Article
Multidisciplinary Sciences
Sorawit Na Nongkhai, Phitchayakon Piemthongkham, Wichanee Bankeeree, Hunsa Punnapayak, Pongtharin Lotrakul, Sehanat Prasongsuk
Summary: This study aimed to enhance the value of sugarcane leaf by extracting xylan and producing xylooligosaccharide (XO) through enzymatic hydrolysis. The xylan was successfully extracted using alkali extraction, and the resulting arabinoxylan had high molecular weight. Enzymatic hydrolysis with endoxylanase yielded XOs with specific prebiotic activity towards certain strains of Lactobacillus spp.
Article
Biotechnology & Applied Microbiology
Tianfu Li, Wenwen Yang, Xingran Xu, Yan Zhang, Jie Chen, Xiang Zou
Summary: The study investigated the coproduction of PMA and liamocins from waste by-products from the xylitol and gluconate industries, showing that waste xylose mother liquor could be used as a cost-effective feedstock without pretreatment. High titers of PMA and liamocins were obtained in batch fermentation, with economic assessment revealing the process to be economically viable and sustainable.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemical Research Methods
Kenneth C. Nwoko, Xinjin Liang, Magali A. M. J. Perez, Eva Krupp, Geoffrey Michael Gadd, Joerg Feldmann
Summary: The study recovered and characterized selenium (Se) and tellurium (Te) nanoparticles (NPs) using asymmetric flow field flow fractionation (AF4) coupled with various detectors. Different buffer solutions and flow rates were compared for optimal separation and recovery of NPs, with Novachem solution showing higher stability for elution. The results demonstrate the effectiveness of complementary techniques in characterizing biogenic Se and Te NPs.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Materials Science, Characterization & Testing
Fabian Hernandez-Tenorio, Catalina Giraldo-Estrada
Summary: In this paper, the characteristics and chemical modification of pullulan produced by A. pullulans ATCC 15233 were studied. The pullulan derivative with a degree of substitution of 2.90 was synthesized through acetylation reaction, and its structure was elucidated using infrared and nuclear magnetic resonance spectroscopy. The results showed that pullulan exhibited typical glycosidic bonds and had a molecular weight of 93.47 kDa. Acetylation led to increased thermal stability and glass transition temperature. The pullulan film had homogeneous morphology and showed certain mechanical properties. The results suggest that biosynthesized pullulan and its derivatives have important physicochemical properties in the development of biomaterials.
Article
Multidisciplinary Sciences
WesamEldin I. A. Saber, Abeer A. Ghoniem, Fatimah O. Al-Otibi, Mohammed S. El-Hersh, Noha M. Eldadamony, Farid Menaa, Khaled M. Elattar
Summary: This study investigated the effects of three independent variables (tyrosine, sucrose, and incubation time) on melanin production by Aureobasidium pullulans AKW using response surface methodology and artificial neural network. The results showed that sucrose and incubation time significantly influenced melanin production, while tyrosine did not. The artificial neural network accurately predicted melanin production and achieved higher yields compared to response surface methodology.
SCIENTIFIC REPORTS
(2023)
Article
Agricultural Engineering
Ece Surek, Ali Oguz Buyukkileci, Sirma Yegin
Summary: A versatile process involving autohydrolysis and enzymatic hydrolysis was used to produce xylooligosaccharides (XOS) from hazelnut shells, with a maximum production of 22.5 g/L. The study demonstrated the potential of using A. pullulans xylanase for XOS production from agricultural waste materials.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Hetian Hu, Jeffrey M. Catchmark, Ali Demirci
Summary: The addition of pullulan significantly improved the mechanical properties and production of BC nanocomposites, while co-culture fermentation further enhanced the properties of BC.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Jong-Shik Kim, Dae-Won Ki, In-Kyoung Lee, Bong-Sik Yun
Summary: Biosurfactants are widely used in various industrial fields. The culture broth of Aureobasidium pullulans A11231-1-58 isolated from Chrysanthemum boreale Makino exhibited potent surfactant activity. Three new biosurfactants were isolated and their chemical structures were determined using spectroscopic techniques. The isolated compounds showed high degrees of surfactant activity.
JOURNAL OF ANTIBIOTICS
(2023)
Article
Multidisciplinary Sciences
Senthilkumar Preethy, Yoshitsugu Aoki, Katsura Minegishi, Masaru Iwasaki, Rajappa Senthilkumar, Samuel J. K. Abraham
Summary: In this study, a novel strain of Aureobasidium pullulans produced β-glucan (Neu-REFIX) was evaluated as a potential adjuvant therapy to slow down the progression of Duchenne muscular dystrophy (DMD) by exerting anti-inflammatory and anti-fibrotic effects. The results showed significant decreases in plasma liver enzyme levels and fibrosis area, as well as improvements in markers of inflammation and muscle damage in the N-163 β-glucan group.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Jong-Shik Kim, Dae-Won Ki, In-Kyoung Lee, Bong-Sik Yun
Summary: Biosurfactants are widely used in various industries, and the culture broth of Aureobasidium pullulans A11211-4-57 has potent surfactant activity. Two biosurfactants, myo-inositol lipids named pullusurfactans F and G, were isolated and showed high surfactant activity, making them suitable for washing and cleaning agents.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Microbiology
Difan Xiao, Lars M. Blank, Till Tiso
Summary: This article discusses the versatility of the black-yeast-like Aureobasidium as a cell factory for various biotechnological applications. It describes the draft genome sequence of Aureobasidium pullulans NRRL 62031, isolated in Thailand, which spans 25.05-Mb. The genome sequence reveals numerous synthesis pathways for valuable secondary metabolites.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Syahriar Nur Maulana Malik Ibrahim, Wichanee Bankeeree, Sehanat Prasongsuk, Hunsa Punnapayak, Pongtharin Lotrakul
Summary: The crude mannanase from Aureobasidium pullulans NRRL 58524 achieved maximum yield in the optimized medium with 3% defatted spent coffee grounds and 0.67% ammonium sulphate. The enzyme showed thermostability and maximum activity at 55 degrees C and pH 4.0. It also had limited catalytic activity on beta-glucan and was not affected by most ions and chelators. The potential of this enzyme in mannan hydrolysis and mannooligosaccharide production at a larger scale was demonstrated.
Article
Microbiology
Sashika M. Yalage Don, Leigh M. Schmidtke, Joanna M. Gambetta, Christopher C. Steel
Summary: The yeast-like fungus Aureobasidium pullulans produces volatile organic compounds (VOCs) with antifungal properties, which can trigger the accumulation of reactive oxygen species (ROS) and electrolyte leakage in microorganisms. Exposure to these VOCs alters cell wall structures of fungal mycelia and inhibits fungal growth.
RESEARCH IN MICROBIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Victor R. L. Oliveira, Victoria V. Coelho, Eliana F. C. Servulo, Veronica M. de A. Caladoa
Summary: The goal of this study was to investigate the sustainable development and characterization of pullulan, including its structure and rheological properties. The cultivation of A. pullulans strain and the utilization of industrial waste and low-cost renewable raw material were evaluated. The results showed that the pullulan obtained under specific fermentation conditions is suitable for the production of biopolymeric films in the future.
MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS
(2023)
Article
Medicine, Research & Experimental
Kadalraja Raghavan, Vidyasagar Devaprasad Dedeepiya, Vaddi Suryaprakash, Kosagi-Sharaf Rao, Nobunao Ikewaki, Tohru Sonoda, Gary A. Levy, Masaru Iwasaki, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel J. K. Abraham
Summary: This pilot clinical study demonstrated the beneficial effects of beta glucans derived from black yeast Aureobasidium pullulans on cytokine storm and coagulopathy biomarkers in COVID-19 patients. The combination of AFO-202 and N-163 beta glucans showed significant improvements in D-Dimer and IL-6 levels, as well as CD4+ and CD8+ T cell count. Larger multicentric clinical studies are recommended to further validate these findings for potential use in managing COVID-19 and long COVID-19 syndrome.
BIOMEDICINE & PHARMACOTHERAPY
(2022)