Article
Biochemistry & Molecular Biology
Agata Piasecka, Andrea Baier
Summary: This study investigated the impact of three trophic modes on the growth parameters and biochemical composition of Chlorella species. The results showed that mixotrophic cultivation using an agro-industrial by-product stimulated the growth of all species, yielding high amounts of protein-rich biomass. The study also provided insights into the proteome of Chlorella cells cultured under molasses supplementation conditions.
Article
Green & Sustainable Science & Technology
Giuliano Dragone
Summary: This review explores the cultivation of different Chlorella species under mixotrophic conditions and the potential use of wastewater as a nutrient source. Common organic compounds and cheap substrates used for mixotrophic microalgal growth are presented. Developing methods to minimize contamination risk and screening novel mixotrophic Chlorella species with the ability to utilize wastewater and accumulate high-value target molecules is recommended for future commercial production.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Mahammed Ilyas Khazi, Liangtao Shi, Fakhra Liaqat, Yuxin Yang, Xin Li, Duanpeng Yang, Jian Li
Summary: The novel strategy of sequential indoor continuous mixotrophic cultivation for green cells production followed by outdoor phototrophic induction for astaxanthin accumulation showed promising results in improving biomass concentration and astaxanthin content in Haematococcus lacustris.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Greta Canelli, Sabrina Tevere, Luc Jaquenod, Fabiola Dionisi, Zhen Rohfritsch, Christoph J. Bolten, Lukas Neutsch, Alexander Mathys
Summary: This study developed a phased bioprocessing strategy and applied physical treatments to enhance the content and bioaccessibility of lipids and antioxidants in Chlorella vulgaris. The overall contents of fatty acids, carotenoids, phenolics, and alpha-tocopherol increased significantly, and the bioaccessibility of these biomolecules was improved after pulsed electric field and high-pressure homogenization treatments.
BIORESOURCE TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Tania Castillo, Diego Ramos, Tomas Garcia-Beltran, Magdalena Brito-Bazan, Enrique Galindo
Summary: Microalgae are important sources of biotechnological products, and mixotrophic growth as an alternative method can improve growth rates, biomass, and high-value compound concentrations of certain species.
Research focuses on recent studies related to mixotrophic growth of microalgae, emphasizing species that grow mixotrophically.
Furthermore, bioengineering and metabolic engineering strategies have been developed to enhance mixotrophic growth and metabolite production.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Agricultural Engineering
Chengfeng Xue, I. -Son Ng
Summary: By optimizing the process with in situ CO2 adaptation and citrate feeding strategies, biorefineries have successfully achieved efficient production of GABA and sequestered the CO2 generated in sodium hydroxide. Supplying GABA to the culture further improved biomass and lipid content.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Youping Xie, Zhen Zhang, Ruijuan Ma, Xinyu Liu, Miao Miao, Shih-Hsin Ho, Jianfeng Chen, Yoong Kit Leong, Jo-Shu Chang
Summary: This study focuses on the heterotrophic cultivation of Chlorella sorokiniana FZU60 to improve the production efficiency of lutein. Through flask cultivation and fermenter experiments, it was found that the feeding strategy 3F achieved the highest lutein production and productivity, surpassing reported values.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Shuang Wang, Jiangyi Zhou, Yifei Zhang, Sirong He, Sivakumar Esakkimuthu, Kai Zhu, Sunel Kumar, Gaojin Lv, Xun Hu
Summary: This study demonstrates the importance of using biochar and Chlorella protothecoides together for adsorbing tetracycline in environmental purification. The addition of biochar increases the biomass of Chlorella protothecoides and it proves that Chlorella protothecoides can completely absorb low concentrations of tetracycline.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Qian Feng, Yabo Sun, An Li, Xiangang Lin, Tao Lu, Dan Ding, Menghan Shi, Yingqiang Sun, Yupeng Yuan
Summary: In the short term, low concentrations of g-C3N4 promote the growth of microalgae, but long-term use is harmful. The levels of protein and lipid fractions vary at different concentrations, and changes in antioxidant enzyme activity play a significant role in microalgae growth.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
A. Sanchez-Zurano, S. Villaro-Cos, M. Ciardi, F. G. Acien-Fernandez, J. M. Fernandez-Sevilla, T. Lafarga
Summary: This study evaluates the potential use of Chlorella vulgaris for recovering nutrients from cheese whey and its impact on biomass production. The results show that Chlorella vulgaris can efficiently recover organic matter and nutrients from cheese whey, providing a sustainable alternative for the dairy industry.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Gonzalo M. Figueroa-Torres, Jon K. Pittman, Constantinos Theodoropoulos
Summary: This study establishes microalgal cultivation strategies for maximized starch and lipid formation by developing a multi-parametric kinetic model suitable for predicting mixotrophic microalgal growth dynamics co-limited by nitrogen and phosphorus. The model's high predictive capacity was experimentally validated and the identified optimal cultivation strategies significantly increased starch (+270%) and lipid (+74%) production. The findings highlight the benefits of using modeling frameworks as optimization tools for the development and commercialization of microalgae-to-fuel technologies.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Gabriela F. Ferreira, Luisa F. Rios Pinto, Rubens Maciel Filho, Leonardo V. Fregolente
Summary: This study aimed to cultivate Chlorella vulgaris and Desmodesmus sp. using sugarcane biorefinery residues bagasse and vinasse, exploring the effects of varying growth conditions. The results showed that under specific conditions, high cell density and specific metabolite accumulation can be achieved.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Hao-Cheng Yu, Chyi-How Lay, Peer Mohamed Abdul, Jane-Yii Wu
Summary: This study optimized the cultivation conditions for a local microalgae species, Chlorella sp. G3H3-1-2, with high pH-resistant, high growth rate, and high lipid content. It was found that a pH range of 6.0 to 8.0 with glucose as the carbon source resulted in the highest specific growth rate of 1.22 day(-1) for Chlorella sp. G3H3-1-2. However, the highest total FAMEs content of 59% in the biomass of 1.75 g/L was obtained when using the combination of glucose and (NH4)2SO4 at a high pH value of 10.
Article
Biotechnology & Applied Microbiology
Eleanor E. Wood, Michael E. Ross, Sebastien Jubeau, Valeria Montalescot, Karen MacKechnie, Ryan E. Marchington, Matthew P. Davey, Sharon McNeill, Callum Hamilton, Michele S. Stanley
Summary: Upscaling algal cultures from laboratory to pilot-scale is crucial for larger industrial applications, especially when screening novel algal species for biochemical and nutraceutical markets. This study focuses on Chromochloris zofingiensis, a potential competitor to Haematococcus sp. for natural astaxanthin production, and demonstrates its scalability potential using a phototrophic-mixotrophic approach.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Engineering, Environmental
Magdalena Zielinska, Paulina Rusanowska, Marcin Zielinski, Magda Dudek, Joanna Kazimierowicz, Piera Quattrocelli, Marcin Debowski
Summary: This study aimed to use membrane filtration of digestate to produce permeate for the cultivation of Chlorella vulgaris. Microfiltration resulted in the highest biomass yield due to optimum pH and nutrient concentration. Ultrafiltration reduced microalgae growth due to increased ammonia concentration. The initial ammonium concentration was found to significantly affect microalgae growth.
Article
Limnology
Niu Jianfeng, Feng Jianhua, Xie Xiujun, Gao Shan, Wang Guangce
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Songcui Wu, Xiujun Xie, Li Huan, Zhenbing Zheng, Peipei Zhao, Jixian Kuang, Xueping Liu, Guangce Wang
JOURNAL OF APPLIED PHYCOLOGY
(2016)
Article
Plant Sciences
Xiujun Xie, Aiyou Huang, Wenhui Gu, Zhengrong Zang, Guanghua Pan, Shan Gao, Linwen He, Baoyu Zhang, Jianfeng Niu, Apeng Lin, Guangce Wang
Article
Plant Sciences
X. J. Xie, X. L. Wang, L. D. Lin, L. W. He, W. H. Gu, S. Gao, X. F. Yan, G. H. Pan, M. J. Wu, G. C. Wang
Article
Marine & Freshwater Biology
Ruixue Yu, Apeng Lin, Xiujun Xie, Hui Wang, Fu Zhang, Guangzhou Liu, Guangce Wang, Cunguo Lin
PHYCOLOGICAL RESEARCH
(2016)
Article
Plant Sciences
Peipei Zhao, Wenhui Gu, Aiyou Huang, Songcui Wu, Changheng Liu, Li Huan, Shan Gao, Xiujun Xie, Guangce Wang
JOURNAL OF PHYCOLOGY
(2018)
Article
Environmental Sciences
Li Huan, Hai-xia Su, Chen-xue Duan, Shan Gao, Xiu-jun Xie, Guang-ce Wang
WATER AND ENVIRONMENT JOURNAL
(2018)
Article
Plant Sciences
L. Huan, S. Gao, X. J. Xie, W. R. Tao, G. H. Pan, B. Y. Zhang, J. F. Niu, Ap. Lin, L. W. He, G. C. Wang
Article
Plant Sciences
Xiujun Xie, Guangce Wang, Guanghua Pan, Jianzhang Sun, Jian Li
Article
Plant Sciences
Shan Gao, Wenhui Gu, Qian Xiong, Feng Ge, Xiujun Xie, Jian Li, Weizhou Chen, Guanghua Pan, Guangce Wang
PHYSIOLOGIA PLANTARUM
(2015)
Article
Plant Sciences
Shan Gao, Zhenbing Zheng, Wenhui Gu, Xiujun Xie, Li Huan, Guanghua Pan, Guangce Wang
PHYSIOLOGIA PLANTARUM
(2014)
Article
Plant Sciences
Li Huan, Xiujun Xie, Zhenbing Zheng, Feifei Sun, Songcui Wu, Moyang Li, Shan Gao, Wenhui Gu, Guangce Wang
PLANT AND CELL PHYSIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Peipei Zhao, Zhengrong Zang, Xiujun Xie, Aiyou Huang, Guangce Wang
PROCESS BIOCHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Peipei Zhao, Wenhui Gu, Songcui Wu, Aiyou Huang, Linwen He, Xiujun Xie, Shan Gao, Baoyu Zhang, Jianfeng Niu, A. Peng Lin, Guangce Wang
SCIENTIFIC REPORTS
(2014)