Article
Biochemistry & Molecular Biology
Angela Maione, Alessandra La Pietra, Antonietta Siciliano, Aldo Mileo, Maria De Falco, Elisabetta de Alteriis, Marco Guida, Emilia Galdiero
Summary: Candida tropicalis is an emerging pathogen with a high mortality rate due to its virulent factors. The biofilm formation ability of C. tropicalis highlights the urgent need for novel antifungal agents. In this study, the arylamidine T-2307 showed antibiofilm capacity and increased survival in infected larvae. It did not affect the viability of PNT1A cells and inhibited the secretion of inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Carmen de Jesus-Gil, Lidia Sans-de San Nicolas, Ester Ruiz-Romeu, Marta Ferran, Laura Soria-Martinez, Irene Garcia-Jimenez, Anca Chiriac, Josep Manel Casanova-Seuma, Josep Manel Fernandez-Armenteros, Sherry Owens, Antonio Celada, Michael D. Howell, Ramon Maria Pujol, Luis Francisco Santamaria-Babi
Summary: The study found that patients with plaque psoriasis have higher levels of anti-CA IgA and IgG in their plasma, and the cellular response to Candida albicans primarily occurs in CLA(+) T cells, particularly Th17 cells. In plaque psoriasis, the levels of anti-CA IgA are directly associated with the CLA(+) Th17 response. Proteomic analysis revealed distinct profiles in psoriasis patients with high anti-CA IgA levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Yinhe Mao, Norma Solis, Anupam Sharma, Max Cravener, Scott G. Filler, Aaron P. Mitchell
Summary: Genetic strategies have been essential for understanding biological processes at a mechanistic level. In this study, the authors describe a genetic tool for the fungal pathogen Candida albicans, which can be used to evaluate gene function and pathway relationships through engineered conditional gene expression. The tool utilizes the RBT5 promoter and has been validated in multiple clinical isolates, demonstrating its potential usefulness in the infection environment.
Article
Pharmacology & Pharmacy
Khaled M. Hosny, Amal M. Sindi, Sarah Ali, Waleed S. Alharbi, Maher S. Hajjaj, Haitham A. Bukhary, Moutaz Y. Badr, Rayan Y. Mushtaq, Samar S. A. Murshid, Alshaimaa M. Almehmady, Rana B. Bakhaidar, Eman Alfayez, Mallesh Kurakula
Summary: This study developed a nanostructured lipid carrier of sesame oil loaded with miconazole to overcome solubility issues and enhance its antifungal activity against oral candidiasis. The optimized formulation showed improved efficacy in inhibiting the growth of Candida albicans both in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Gabriela Rios Martini, Ekaterina Tikhonova, Elisa Rosati, Meghan Bialt Decelie, Laura Katharina Sievers, Florian Tran, Matthias Lessing, Arne Bergfeld, Sophia Hinz, Susanna Nikolaus, Julia Kuempers, Anna Matysiak, Philipp Hofmann, Carina Saggau, Stephan Schneiders, Ann-Kristin Kamps, Gunnar Jacobs, Wolfgang Lieb, Jochen Maul, Britta Siegmund, Barbara Seegers, Holger Hinrichsen, Hans-Heinrich Oberg, Daniela Wesch, Stefan Bereswill, Markus M. Heimesaat, Jan Rupp, Olaf Kniemeyer, Axel A. Brakhage, Sascha Brunke, Bernhard Hube, Konrad Aden, Andre Franke, Iliyan D. Iliev, Alexander Scheffold, Stefan Schreiber, Petra Bacher
Summary: Aberrant CD4(+) T cell reactivity against intestinal microorganisms is thought to drive mucosal inflammation in inflammatory bowel diseases. This study identifies yeasts as direct activators of altered CD4(+) T cell reactions in patients with Crohn's disease, revealing their potential role in chronic activation of inflammatory immune responses.
Review
Chemistry, Multidisciplinary
Ulla I. M. Gerling-Driessen, Miriam Hoffmann, Stephan Schmidt, Nicole L. Snyder, Laura Hartmann
Summary: Pathogens, including viruses, bacteria, fungi, and parasites, have a significant impact on our lives. Understanding their mechanisms of adhesion and invasion of host cells has led to the development of glycan-based diagnostics and therapeutics. Glycopolymers, macromolecules with carbohydrates as side chain motifs, have become increasingly important due to their tunable properties. In this review, we discuss the role of glycopolymers in pathogen adhesion and infection, biofilm formation and removal, and drug delivery, as well as their use as diagnostics for pathogen detection and monitoring.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Immunology
Mengyun Hu, Samuele Notarbartolo, Mathilde Foglierini, Sandra Jovic, Federico Mele, David Jarrossay, Antonio Lanzavecchia, Antonino Cassotta, Federica Sallusto
Summary: T follicular helper (T-FH) cells are crucial for promoting B cell responses and antibody affinity maturation in germinal centers (GC). This study investigates the antigen specificity and relationship between circulating T-FH (cT(FH)) cells and other memory CD4(+) T cells. The findings show that cT(FH) and non-cT(FH) subsets contain cells specific to different antigens, with distinct frequencies and effector functions. Flu-specific cT(FH) cells exhibit a GC T-FH-like phenotype, while the repertoire of Flu-specific cT(FH) and non-cT(FH) cells differs. Furthermore, Flu-specific cT(FH) and non-cT(FH) cells persist as stable repertoires for years, providing insights into the heterogeneity and persistence of antigen-specific human cT(FH) cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Environmental Sciences
Kana Morinaga, Toshiki Nagakubo, Nobuhiko Nomura, Masanori Toyofuku
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Ryo Nagasawa, Tsutomu Sato, Nobuhiko Nomura, Tomoyo Nakamura, Hidenobu Senpuku
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Tomohiro Hirayama, Kyosuke Takabe, Tatsunori Kiyokawa, Nobuhiko Nomura, Yutaka Yawata
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Biotechnology & Applied Microbiology
Nozomu Obana, Kouji Nakamura, Nobuhiko Nomura
NPJ BIOFILMS AND MICROBIOMES
(2020)
Article
Biotechnology & Applied Microbiology
Ryo Nagasawa, Tatsuya Yamamoto, Andrew S. Utada, Nobuhiko Nomura, Nozomu Obana
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Materials Science, Biomaterials
Kyoka Komaba, Nobuhiko Nomura, Hiromasa Goto
Summary: Living organisms use signal messengers to respond to stimuli, and 3-oxo-C12-HSL is a messenger molecule that plays a role in bacterial colonies. In this study, a conductive polymer was successfully prepared by electrochemical polymerization and composite formation using the crystal form of 3-oxo-C12-HSL as a template. The achievement of electrochemical polymerization in the crystal structure of a QS material is significant in this research.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2022)
Article
Biology
Gayan Abeysinghe, Momoka Kuchira, Gamon Kudo, Shunsuke Masuo, Akihiro Ninomiya, Kohei Takahashi, Andrew S. Utada, Daisuke Hagiwara, Nobuhiko Nomura, Naoki Takaya, Nozomu Obana, Norio Takeshita
LIFE SCIENCE ALLIANCE
(2020)
Article
Biotechnology & Applied Microbiology
Sachiko Sakamoto, Masaru K. Nobu, Daisuke Mayumi, Satoshi Tamazawa, Hiroyuki Kusada, Hideharu Yonebayashi, Hiroki Iwama, Masayuki Ikarashi, Tatsuki Wakayama, Haruo Maeda, Susumu Sakata, Tomohiro Tamura, Nobuhiko Nomura, Yoichi Kamagata, Hideyuki Tamaki
Summary: A novel anaerobic thermophilic bacterium, strain NRmbB1(T), was isolated from a deep subsurface oil field in Japan. This bacterium showed unique cellular structure, growth conditions, and metabolic pathways. Based on chemotaxonomic, phylogenetic, and genomic characterization, a new genus, family, and order were proposed for this bacterium.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Toshiki Nagakubo, Yuhei O. Tahara, Makoto Miyata, Nobuhiko Nomura, Masanori Toyofuku
Summary: The study utilized super-resolution live-cell imaging and biochemical analysis to reveal that Corynebacterium glutamicum forms different types of bacterial membrane vesicles (MVs) via different routes in response to environmental conditions. DNA-damaging stress and envelope stress induced MV formation through distinct mechanisms, which were found to be conserved in other mycolic acid-containing bacteria. This research provides insights into how the complex cell envelope structure intrinsically generates various types of MVs in single cell organisms.
Article
Microbiology
Kimihiro Abe, Masanori Toyofuku, Nobuhiko Nomura, Nozomu Obana
Summary: Bacillus subtilis releases membrane vesicles (MVs) under various stress conditions, with autolysins playing a key role in this process. The autolysis serves as a novel and prophage-independent pathway for MV production in B. subtilis. Exogenous purified MVs can effectively neutralize cell damage induced by surfactant treatment.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Divya Naradasu, Waheed Miran, Shruti Sharma, Satoshi Takenawa, Takamitsu Soma, Nobuhiko Nomura, Masanori Toyofuku, Akihiro Okamoto
Summary: The study demonstrated the biogenesis of OMVs in C. ochracea and characterized the size and protein profile of OMVs. The blebbing mechanism was suggested as a specific process for OMV generation, indicating a different biogenesis mechanism compared to other bacteria. The study provides insights into the roles of C. ochracea OMVs in oral biofilms and systemic diseases.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Jiayue Yang, Yongshou Yang, Manami Ishii, Mayuko Nagata, Wanping Aw, Nozomu Obana, Masaru Tomita, Nobuhiko Nomura, Shinji Fukuda
MICROBES AND ENVIRONMENTS
(2020)
Article
Multidisciplinary Sciences
Yukino Ogawa, Chika Miyoshi, Nozomu Obana, Kaho Yajima, Noriko Hotta-Hirashima, Aya Ikkyu, Satomi Kanno, Tomoyoshi Soga, Shinji Fukuda, Masashi Yanagisawa
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Tomohiro Inaba, Nozomu Obana, Hiroshi Habe, Nobuhiko Nomura
MICROBES AND ENVIRONMENTS
(2020)
Article
Chemistry, Multidisciplinary
Yousuke Kikuchi, Nozomu Obana, Masanori Toyofuku, Noriyuki Kodera, Takamitsu Soma, Toshio Ando, Yoshihiro Fukumori, Nobuhiko Nomura, Azuma Taoka