Macromolecular crowding limits growth under pressure

Cells that grow in confined spaces eventually build up mechanical compressive stress. This growth-induced pressure decreases cell growth. Growth-induced pressure is important in a multitude of contexts, including cancer(1-3), microbial infections(4) and biofouling(5); yet, our understanding of its origin and molecular consequences remains limited. Here we combine microfluidic confinement of the yeast Saccharomyces cerevisiae(6) with rheological measurements using genetically encoded multimeric nanoparticles(7) to reveal that growth-induced pressure is accompanied with an increase in a key cellular physical property: macromolecular crowding. We develop a fully calibrated model that predicts how increased macromolecular crowding hinders protein expression and thus diminishes cell growth. This model is sufficient to explain the coupling of growth rate to pressure without the need for specific molecular sensors or signalling cascades. As molecular crowding is similar across all domains of life, this could be a deeply conserved mechanism of biomechanical feedback that allows environmental sensing originating from the fundamental physical properties of cells.

Automated summary

The study reveals that cellular growth in confined spaces leads to mechanical compressive stress, which in turn decreases cell growth. This growth-induced pressure is accompanied by an increase in a cellular physical property called macromolecular crowding. The researchers develop a model that explains how increased macromolecular crowding hinders protein expression and reduces cell growth, without the need for specific molecular sensors or signaling cascades. This mechanism could be a conserved feedback loop that allows cells to sense their environment based on their physical properties.