Radix Pueraria lobata polysaccharide (PPL) against ulcerative colitis (UC). The results showed that PPL inhibited inflammation in both DSS-induced UC mice and Caco-2 cells. Mechanistically, PPL inhibited inflammation by decreasing the activation of p-PI3K.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemistry & Molecular Biology
Qian Wang, Jingsong Wu, Jing Huang, Lijun Yang, Jin Tao, Jintao Nie, Jiayuan Zhao, Ya-nan Wang
Summary: Cremastra appendiculata polysaccharide (CAP) has potential anti-aging and stress resistance effects. CAP primarily comprises mannose and glucose and exhibits antioxidant activity in vitro. In vivo, CAP prolongs the lifespan of Caenorhabditis elegans in a concentration-dependent manner and enhances antioxidant enzyme activity and stress resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Ying-Hong Pei, Ning-Ning Yan, Hui-Fang Zhang, Shu-Ting Zhang, Zi-Zhong Tang, Yan Huang, Tao Chen, Shi-Ling Feng, Chun-Bang Ding, Shu Yuan, Ming Yuan
Summary: The polysaccharide extracted from Alsophila spinulosa leaves (ALP) showed excellent antioxidant and anti-aging activities, making it a potential functional food ingredient.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Ying-Jie Sun, Shi-Jie Cao, Feng-Ni Liang, Jin-Yan Li, Xiang-Yu Zhang, Wei Li, Li-Qin Ding, Feng Qiu
Summary: Five undescribed puerols and puerosides, along with three known analogues, were isolated from the roots of Pueraria lobata. Their structures were determined through comprehensive analysis of spectroscopic data and chemical methods. The absolute configurations of racemic compounds puerol D and puerol C were determined using experimental and calculated ECD spectra. The inhibitory activities of six isolates on NO generation and expression of inflammatory factors were evaluated, with (S)-puerol C, (R)-puerol C, isokuzubutenolide A, and kuzubutenolide A showing significant inhibitory effects.
Article
Biochemistry & Molecular Biology
Gaofeng Cai, Caihong Wu, Ningning Mao, Zuchen Song, Lin Yu, Tianyu Zhu, Song Peng, Yang Yang, Zhenguang Liu, Deyun Wang
Summary: This study investigated the structure of acidic Pueraria lobata polysaccharide (a-PLP) and its effects on intestinal function in cyclophosphamide (CY)-treated mice. The results showed that a-PLP relieved weight loss and intestinal villous atrophy, increased the secretion levels of immune substances, and improved the number of T cells and goblet cells. In addition, a-PLP decreased harmful levels and increased beneficial activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Kun Wang, Jingchen Sun, Jing Zhao, Yongjiao Gao, Di Yao, Daqing Sun, Mengdie Tai, Yuxi Pan, Yanjie Wang, Baoxin Lu, Feng Zuo
Summary: In this study, a capsular polysaccharide CPS-2 was isolated from Lactobacillus fermentum GBJ and characterized. CPS-2 exhibited immunomodulatory effects and anti-aging properties, making it a potential ingredient in the food or pharmaceutical industry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sai-Ya Zhang, Zi-Chen Qin, Yi-Yang Sun, Yu-Si Chen, Wen-Bo Chen, Hong-Gang Wang, Di An, Dan Sun, Yan-Qiang Liu
Summary: The study investigates the anti-heat stress and antioxidant effects of genistein in C. elegans. It is found that genistein significantly extends the lifespan of C. elegans, reduces the accumulation of lipofuscin and reactive oxygen species, and regulates the expression of aging-related genes. These effects are mediated through insulin-like growth factor signaling, heat shock protein, mitogen-activated protein kinase, dietary restriction, and mitochondrial pathways.
Article
Plant Sciences
Tisong Liang, Jiexin Zhou, Pu Jing, Zhengjun He, Shunshan Jiao, Wenji Zhao, Qi Tong, Guofu Jia
Summary: The aim of this study was to explore the relationship between phytochemical profiles and activities of Rhodiola crenulata (Rc) extracts. The study found that the 70% ethanol extracts exhibited better effects on senescence regulation and antioxidant activities. A total of 14 phytochemicals were identified in the extracts, and they might exhibit additive or synergistic effects. These findings provide a theoretical basis for the daily use of Rc.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)