Article
Infectious Diseases
Dan Li, Pei Li, Xiaoyan Yu, Xuefei Zhang, Qinglan Guo, Xiaogang Xu, Minggui Wang, Minghua Wang
Summary: This study investigated the molecular characteristics of Escherichia coli causing bloodstream infections in Huashan Hospital, Shanghai, China from 2010 to 2015. The results showed that E. coli strains isolated from BSI were still highly susceptible to carbapenems and beta-lactamase inhibitor combinations, with bla(CTX-M) being the dominant genotype of ESBLs. The low expression of bla(KPC) may be the reason for the lower resistance of E. coli to carbapenems.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Medicine, General & Internal
Yanli Liu, Minli Zhu, Xiaoqin Fu, Jiaojiao Cai, Shangqin Chen, Yuanyuan Lin, Na Jiang, Si Chen, Zhenlang Lin
Summary: The study found that Escherichia coli remains the primary pathogen causing neonatal meningitis at a perinatal center in eastern China. There were significant changes in the susceptibility of E. coli to third-generation cephalosporins, with a correlation between this change and ESBL production. The percentage of ESBL-producing multidrug-resistant E. coli isolated from infants with early-onset meningitis was significantly lower than that from infants with late-onset meningitis.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Article
Infectious Diseases
Michael T. Birrell, Kylie Horne, Benjamin A. Rogers
Summary: Escherichia coli is a common cause of bacteremia, leading to a wide range of clinical conditions. This study aimed to evaluate the impact of antimicrobial stewardship (AMS) interventions on the management of E. coli bacteremia. Results showed that AMS interventions could reduce the total days of antimicrobial therapy and optimize the duration of treatment for patients with E. coli bacteremia.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Editorial Material
Microbiology
Johann D. D. Pitout
Summary: Escherichia coli is a major cause of bloodstream infections worldwide. A longitudinal study in a Madrid hospital found that certain E. coli B2 phylogroups dominated the community E. coli population and were often introduced into hospital settings. Longitudinal surveys from England and Canada also showed that certain ST131 subclades were responsible for increased resistance to fluoroquinolones and cephalosporins in E. coli during the mid- to late 2000s.
Article
Biochemistry & Molecular Biology
Sumeet Manandhar, Amy Scott-Thomas, Michael Harrington, Priyanka Sinha, Anna Pilbrow, Arthur Mark Richards, Vicky Cameron, Madhav Bhatia, Stephen T. Chambers
Summary: This study measured the concentrations of hydrogen sulfide (H2S) and substance P (SP) in the peripheral blood of patients with Escherichia coli or Klebsiella pneumoniae bacteraemia. The study found that the concentrations of H2S and SP varied with the site of infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Jonas Bredtoft Boel, Valeria Antsupova, Jenny Dahl Knudsen, Jens Otto Jarlov, Magnus Arpi, Barbara Juliane Holzknecht
Summary: The study suggests that IV mecillinam may be a suitable targeted treatment for bacteraemia with a urinary tract focus, but these results need confirmation by randomized controlled studies.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Rasha El-Mahdy, Rasha Mahmoud, Raghdaa Shrief
Summary: The study aimed to characterize different uropathogenic E. coli (UPEC) phylogroups from catheter-associated UTI (CAUTI) and community UTI (Com-UTI) in terms of antibiotic resistance, biofilm formation, and pathogenicity islands (PAIs). UPEC strains from CAUTI and Com-UTI showed similar high antibiotic resistance, with higher biofilm formation in CAUTI strains. Phylogroup A was the most common, and PAI IV536 was the prevalent marker in all phylogroups from both types of UTI.
INFECTION AND DRUG RESISTANCE
(2021)
Review
Infectious Diseases
Gisele Peirano, Yasufumi Matsumara, Diego Nobrega, Rebekah DeVinney, Johann Pitout
Summary: Escherichia coli ST1193 is an emerging global clone associated with fluoroquinolone resistance, responsible for increasing incident blood stream infections among elderly females in LTC centers in a centralized Canadian region. The rapid emergence of ST1193 with bla(CTX-Ms) in 2018 is concerning and adds to the public health burden of multidrug resistant E. coli blood stream infections in Calgary.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Rebecca A. Gladstone, Alan McNally, Anna K. Pontinen, Gerry Tonkin-Hill, John A. Lees, Kusti Skyten, Francois Cleon, Martin O. K. Christensen, Bjorg C. Haldorsen, Kristina K. Bye, Karianne W. Gammelsrud, Reidar Hjetland, Angela Kummel, Hege E. Larsen, Paul Christoffer Lindemann, Iren H. Lohr, Ashild Marvik, Einar Nilsen, Marie T. Noer, Gunnar S. Simonsen, Martin Steinbakk, Stale Tofteland, Marit Vattoy, Stephen D. Bentley, Nicholas J. Croucher, Julian Parkhill, Pal J. Johnsen, Orjan Samuelsen, Jukka Corander
Summary: This study found that CC131 clade A isolates, predominantly antimicrobial susceptible, were established early and sustained, indicating that resistance is not necessary for clonal success. However, in the low antibiotic use setting of Norway, resistance to important antimicrobial classes has rapidly been selected for in CC131 clade A isolates. This underscores the importance of genomic surveillance in understanding the complex ecology of multidrug resistance dissemination and competition.
Article
Infectious Diseases
Rasmus Richelsen, Jesper Smit, Pavithra Laxsen Anru, Henrik Carl Schonheyder, Henrik Nielsen
Summary: This study investigated temporal changes in risk factors of community-onset ESBL-producing E. coli and K. pneumoniae bacteraemia in North Denmark. High-risk factors included use of fluoroquinolones, frequent hospitalization, and exposure to antibiotics. Temporal analysis indicated a slight reduction in factors associated with direct healthcare contact.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Chemistry, Multidisciplinary
Yohei Nakamura, Kaede Okita, Daisuke Kudo, Dao Nguyen Duy Phuong, Yoshihito Iwamoto, Yoshie Yoshioka, Wataru Ariyoshi, Ryota Yamasaki
Summary: Magnesium hydroxide nanoparticles, especially the smaller NM80, have demonstrated a significant bactericidal effect against Escherichia coli, including both exponential cells and persister cells. The bactericidal activity is attributed to physical cell damage, observed through scanning electron microscopy. This makes NM80 a promising antibacterial agent.
Article
Biochemical Research Methods
Yu Kang, Lina Yuan, Xing Shi, Yanan Chu, Zilong He, Xinmiao Jia, Qiang Lin, Qin Ma, Jian Wang, Jingfa Xiao, Songnian Hu, Zhancheng Gao, Fei Chen, Jun Yu
Summary: This study investigates genome-wide recombination within a species using a dataset of Escherichia coli genomes. The research highlights extensive within-clade recombination and a sharp decline in between-clade recombination, establishing a genetic barrier between clades as phylogenetic distance increases. Recombination plays a critical role in early speciation by optimizing genes and alleles for physiological activities and shaping genetic diversity within populations.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Pediatrics
Borja Elgoibar, Iker Gangoiti, Juan Jose Garcia-Garcia, Susanna Hernandez-Bou, Borja Gomez, Lorea Martinez Indart, Santiago Mintegi
Summary: This study characterized the emergency department presentations of pediatric E coli bacteraemia and identified four different types with varying severity. Febrile young children with associated UTI showed the best outcomes, while non-well-appearing children of different ages had higher severity rates, including fatalities.
Article
Pediatrics
Borja Elgoibar, Iker Gangoiti, Juan Jose Garcia-Garcia, Susanna Hernandez-Bou, Borja Gomez, Lorea Martinez Indart, Santiago Mintegi
Summary: The study identified four different types of presentations of pediatric E coli bacteremia in the emergency department, with varying levels of severity. Feverish young children with associated UTI showed the best outcomes, while older children with underlying diseases and children with severe symptoms had higher mortality rates.
Article
Environmental Sciences
Luisa Andrade, Martin Boudou, Paul Hynds, Carlos Chique, John Weatherill, Jean O'Dwyer
Summary: This study analyzed groundwater monitoring data in the Republic of Ireland and found high contamination rates of Escherichia coli in groundwater, with associations to factors such as seasonality, geological features, and rainfall. The results are expected to inform policy change and groundwater monitoring programs in geologically heterogeneous regions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Infectious Diseases
Shazad Mushtaq, Anna Vickers, Michel Doumith, Matthew J. Ellington, Neil Woodford, David M. Livermore
Summary: Taniborbactam potentiates partner beta-Lactams against various Enterobacterales with different carbapenemases, showing potential broader spectrum compared to vaborbactam.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Katherine A. Twohig, Tommy Nyberg, Asad Zaidi, Simon Thelwall, Mary A. Sinnathamby, Shirin Aliabadi, Shaun R. Seaman, Ross J. Harris, Russell Hope, Jamie Lopez-Bernal, Eileen Gallagher, Andre Charlett, Daniela De Angelis, Anne M. Presanis, Gavin Dabrera
Summary: A study in England found that patients with COVID-19 infected with the delta variant were more likely to be admitted to hospital or attend emergency care compared to those infected with the alpha variant. This suggests that outbreaks of the delta variant in unvaccinated populations may pose a greater burden on healthcare services.
LANCET INFECTIOUS DISEASES
(2022)
Article
Immunology
Nina J. Zhu, Timothy M. Rawson, Siddharth Mookerjee, James R. Price, Frances Davies, Jonathan Otter, Paul Aylin, Russell Hope, Mark Gilchrist, Yeeshika Shersing, Alison Holmes
Summary: The COVID-19 pandemic has affected the patterns of community- and hospital-acquired bloodstream infections in COVID-19 and non-COVID-19 patients. There was a high incidence of hospital-acquired bacteremia during the COVID-19 waves, especially in SARS-CoV-2-negative elective patients.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Stephanie Evans, James Stimson, Diane Pople, Alex Bhattacharya, Russell Hope, Peter J. White, Julie Robotham
Summary: Despite evidence of nosocomial transmission of SARS-CoV-2 in hospitals, the contributions of different transmission pathways are poorly quantified. This study analyzed national hospital records and found that indirect transmission between patients, such as through healthcare workers or contaminated objects, was the most likely route of nosocomial transmission. The risk of transmission to patients from healthcare workers is low but significant when the number of infected patients is low. The risk of healthcare workers acquiring SARS-CoV-2 in the hospital is equal to that in the community.
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
(2022)
Article
Infectious Diseases
Martin J. Llewelyn, Detelina Grozeva, Philip Howard, Joanne Euden, Sarah M. Gerver, Russell Hope, Margaret Heginbothom, Neil Powell, Colin Richman, Dominick Shaw, Emma Thomas-Jones, Robert M. West, Enitan D. Carrol, Philip Pallmann, Jonathan A. T. Sandoe
Summary: Introduction of procalcitonin testing in emergency departments/acute medical units in England was associated with an initial decrease in antibiotic use per admission per week per trust, but this effect was not sustained over time. The introduction of procalcitonin testing in the ICU setting did not result in a significant change in antibiotic use. Further research is needed to determine the patient-level impact of procalcitonin testing and its potential clinical effectiveness.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Shirin Aliabadi, Elita Jauneikaite, Berit Muller-Pebody, Russell Hope, Karina-Doris Vihta, Carolyne Horner, Ceire E. Costelloe
Summary: This study provides a comprehensive epidemiological picture of E. coli bacteraemia resistance trends and risk factors in England. The findings highlight the increasing rates of antibiotic resistance over the study period and identify age as a significant risk factor. These results have important implications for guiding future prescribing policies for antimicrobial agents.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Michel Doumith, Sarah Alhassinah, Abdulrahman Alswaji, Maha Alzayer, Essa Alrashidi, Liliane Okdah, Sameera Aljohani, Hanan H. Balkhy, Majed F. Alghoribi
Summary: Carbapenem-resistant P. aeruginosa is a major clinical problem in Saudi Arabia. This study provides insights into the genetic characteristics and antibiotic resistance mechanisms of these clinical isolates, highlighting the global spread of specific sub-lineages. It emphasizes the importance of routine detection of rare beta-lactamases.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Katie L. Hopkins, Nicholas Ellaby, Matthew J. Ellington, Michel Doumith, Nazim Mustafa, Daniele Meunier, Neil Woodford
Summary: By sequencing CPE samples from the national reference laboratory in England, we found that the epidemiology of CPE in England is diverse, indicating multiple introductions and a role for horizontal transfer of carbapenemase genes.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Maha Alzayer, Majed F. Alghoribi, Bassam Alalwan, Abdualah Alreheli, Sameera Aljohani, Mohammad Bosaeed, Michel Doumith
Summary: This study investigated the in vitro activity of Cefiderocol against carbapenem non-susceptible Gram-negative bacteria collected in Saudi Arabia. The results showed that Cefiderocol exhibited excellent activity, especially against multidrug-resistant non-fermenting Gram-negative pathogens.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Evolutionary Biology
Emma V. Waters, Liam A. Tucker, Jana K. Ahmed, John Wain, Gemma C. Langridge
Summary: Bacterial genome structure rearrangements around long repeat sequences can cause changes in gene expression. Multiplexed long-read sequencing enables the identification of genome structure rearrangements, which may provide a mechanism for bacteria to quickly adapt to new environments.
Article
Infectious Diseases
Katie Harman, Sophie Grace Nash, Harriet H. Webster, Natalie Groves, Jo Hardstaff, Jessica Bridgen, Paula B. Blomquist, Russell Hope, Efejiro Ashano, Richard Myers, Sakib Rokadiya, Susan Hopkins, Colin S. Brown, Meera Chand, Gavin Dabrera, Simon Thelwall
Summary: There are concerns about the reduced efficacy of sotrovimab in reducing hospitalisation risk for the BA.2 sub-lineage of the Omicron variant. A retrospective study was conducted to compare the risk of hospitalisation between BA.2 and BA.1 cases treated with sotrovimab. The results suggest that the risk of hospital admission was similar for both sub-lineages.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2023)
Article
Microbiology
Emma McGuire, Claire Neill, Simon M. Collin, Hannah Higgins, Rebecca Guy, Mark Ganner, Juliana Coelho, Bruno Pichon, Russell Hope, Colin S. Brown
Summary: PVL toxin detection was not associated with worse outcomes in patients with community-acquired S. aureus bacteremia.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Letter
Infectious Diseases
Nurin Abdul Aziz, Sophie Grace Nash, Asad Zaidi, Tommy Nyberg, Natalie Groves, Russell Hope, Jamie Lopez Bernal, Gavin Dabrera, Simon Thelwall
JOURNAL OF INFECTION
(2023)
Article
Infectious Diseases
Maha Alzayer, Manal M. Alkhulaifi, Ahmed Alyami, Mohammed Aldosary, Abdulaziz Alageel, Ghada Garaween, Atef Shibl, Arif M. Al-Hamad, Michel Doumith
Summary: Group B Streptococcus (GBS) has emerged as an important cause of severe infections in adults. This study investigated the epidemiology of GBS in Saudi Arabia and found that serotypes III and V were the most prevalent, and most isolates harbored pilus island 1 and 2a types. The isolates exhibited high levels of resistance to erythromycin and clindamycin, which require further surveillance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Antoine Abou Fayad, Maria El Azzi, Ahmad Sleiman, Issmat I. Kassem, Reema A. Bawazeer, Liliane Okdah, Michel Doumith, Majed F. Alghoribi, Ghassan M. Matar
Summary: The study revealed the presence of mcr and other important resistance determinants in MDR E. coli isolated from poultry farms in Lebanon, with resistance genes predominantly located on IncX4 plasmids. Some strains may pose a risk of transmission to humans.
JAC-ANTIMICROBIAL RESISTANCE
(2021)