期刊
PARASITOLOGY RESEARCH
卷 121, 期 5, 页码 1447-1454出版社
SPRINGER
DOI: 10.1007/s00436-022-07474-y
关键词
Acanthamoeba keratitis; Contact lenses; Genotype T4; Endosymbiont; Candidatus Paracaedibacter acanthamoebae
类别
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundo de Incentivo a Pesquisa e Eventos (FIPE/HCPA)
This study reported the clinical and molecular diagnosis outcomes of Acanthamoeba keratitis (AK) cases in the southern region of Brazil during the summer of 2019. The presence of a bacterial endosymbiont in Acanthamoeba isolates requires further research to understand its potential role in the pathogenicity of Acanthamoeba infections.
Acanthamoeba keratitis (AK) is an infection that is mostly observed in contact lens wearers. It is often misdiagnosed causing delays in the administration of the correct treatment. The aim of this study was to report the outcome of clinical and molecular diagnosis of AK cases during the summer of 2019 in the southern region of Brazil. Three suspected cases of AK were discovered after an ophthalmic examination at a public hospital in the city of Porto Alegre. These cases were then confirmed through laboratory diagnosis (cell culture and molecular analysis by PCR and sequencing). In each of the three clinical sample cell cultures of corneal scraping and molecular analysis confirmed the presence of Acanthamoeba spp., all belonging to the morphological group II and to the genotype T4, which is the most common genotype associated with AK. In addition, Acanthamoeba spp. isolated from one of the clinical samples was found to harbor the Candidatus Paracaedibacter acanthamoeba, a bacterial endosymbiont. The presence of Ca. Paracaedibacter acanthamoeba in clinical isolates requires further research to reveal its possible role in the pathogenicity of Acanthamoeba infections.
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