期刊
CANCER EPIDEMIOLOGY
卷 39, 期 1, 页码 37-41出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.canep.2014.11.003
关键词
Larynx; Hypopharynx; Squamous cell carcinoma; Human papillomavirus; Immunohistochemistry; PCR; In situ hybridization
资金
- Plan Nacional de I+D+I Instituto de Salud Carlos III [PI11-929, EMER07-048]
- Plan Nacional de I+D+I ISCIII [CP13/00013, PI13/00259]
- Red Tematica de Investigacion Cooperativa en Cancer (RTICC), Spain [RD12/0036/0015]
- FEDER Funding Program from the European Union
Background: Recent studies support a role for human papillomavirus (HPV) in oropharyngeal squamous cell carcinomas (SCCs); however, the significance of HPV in non-oropharyngeal head and neck cancers is uncertain. The aim of this study was to determine the prevalence of HPV in a large cohort of laryngeal and hypopharyngeal SCCs in northern Spain. Materials and methods: Clinical records and paraffin-embedded tumor specimens of 124 consecutive patients surgically treated for laryngeal (62 cases) and hypopharyngeal (62 cases) SCCs between 2002 and 2007 were retrieved. All cases were histologically evaluated, and presence of HPV was assessed by p16-immunohistochemistry followed by GP5+/6+-PCR-based DNA detection. Samples positive in both assays were subjected to HPV genotyping and HPV E6 transcript analysis. Results: Seventeen cases (14%) were positive for p16 immunostaining, of which 2 (1 larynx, 1 hypopharynx, 1.6% of total series) were found positive for HPV DNA by subsequent GP5+6+-PCR. Both SCCs contained HPV type 16 and showed HPV16 E6 mRNA expression. Conclusions: HPV is only occasionally involved in laryngeal and hypopharyngeal SCC patients in northern Spain. (C) 2014 Elsevier Ltd. All rights reserved.
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