Article
Plant Sciences
Chao Zheng, Xuming Wang, Yue Xu, Shaomin Wang, Xin Jiang, Xiuli Liu, Weijun Cui, Yueyan Wu, Chengqi Yan, Hongjia Liu, Yin Lu, Jianping Chen, Jie Zhou
Summary: Drought stress induces the expression of OsPrx114, a peroxidase gene, which enhances drought tolerance in rice by reducing reactive oxygen species (ROS) accumulation and increasing enzyme activities. Transcription factor OsWRKY50 binds to the promoter of OsPrx114 and activates its transcription, thereby regulating drought resistance in rice.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Mengqi Wang, Zhiping Qi, Wenchong Pei, Yunpeng Cheng, Ke Mao, Fengwang Ma
Summary: Salinity is a significant challenge for plants, negatively affecting their yield and quality. This study identified the MdAGO1 gene in apple, which was significantly induced under salt stress. Through transgenic approaches, it was demonstrated that MdAGO1 plays a positive regulatory role in apple's salt tolerance.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Yan-Hua Cao, Wei Ren, Hui-Juan Gao, Xin-Pei Lu, Qi Zhao, Hong Zhang, Christopher Rensing, Jin-Lin Zhang
Summary: This study discovered that HaASR2 gene from desert shrub H. ammodendron plays a critical role in plant adaptation to drought and salt stress by enhancing water use efficiency and photosynthetic capacity, maintaining the homeostasis of reactive oxygen species (ROS), and down-regulating ABA biosynthesis genes, improving Arabidopsis thaliana's tolerance to drought and salt stress.
Article
Plant Sciences
Jie Yang, Xin Guo, Weihan Li, Peihong Chen, Yunpeng Cheng, Fengwang Ma, Ke Mao
Summary: High salinity is a major environmental constraint for global agricultural productivity, and the CCX proteins play a crucial role in plant salt tolerance by inhibiting sodium accumulation and promoting ROS scavenging activities.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Yumei Liu, Qinghua Du, Longqiang Bai, Mintao Sun, Yansu Li, Chaoxing He, Jun Wang, Xianchang Yu, Yan Yan
Summary: Our results show that CsGPA1 functions as a positive regulator in cucumber plants' response to drought stress. CsGPA1 interference leads to decreased drought tolerance, with wilted leaves, lower growth rate, reduced root-shoot ratio, and decreased root activity. Physiological studies reveal higher water loss, elevated H2O2 levels, increased MDA content, and lower levels of free proline, soluble sugar, and protein in CsGPA1-interference plants under drought stress compared to wild type plants. Additionally, qRT-PCR analysis shows altered expression of AQP genes, with some up-regulated and others down-regulated in CsGPA1-interference plants, suggesting a role for CsGPA1 in modulating ROS accumulation, permeable potentials, and water loss in response to drought stress.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Plant Sciences
Yi-Ran Ren, Yu-Ying Yang, Qiang Zhao, Tian-En Zhang, Chu-Kun Wang, Yu-Jin Hao, Chun-Xiang You
Summary: MdCIB1 plays a positive role in enhancing drought resistance in plants. It also participates in blue light response and flowering time regulation. Additionally, MdCIB1 overexpression improves physiological indexes under drought stress and enhances ABA sensitivity and ROS scavenging capabilities.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Huan Zhang, Zhen Wang, Xu Li, Xiaoru Gao, Zhuoru Dai, Yufei Cui, Yuhai Zhi, Qingchang Liu, Hong Zhai, Shaopei Gao, Ning Zhao, Shaozhen He
Summary: The study revealed that the transcription factor IbBBX24 regulates the expression of peroxidase gene IbPRX17, enhancing sweet potato tolerance to salt and drought stresses. Overexpression lines of IbBBX24 and IbPRX17 showed higher peroxidase activity and lower H2O2 accumulation under abiotic stress conditions. Additionally, the interaction between IbBBX24 and APETALA2 protein IbTOE3 was found to improve the response to abiotic stress in sweet potato and tobacco plants.
Review
Plant Sciences
Hadi Pirasteh-Anosheh, Maryam Samadi, Seyed Abdolreza Kazemeini, Munir Ozturk, Agnieszka Ludwiczak, Agnieszka Piernik
Summary: Reactive oxygen species (ROS) are important for seed germination and various physiological processes in plants. While their functions have been extensively studied in common plants, little is known about their role in germination of halophyte seeds. This review aims to unravel the mechanisms of ROS homeostasis in halophytes and dry seeds, as well as ROS flux during germination of halophyte seeds.
Article
Plant Sciences
Kuo Yang, Chong-Yang Li, Jian-Ping An, Da-Ru Wang, Xun Wang, Chu-Kun Wang, Chun-Xiang You
Summary: The study found that the apple MdZAT10 gene encoding a zinc finger protein plays a negative regulator role in plant drought resistance. This protein affects plant growth and biochemical characteristics under drought stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Bingxin Wang, Liqun Li, Mingliu Liu, De Peng, Aosong Wei, Beiyuan Hou, Yanhong Lei, Xuejun Li
Summary: This study discovered a gene, TaFDL2-1A, in wheat that can improve drought resistance by regulating ABA biosynthesis, ABA responses, and ROS scavenging.
Article
Forestry
Yuanyuan Jing, Changhai Liu, Bingbing Liu, Tingting Pei, Minghui Zhan, Chunrong Li, Duanni Wang, Pengmin Li, Fengwang Ma
Summary: This study demonstrates that MdMRLK2 gene regulates drought tolerance in apple trees by modulating levels of energetic matters, free amino acids, and abscisic acid.
Article
Plant Sciences
Chhaya Yadav, Rajeev Nayan Bahuguna, Om Parkash Dhankher, Sneh L. Singla-Pareek, Ashwani Pareek
Summary: This study investigates the response of different rice genotypes to combinations of drought, heat, and salinity stress. The findings suggest that sensitive genotypes exhibit higher damage and reduced photosynthetic efficiency under stress, while tolerant genotypes perform better under specific stress combinations. Gene expression analysis reveals the importance of different genes in response to stress combinations. Overall, this study highlights the unique response of rice to stress combinations at the physiological and molecular levels.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Plant Sciences
Dandan Li, Jingli Yang, Solme Pak, Minzhen Zeng, Jiali Sun, Sen Yu, Yuting He, Chenghao Li
Summary: A novel regulatory pathway for drought tolerance in Populus ussuriensis roots was discovered, where PuC3H35 mediated proanthocyanidin and lignin biosynthesis by collaboratively regulating various gene modules. This pathway enhances plant adaptation to drought stress by promoting antioxidant activity and mechanical support.
Article
Biochemistry & Molecular Biology
Xiaotong Wei, Xuhong Fan, Honglin Zhang, Peng Jiao, Zhenzhong Jiang, Xuan Lu, Siyan Liu, Shuyan Guan, Yiyong Ma
Summary: In this study, the stress-related gene Zm00001d019704 (ZmSRG7) was identified and its biological function in maize was determined. The results showed that ZmSRG7 gene can improve maize tolerance to drought or salt stress by regulating hydrogen peroxide homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Jingjing Chang, Yanliang Guo, Jingyi Yan, Zixing Zhang, Li Yuan, Chunhua Wei, Yong Zhang, Jianxiang Ma, Jianqiang Yang, Xian Zhang, Hao Li
Summary: The study reveals that ClCOMT1 gene in watermelon plays a crucial role in regulating melatonin biosynthesis and enhancing plant tolerance to abiotic stresses.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Xiushan Mao, Nan Hou, Zhenzhong Liu, Jieqiang He
Summary: This study evaluated the m(6)A modification in apple trees in response to drought stress and analyzed its association with transcript expression. The results showed that m(6)A modification was highly enriched in the 3' untranslated region and coding sequence in apple trees, and a significant number of differentially methylated genes were identified under drought conditions. The differential m(6)A-modified genes were found to be associated with RNA processing, epigenetic regulation, and stress tolerance.
Article
Plant Sciences
Giulia Pasqualetto, Luisa Palmieri, Stefan Martens, Vincent G. M. Bus, David Chagne, Claudia Wiedow, Mickael A. Malnoy, Susan E. Gardiner
Summary: Apple and pear are economically important fruit crops known for their unique qualities. This research utilized comparative genomic approaches to identify genetic differences and metabolite patterns, validating the effectiveness of three marker types in detecting hybridity between apple and pear. The findings will enhance breeding of new tree fruit crops, benefiting producers and consumers through marker assisted selection of desired traits between pear and apple.
HORTICULTURE RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Luca Nerva, Lorenza Dalla Costa, Angelo Ciacciulli, Silvia Sabbadini, Vera Pavese, Luca Dondini, Elisa Vendramin, Emilia Caboni, Irene Perrone, Andrea Moglia, Sara Zenoni, Vania Michelotti, Sabrina Micali, Stefano La Malfa, Alessandra Gentile, Stefano Tartarini, Bruno Mezzetti, Roberto Botta, Ignazio Verde, Riccardo Velasco, Mickael Arnaud Malnoy, Concetta Licciardello
Summary: Climate change is affecting food chain production, leading to reduced quality and yield. The international scientific community is working on enhancing resilience in agriculture. Italy, a major European producer of fruit trees, has taken initiatives to maintain the specificity of the 'Made in Italy' label. However, conventional breeding approaches face challenges in terms of financial commitment, land resources availability, and long generation times.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Pengda Cheng, Qianyu Yue, Yutian Zhang, Shuang Zhao, Abid Khan, Xinyue Yang, Jieqiang He, Shicong Wang, Wenyun Shen, Qian Qian, Wanshan Du, Fengwang Ma, Dehui Zhang, Qingmei Guan
Summary: GABA improves fruit quality and enhances drought tolerance in apple, increasing sugar-to-acid ratio, flesh firmness, pericarp malleability, and GABA content, while reducing fruit acidity. Pretreatment of apple rootstock M.9-T337 with GABA improves its tolerance to long-term and short-term drought stress.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiangping Mao, Chundong Niu, Ke Li, Li Fan, Zhimin Liu, Shaohuan Li, Doudou Ma, Muhammad Mobeen Tahir, Libo Xing, Caiping Zhao, Juanjuan Ma, Na An, Mingyu Han, Xiaolin Ren, Dong Zhang
Summary: This study confirmed the relationship between cytokinin and adventitious root formation in apple rootstocks. It was found that the expression of MdTCP17 and MdWOX11 was involved in the regulation of cytokinin-mediated inhibition of adventitious root primordium formation. The results provide valuable insights into the molecular mechanisms underlying this process.
Article
Plant Sciences
Simon Miranda, Stefano Piazza, Floriana Nuzzo, Mingai Li, Jorge Lagreze, Axel Mithoefer, Alessandro Cestaro, Danuse Tarkowska, Richard Espley, Andrew Dare, Mickael Malnoy, Stefan Martens
Summary: The effects of targeting the key gene MdPGT1 involved in phloridzin biosynthesis in apple using conventional transgenesis and CRISPR/Cas9-mediated genome editing were simultaneously assessed. Knockdown lines showed impaired growth and leaf morphology, while genome-edited lines exhibited normal growth despite reduced phloridzin levels. Transcriptomic and metabolic analyses revealed regulated genes involved in phenylpropanoid and flavonoid pathways. Phytohormone profiling demonstrated differential modulation, with increased salicylic and jasmonic acid levels in dwarf lines and no correlation between auxin and ABA with growth phenotype.
Article
Plant Sciences
Xiaoxia Shen, Yi Song, Yikun Ping, Jieqiang He, Yinpeng Xie, Fengwang Ma, Xuewei Li, Qingmei Guan
Summary: Apple trees often face various non-biotic and biotic stresses, and traditional approaches have limited progress in developing cold-hardy and disease-resistant cultivars. Biotechnology has been shown to be a feasible approach to improve stress tolerance in woody perennial plants. This study reveals that MdHYL1 plays a positive role in cold tolerance and pathogen resistance in apple.
Editorial Material
Plant Sciences
Jun Wu, Lailiang Cheng, Richard Espley, Fengwang Ma, Mickael Malnoy
Article
Plant Sciences
Umberto Salvagnin, Katharina Unkel, Thorben Sprink, Paul Bundock, Robert Sevenier, Milica Bogdanovic, Sladana Todorovic, Katarina Cankar, Johanna Christina Hakkert, Elio Schijlen, Ronald Nieuwenhuis, Maria Hingsamer, Veronika Kulmer, Michael Kernitzkyi, Dirk Bosch, Stefan Martens, Mickael Malnoy
Summary: In this study, different delivery methods of CRISPR/Cas9 reagents were compared for their efficiency in genome editing of chicory, as well as the frequency of off-target mutations and their environmental and economic impacts. The study found that using RNPs as the delivery method resulted in high editing efficiency, no off-target mutations, and avoided the integration of unwanted plasmid DNA and the need for gene segregation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ambra S. Parmagnani, Chidananda Nagamangala Kanchiswamy, Ivan A. Paponov, Simone Bossi, Mickael Malnoy, Massimo E. Maffei
Summary: The bacterial phytopathogen Erwinia amylovora produces volatile organic compounds (mVOCs) that enhance Arabidopsis thaliana growth. These mVOCs trigger early signaling events and modulate the expression of genes involved in plant growth and defense responses.
Article
Horticulture
Qianyu Yue, Jieqiang He, Xinyue Yang, Pengda Cheng, Abid Khan, Wenyun Shen, Yi Song, Shicong Wang, Fengwang Ma, Qingmei Guan
Summary: Apples can be categorized into different ripening cultivars based on the length of the fruit developmental phases. In this study, the transcriptomics of early and late-ripening apple cultivars were studied to identify differential genes related to fruit ripening. The results showed that both cultivars require similar processes for fruit development, but the timing of gene expression and enriched pathways were different. This study contributes to a better understanding of the molecular basis of apple ripening and has implications for apple breeding using genomics.
Article
Plant Sciences
Manuela Campa, Simon Miranda, Concetta Licciardello, Justin Graham Lashbrooke, Lorenza Dalla Costa, Qingmei Guan, Armin Spok, Mickael Malnoy
Summary: Climate change and invasive pathogens pose challenges for the fruit industry, driving the development of new breeding techniques. This review explores accelerated breeding, cisgenesis, and CRISPR/Cas genome editing as promising options for improving fruit tree traits. The optimization and diversification of CRISPR/Cas tools are discussed, along with protoplast regeneration and delivery techniques. The regulatory landscape and social acceptability of these techniques are also considered.
Article
Plant Sciences
Jidi Xu, Jieqiang He, Bichun Hu, Nan Hou, Junxing Guo, Caixia Wang, Xuewei Li, Zhongxing Li, Jingjing Zhai, Ting Zhang, Chuang Ma, Fengwang Ma, Qingmei Guan
Summary: This study reveals the involvement of mRNA N-6-methyladenosine (m6A) modification in the apple grafting process and shows its contribution to various biological processes such as RNA processing, epigenetic regulation, stress response, and development. The downregulation of m(6)A methyltransferase gene MdMTA leads to global m(6)A hypomethylation and the upregulation of DNA demethylase gene MdROS1, suggesting a possible association between m(6)A and 5mC methylation in apples.
Article
Plant Sciences
Qianyu Yue, Xinyue Yang, Pengda Cheng, Jieqiang He, Wenyun Shen, Yixuan Li, Fengwang Ma, Chundong Niu, Qingmei Guan
Summary: MdKING1, a subunit of SnRKs, promotes fruit ripening through ethylene pathway.
Article
Plant Sciences
Ofere Francis Emeriewen, Klaus Richter, Annette Wensing, Mickael Malnoy, Andreas Peil
Summary: A strong fire blight resistance QTL (Mfu10) was previously detected on linkage group 10 (LG10) of Malus fusca accession MAL0045, and another QTL (Mfu16) was detected on LG16 using a mutant strain Ea1038. Progenies carrying both Mfu10 and Mfu16 showed significantly higher resistance than those carrying only Mfu10. However, Mfu16 was less stable in a larger subset of individuals.
JOURNAL OF PLANT PATHOLOGY
(2023)
