Article
Oncology
Mansheng Zhu, Qixiang Liang, Tao Chen, Qian Kong, Gengtai Ye, Shitong Yu, Xunjun Li, Qinglie He, Hao Liu, Yanfeng Hu, Jiang Yu, Guoxin Li
Summary: This study revealed the significant suppression of miRNA miR-129-2-3p in EBVaGC due to hypermethylation, and its role in promoting GC cell functions was demonstrated. The comprehensive analysis integrated multi-source data and provided insights into the relationships between methylated miRNA, differentially expressed genes (DEGs), and immune cell infiltration. Further experimental and clinical investigations are needed to validate these findings and explore the underlying mechanisms.
CLINICAL EPIGENETICS
(2021)
Article
Medicine, General & Internal
Yingsha Yao, Liujing Shi, Xiaoming Zhu
Summary: This study identified differential miRNAs in exosomes of endometrial cancer patients, four of which were closely related to clinical phenotypes and patient prognosis. These differential miRNAs may serve as prognostic biomarkers or therapeutic targets in endometrial cancer.
Review
Immunology
Reza Yarani, Ali Shojaeian, Oana Palasca, Nadezhda T. Doncheva, Lars Juhl Jensen, Jan Gorodkin, Flemming Pociot
Summary: This review discusses the differential regulation of miRNAs in inflammatory bowel disease (IBD) and their potential diagnostic and therapeutic applications. Through literature curation and computational analysis, a consensus set of differentially expressed miRNAs in mucosal tissues was obtained. The role of these miRNAs and their potential targets in IBD are highlighted.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Mohd Shuaib, Kumari Sunita Prajapati, Atul Kumar Singh, Shashank Kumar
Summary: In this study, novel miRNAs were identified in breast normal cells treated with Withaferin A and their expression patterns were studied in breast cancer cells. Bioinformatics tools and molecular dynamics simulations were used to analyze the secondary/tertiary structure of the identified miRNAs, target gene enrichment, miRNA-mRNA interaction, and stability. Hsa-miR-N88585 and hsa-miR-N461089 were identified as novel miRNAs in breast normal cells, showing overexpression in breast cancer cells and interaction with genes involved in important signaling pathways and GO terms. Molecular dynamics simulations indicated stable complex formation between hsa-miR-N88585/hsa-miR-N461089 and AGO protein. These findings suggest the potential role of these novel miRNAs in breast cancer pathophysiology.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Plant Sciences
Mohandas Snigdha, Duraisamy Prasath
Summary: This study analyzed the miRNAs and their target genes in ginger and mango ginger in response to R. solanacearum, finding higher enrichment of miRTGs in mango ginger than in ginger. Among the DEmiRTGs, 2105 were common in both ginger and mango ginger, while 2337 were unique to mango ginger, including 62 defense-related and upregulated miRTGs. Several key candidate miRNA/miRTGs pairs were identified in response to R. solanacearum infection in ginger, providing insights into the miRNA regulatory network in ginger and mango ginger in response to bacterial wilt.
Article
Biotechnology & Applied Microbiology
Li Yuan, Chunyu Zhang, Jiaying Li, Yuandong Liao, Hua Huang, Yuwen Pan, Qiqiao Du, Yili Chen, Wei Wang, Shuzhong Yao
Summary: In this study, circRNA array and mRNA-sequencing were used to profile the differentially expressed circRNAs and mRNAs in cervical cancer (CCa) tissues. The study identified hsa_circ_0026377 as downregulated in CCa and showed that its overexpression inhibited cell proliferation, migration, and invasion in CCa.
Article
Biotechnology & Applied Microbiology
Xiu-qin Hu, Han Song, Na Li, Chun-xiang Hao, Bo Zhang, Xin-peng Li, Jie Xin, Yong-qing Zhang
Summary: We sequenced miRNAs from male and female flower buds of the dioecious plant Trichosanthes kirilowii Maxim. using Illumina high-throughput sequencing technology. Through bioinformatics analysis and association analysis with previous transcriptome sequencing data, we identified 80 differentially expressed miRNAs between female and male plants. We also predicted target genes for these miRNAs and established a regulatory network, identifying 12 core genes involved in sex differentiation in TK.
Article
Veterinary Sciences
Eimear M. Donnellan, Jean-Philippe Perrier, Kate Keogh, Miriam Stiavnicka, Caitriona M. Collins, Elaine M. Dunleavy, Eli Sellem, Naomi C. Bernecic, Patrick Lonergan, David A. Kenny, Sean Fair
Summary: This study identified potential biomarkers for bull fertility by characterizing the spermatozoa transcriptome of high and low fertility bulls. The differentially expressed genes and miRNAs were related to embryonic development and gene expression regulation, providing new insights for assessing semen quality and bull fertility improvement.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Cell Biology
Xiaohui Tan, Shuyang Ren, Canyuan Yang, Shuchang Ren, Melinda Z. Fu, Amelia R. Goldstein, Xuelan Li, Leia Mitchell, Jill M. Krapf, Charles J. Macri, Andrew T. Goldstein, Sidney W. Fu
Summary: Lichen sclerosus (LS) is a chronic inflammatory skin disorder with unknown pathogenesis. Aberrant expression of microRNAs (miRNAs) is considered to play a crucial role in LS. This study identified a total of 170 differentially expressed miRNAs in female LS patients, with 119 upregulated and 51 downregulated, suggesting their potential importance in the pathogenesis of LS. Additionally, molecular network analysis provided further insight into the mechanisms underlying LS.
Article
Medicine, General & Internal
Fengfan Li, Chunlei Zhou, Shuxuan Li, Jingyu Wang, Ming Li, Hong Mu
Summary: Increasing evidence suggests that lncRNAs act as ceRNA in cancer, playing a key role in cancer initiation, invasion, and metastasis. This study constructed a lncRNA-miRNA-mRNA regulatory network and performed enrichment analysis to explore the potential mechanisms of lncRNAs in endometrial cancer (EC). Survival analysis revealed significant correlations between certain lncRNAs, miRNAs, and mRNAs and overall patient survival. This research provides new insights into the interactions among lncRNAs, miRNAs, and mRNAs and lays the foundation for further studies on the mechanisms of lncRNAs in EC occurrence.
Article
Genetics & Heredity
Yunan Su, Chaowei Li, Weifeng Liu, Yibin Liu, Liangyi Li, Qingshi Chen
Summary: This study investigated the expression profiles of miRNAs in a mouse model of chronic intermittent hypoxia-induced renal injury using miRNA sequencing. A total of 18 differentially expressed miRNAs were identified. The analysis also revealed the potential involvement of the Wnt signaling pathway in the development of this pathology.
FRONTIERS IN GENETICS
(2022)
Article
Medical Laboratory Technology
Zhi-jian Ren, Yang Zhao, Guan Wang, Le-le Miao, Zheng-chao Zhang, Long Ma, Mu-zhou Teng, Yu-min Li
Summary: This study identified differentially expressed microRNAs (DEMs) in serum exosomes between gastric cancer (GC) patients and healthy individuals, and predicted their target genes and pathways. Among them, three DEMs showed excellent diagnostic ability and eight target genes demonstrated perfect prognostic ability. The findings provide new targets for the diagnosis and treatment of GC.
CLINICA CHIMICA ACTA
(2022)
Article
Andrology
Gaozhao Liang, Qianqian Wang, Guohui Zhang, Zhongxiang Li, Qing Wang
Summary: Asthenozoospermia, characterized by low sperm motility, is found in 40% of infertile men. This study identified 13 dysregulated miRNAs in asthenospermia, including 5 previously characterized and 8 newly discovered miRNAs. These miRNAs are significantly associated with multiple signaling pathways, potentially leading to new technologies for diagnosis and prognosis of asthenospermia and new therapeutic options in personalized medicine.
Article
Genetics & Heredity
Weitao Hu, Taiyong Fang, Xiaoqing Chen
Summary: This study identified DEGs associated with ulcerative colitis (UC) using bioinformatics analysis, providing potential diagnostic and therapeutic targets. The DEGs, hub genes and miRNAs identified may serve as biomarkers for UC, but further research is needed to understand their biological roles in the disease.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Meng An, Xiaowen Zang, Jimin Wang, Jie Kang, Xiaoyu Tan, Bo Fu
Summary: This study identified 739 differentially expressed lncRNAs, 115 differentially expressed miRNAs, and 5749 differentially expressed mRNAs associated with breast cancer brain metastasis. Hub genes such as PTBP1, NUP98, and HYOU1 were found to be associated with BCBM. The lncRNA-miRNA-mRNA network revealed a potential mechanism of BCBM involving lncRNAs FIRRE and RP11-169F17.1 interacting with miR-501-5p to regulate gene expression.