Article
Microbiology
Samanta Freire, Teresa Grilo, Bruna Rodrigues, Rui Oliveira, Carla Esteves, Antonio Marques, Laurent Poirel, Marta Aires-de-Sousa
Summary: This study aimed to analyze the occurrence and characteristics of ESBL- and carbapenemase-producing E. coli among bivalves. Out of 522 collected bivalve samples, E. coli growth was observed in 39% of the samples. Nine samples (4.4%) were contaminated with ESBL producers and one sample (0.5%) was contaminated with a carbapenemase-producing K. pneumoniae. The presence of ESBL- and carbapenemase-producing Enterobacteriaceae in bivalves indicates that the marine environment could be a reservoir of critical bacterial pathogens, posing a potential risk to human health.
Article
Immunology
Achiraya Siriphap, Thawatchai Kitti, Akachai Khuekankaew, Chalermchai Boonlao, Chonthida Thephinlap, Chutamas Thepmalee, Nittiya Suwannasom, Krissana Khoothiam
Summary: This study investigated the prevalence of ESBL-positive E. coli and K. pneumoniae isolates from hospitalized patients in Chiangrai Prachanukroh hospital, Thailand. The study found a high prevalence and emerging antibiotic resistance of ESBL-positive strains, indicating the need for carbapenems and aminoglycosides as the first-line drugs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Lin Huang, Min Cao, Yanyan Hu, Rong Zhang, Yufei Xiao, Gongxiang Chen
Summary: The trend of fosfomycin resistance among KPC-KP strains is increasing in China, with the two major mechanisms of resistance being plasmid-mediated fosfomycin resistance gene fosA3 and mutation of the target gene glpT.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Jose Joao Sumbana, Antonella Santona, Nader Abdelmalek, Maura Fiamma, Massimo Deligios, Alice Manjate, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: Globally, antibiotic-resistant Klebsiella spp. cause healthcare-associated infections with high mortality rates. The study investigated the phenotypic and genetic features of Klebsiella isolates from invasive infections in Mozambique. The majority of isolates displayed multidrug resistance, with ESBL-producing strains carrying various beta-lactamase genes. The study also identified susceptible hypervirulent Klebsiella pneumoniae isolates associated with severe infections. The findings highlight the importance of surveillance strategies to prevent the emergence of multidrug-resistant hypervirulent Klebsiella pneumoniae.
Article
Immunology
F. Morales-Leon, A. Opazo-Capurro, C. Caro, N. Lincopan, A. Cardenas-Arias, F. Esposito, V. Illesca, M. L. Rioseco, M. Dominguez-Yevenes, C. A. Lima, H. Bello-Toledo, Gerardo Gonzalez-Rocha
Summary: The convergence of virulence and multidrug-resistant phenotype is reported for the first time in Chile in Klebsiella pneumoniae complex isolates, indicating a clinical problem that requires special attention and continuous surveillance in South America. The detected hypervirulent isolates displayed resistance to broad-spectrum cephalosporins and carried multiple antibiotic-resistance genes, leading to a multidrug-resistant phenotype. In vivo virulence testing confirmed higher virulence of the isolates compared to the control strain.
Article
Multidisciplinary Sciences
Chonticha Romyasamit, Phoomjai Sornsenee, Siriphorn Chimplee, Sitanun Yuwalaksanakun, Dechawat Wongprot, Phanvasri Saengsuwan
Summary: The study found ESBL-producing E. coli and K. pneumoniae in raw vegetables, showing high resistance to beta-lactam antibiotics but good sensitivity to beta-lactam/carbapenem antibiotics. The genes carried by these bacteria may be associated with food chains and humans, suggesting that raw vegetables could serve as an important route for the transmission of ESBL-resistant bacteria to humans.
Article
Infectious Diseases
Carolina Sabenca, Eliana Costa, Sara Sousa, Lillian Barros, Ana Oliveira, Sonia Ramos, Gilberto Igrejas, Carmen Torres, Patricia Poeta
Summary: The emergence of Klebsiella pneumoniae strains producing ESBL and KPC has become a major public health concern. The ability of these strains to form biofilms is worrisome as it can facilitate the spread of antibiotic resistance and prolong infections in healthcare facilities.
Article
Infectious Diseases
Tyler J. Stone, Abdullah Kilic, John C. Williamson, Elizabeth L. Palavecino
Summary: The in vitro activity of omadacycline and comparator antibiotics against clinical ESBL-producing and non-ESBL-producing E. coli and K. pneumoniae urinary isolates was evaluated. Results showed that 54.9% of all ESBL-producing isolates and 74.5% of ESBL-producing E. coli isolates were susceptible to omadacycline. Overall, omadacycline and nitrofurantoin were the most active agents for treating UTI caused by ESBL-producing E. coli.
Article
Public, Environmental & Occupational Health
Anouk P. Meijs, Esther F. Gijsbers, Paul D. Hengeveld, Cindy M. Dierikx, Sabine C. de Greeff, Engeline van Duijkeren
Summary: The study found that despite the absence of specific occupation-related risk factors, the carriage of ESBL-E/K among veterinary healthcare workers was high, indicating that occupational contact with animals may be a potential source of ESBL-E/K for the general population.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Engineering, Multidisciplinary
Cemile Bagkur, Emrah Guler, Bilgen Kaymakamzade, Evren Hincal, Kaya Suer
Summary: While antibiotic resistance is a growing concern, this study focuses on the future clinical situation of ESBL-producing K. pneumoniae. By using mathematical modeling, the study evaluates the severity of the issue and identifies critical points to prevent the spread of the ESBL-producing strain. The results show that the use of antibiotics and hospital admissions play a major role in the spread of ESBL-producing K. pneumoniae, and recovery from infections is the most effective way to limit its spread.
CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES
(2022)
Article
Microbiology
M. Ortiz-Padilla, I Portillo-Calderon, B. de Gregorio-Iaria, J. Blazquez, J. Rodriguez-Bano, A. Pascual, J. M. Rodriguez-Martinez, F. Docobo-Perez
Summary: This study aimed to characterize the role of specific genes in fosfomycin resistance in Klebsiella pneumoniae and assess the potential synergistic effect of combining fosfomycin with sodium phosphonoformate. The results showed that inactivation of the fosA gene led to decreased fosfomycin resistance, while the addition of PPF had limited effects and showed decreased activity in the presence of fosfomycin-resistant mutations.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Rajnikant Sharma, Estefany Garcia, John K. Diep, Vince H. Lee, Faisal Minhaj, Brian Jermain, Evelyn J. Ellis-Grosse, Cely S. Abboud, Gauri G. Rao
Summary: This study aimed to determine the pharmacodynamic and immunomodulatory effects of fosfomycin alone and in combination with polymyxin B against KPC-2-producing Klebsiella pneumoniae clinical isolates. The results showed that combination therapy had better bactericidal efficacy and reduced the expression of cytokines induced by bacterial infection. These findings highlight the importance of the immune response in antimicrobial therapy and provide new insights for further research on immune-mediated killing mechanisms.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Immunology
Lisandra Aguilar-Bultet, Claudia Bagutti, Adrian Egli, Monica Alt, Laura Maurer Pekerman, Ruth Schindler, Reto Furger, Lucas Eichenberger, Tim Roloff, Ingrid Steffen, Philipp Huebner, Tanja Stadler, Sarah Tschudin-Sutter
Summary: This report describes a cluster of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sequence type 101, derived from 1 poultry and 2 clinical samples collected within the setting of a prospective study designed to determine the diversity and migration of ESBL-producing Enterobacterales between humans, foodstuffs, and wastewater.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
F. Gravey, S. Wilson, A. Denisenko, C. Lehoussel, Marguerite Fines-Guyon, S. Le Hello, C. Jeanne-Leroyer
Summary: Whole genome sequencing is now the standard method used for microbiological investigations, enabling the detection of undisclosed outbreaks. By utilizing this approach, we were able to investigate and ultimately halt an uncommon epidemic caused by the extended-spectrum beta-lactamase-producing Klebsiella pneumoniae ST584 strain that spanned over four months in two intensive care units.
Article
Infectious Diseases
Alice Elena Ghenea, Ovidiu Mircea Zlatian, Oana Mariana Cristea, Anca Ungureanu, Radu Razvan Mititelu, Andrei Theodor Balasoiu, Corina Maria Vasile, Alex-Ioan Salan, Daniel Iliuta, Mihaela Popescu, Anca-Loredana Udristoiu, Maria Balasoiu
Summary: CTX-M β-lactamases have become the most prevalent ESBLs in Enterobacteriaceae. This study identified the prevalence of bla(CTX-M-15), bla(SHV-1), and bla(TEM-1) encoding β-lactamases in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains in a Romanian hospital's ICU. The findings highlight the importance of reducing antibiotic use and improving resistance surveillance to guide local antibiotic therapy.
Article
Infectious Diseases
Toshiharu Sasaki, Yohei Doi
Summary: This study investigated cases of S. lugdunensis bacteremia and found that the clinical significance of single-positive cases is uncertain. Among all cases, only a small subset of low-risk patients did not require antimicrobial therapy, and they had no adverse clinical consequences.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Yu Takahashi, Hideaki Wakita, Takuma Ishihara, Hideto Okazaki, Akihiro Ito, Mitsunaga Iwata, Shigeru Sonoda, Yohei Doi
Summary: Healthcare-associated COVID-19 among vulnerable patients leads to disproportionate morbidity and mortality. Early pharmacologic intervention may reduce negative sequelae and improve survival in such settings. This study aimed to describe outcome of patients with healthcare-associated COVID-19 who received early short-course remdesivir therapy.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Hiroyuki Naruse, Yohei Doi, Mitsunaga Iwata, Kiyohito Ishikawa
Summary: During the COVID-19 pandemic, Japan's early return-to-work (RTW) program for healthcare workers (HCWs) who have been in close contact with COVID-19 cases was found to be a reasonable strategy. HCWs eligible for the program had received the third dose of a COVID-19 mRNA vaccine, tested negative for COVID-19 before each work shift, and were difficult to replace. Out of 256 HCWs identified as close contacts, 37 secondary cases were detected, and 141 HCWs (55%) participated in the early RTW program, with no reported infection clusters.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Microbiology
Chuning Wang, Zhewei Sun, Yiyi Hu, Dan Li, Qinglan Guo, Minggui Wang
Summary: The study found that sequence type (ST) 15 has become an emerging clone of carbapenem-resistant Klebsiella pneumoniae, in which type I-E* CRISPR-Cas usually exists. This suggests that the CRISPR-Cas system may not be able to block the transfer of bla(KPC) plasmids. The purpose of the study was to explore the mechanisms underlying dissemination of bla(KPC) plasmids in K. pneumoniae ST15.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Leilei Wang, Xuefei Zhang, Xun Zhou, Yingmin Bi, Minggui Wang, Qinglan Guo, Fan Yang
Summary: Eleven bla(PER-1)-positive Pseudomonas aeruginosa clinical isolates exhibited variable susceptibility to ceftazidime-avibactam (CZA). The genetic contexts of bla(PER-1) were identical except for one isolate, which showed increased resistance due to an insertion in the upstream region of bla(PER-1). The variability in bla(PER-1) promoter activity partially explains the different susceptibility to CZA in PER-producing isolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Pharmacology & Pharmacy
Jue Wang, Pei Li, Lin Xin, Xiaogang Xu, Minggui Wang
Summary: We reported the presence of vanM vancomycin resistance gene in Shanghai in 2006, and found it to be the main van gene in vancomycin-resistant enterococci (VRE). In this study, we collected 1292 Enterococcus faecium and Enterococcus faecalis strains from Huashan Hospital, Fudan University, and determined that most of them were vancomycin-sensitive. However, using a modified disk diffusion test, we found 10 E. faecium isolates that were previously considered vancomycin-sensitive to have colonies in the vancomycin inhibition zone. These isolates were later found to be vanM-positive. The disk diffusion-based method can help detect vanM-positive E. faecium with low vancomycin minimum inhibitory concentrations and prevent missing the detection of vancomycin sensitivity-variable enterococci.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2023)
Article
Immunology
Angelique E. Boutzoukas, Lauren Komarow, Liang Chen, Blake Hanson, Souha S. Kanj, Zhengyin Liu, Soraya Salcedo Mendoza, Karen Ordonez, Minggui Wang, David L. Paterson, Scott Evans, Lizhao Ge, Abhigya Giri, Carol Hill, Keri Baum, Robert A. Bonomo, Barry Kreiswirth, Robin Patel, Cesar A. Arias, Henry F. Chambers, Vance G. Fowler, David van Duin
Summary: This study described the clinical, molecular epidemiology, and outcomes of Carbapenemase-producing Escherichia coli (CP-Ec) from several countries. The study found regional variations in CP-Ec and called for strengthened monitoring and treatment measures. Patients infected with metallo-beta-lactamase (MBL) producers had lower mortality rates.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Mylene Vaillancourt, Anna Clara Milesi Galdino, Sam P. Limsuwannarot, Diana Celedonio, Elizabeth Dimitrova, Matthew Broerman, Catherine Bresee, Yohei Doi, Janet S. Lee, William C. Parks, Peter Jorth
Summary: This study investigates the virulence mechanisms of antibiotic resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients. It reveals that a mutation in the RNase E gene leads to increased production of siderophores, which damage macrophage cell membranes and cause cell death. The study also suggests that gallium can reduce the virulence of RNase E variants and may serve as a precision therapy for these infections.
Article
Immunology
Rick van der Geest, Hongye Fan, Hernan F. Penaloza, William G. Bain, Zeyu Xiong, Naina Kohli, Emily Larson, Mara L. G. Sullivan, Jonathan M. Franks, Donna B. Stolz, Ryota Ito, Kong Chen, Yohei Doi, Melanie J. Harriff, Janet S. Lee
Summary: This study found heterogeneity in the sensitivity of clinical Kp isolates to macrophage phagocytic uptake, and demonstrated that phagocytosis is a significant determinant of the pathogenicity of Kp in the lungs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Gayatri Shankar Chilambi, Yu-Hao Wang, Nathan R. Wallace, Chetachukwu Obiwuma, Kirsten M. Evans, Yanhong Li, Menna-Allah W. Shalaby, Daniel P. Flaherty, Ryan K. Shields, Yohei Doi, Daria Van Tyne
Summary: This study identified a mutant strain of Enterococcus faecalis that is hypersusceptible to aminoglycoside antibiotics due to disruption of alpha-carbonic anhydrase (alpha-CA). Disruption of alpha-CA increased proton motive force and membrane permeability, leading to increased gentamicin uptake in E. faecalis. Additionally, an FDA-approved alpha-CA inhibitor sensitized various E. faecalis strains to gentamicin.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Infectious Diseases
Luke S. P. Moore, Maria Virginia Villegas, Eric Wenzler, Timothy M. Rawson, Rita O. Oladele, Yohei Doi, Anucha Apisarnthanarak
Summary: Despite variable utilization of rapid diagnostic tests (RDTs) in antimicrobial stewardship programs (ASPs) across different income countries, a value framework has been proposed to define the benefits of RDTs in ASPs, separate from per-patient benefits. Effective implementation is key to realizing the value of RDTs within ASPs, and actionable advice for choosing an RDT has been proposed by infectious disease experts from various countries. These experts also advocate for the inclusion of RDTs in the World Health Organization Model List of essential in vitro diagnostics and in the iterative development of national action plans.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Immunology
Minggui Wang, Lizhao Ge, Liang Chen, Lauren Komarow, Blake Hanson, Jinnethe Reyes, Eric Cober, Thamer Alenazi, Zhiyong Zong, Qing Xie, Zhengyin Liu, Lanjuan Li, Yunsong Yu, Hainv Gao, Souha S. Kanj, Jairo Figueroa, Erica Herc, Ezequiel Cordova, Gregory Weston, Paul Ananth Tambyah, Julia Garcia-Diaz, Keith S. Kaye, Sorabh Dhar, Jose M. Munita, Robert A. Salata, Samuel Vilchez, Martin E. Stryjewski, Maria Virginia Villegas Botero, Alina Iovleva, Scott R. Evans, Keri Baum, Carol Hill, Barry N. Kreiswirth, Robin Patel, David L. Paterson, Cesar A. Arias, Robert A. Bonomo, Henry F. Chambers, Vance G. Fowler, Michael J. Satlin, David van Duin, Yohei Doi
Summary: In this international, prospective observational cohort of 842 hospitalized patients with carbapenem-resistant Acinetobacter baumannii, infection types and clinical outcomes differed significantly across regions. Although clonal group 2 (CG2) strains were predominant, non-CG2 strains were associated with higher mortality.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Nadine Rouphael, Patricia Winokur, Michael C. Keefer, Jessica Traenkner, Ana Drobeniuc, Yohei Doi, Sonal Munsiff, Vance G. Fowler, Scott Evans, Randolph E. Oler, Bonifride Tuyishimire, Marina Lee, Varduhi Ghazaryan, Henry F. Chambers
Summary: Concerns over resistance and safety have been identified in the current treatment regimen for complicated urinary tract infections. Fosfomycin is a drug that is routinely used for the treatment of uncomplicated cystitis. This study shows that fosfomycin could be an oral alternative as step-down therapy for the treatment of complicated urinary tract infections, with a clinical cure rate comparable to levofloxacin but a lower microbiological success rate 3 weeks from start of antibiotics.
Article
Microbiology
Rauf Salamzade, Christi L. McElheny, Abigail L. Manson, Ashlee M. Earl, Nader Shaikh, Yohei Doi
Summary: Escherichia coli is the most common cause of urinary tract infections (UTIs) in children. However, the mechanisms of virulence and antibiotic resistance, as well as the population structure of this species, are poorly understood in this age group. This study examined the genetic markers and population structure of uropathogenic E. coli (UPEC) in children with UTIs. The results revealed similarities between UPEC populations in children and adults, with certain genetic markers being more common in pediatric pyelonephritis cases. Additionally, antibiotic resistance was correlated with known genetic markers, and a fimbrial gene allele was found to confer a selective advantage for UPEC.
Article
Microbiology
Richard D. Smith, Min Zhan, Shanshan Zhang, Surbhi Leekha, Anthony Harris, Yohei Doi, Scott Evans, J. Kristie Johnson, Robert K. Ernst
Summary: This study evaluated a new rapid lipid analysis technique called FLAT MS for direct identification of pathogens from blood cultures. FLAT MS showed slight differences in accuracy compared to other FDA-approved methods for identifying Gram-positive bacteria, Gram-negative bacteria, and yeast. The time-to-results and hands-on time of FLAT MS were comparable to other methods.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
