期刊
JOURNAL OF ALZHEIMERS DISEASE
卷 54, 期 1, 页码 275-286出版社
IOS PRESS
DOI: 10.3233/JAD-160263
关键词
Alzheimer's disease; iTRAQ; mass spectrometry; oxygen; proteomics; water maze
资金
- National Natural Science Foundation of China [31470804]
- Key Programs of Shenzhen [JSGG20140703163838793]
- Basic Research Project of Shenzhen [20150006]
Background: Alzheimer's disease (AD) is a neurodegenerative disease with well-characterized pathological features. Yet the underlying mechanisms have not been resolved and an effective therapeutic approach is lacking. Cerebral hypoxia is considered a risk factor of AD. Objective: We tested whether oxygen supplementation can relieve AD symptoms and how it affects the expression levels of proteins in the lens. Methods: Triple transgenic AD model (3xTg-AD) mice were divided into oxygen treated (OT) and control (Ctrl) groups. Their cognitive performances were tested in a Morris water maze (MWM) paradigm. Then, their eye lens tissues were subjected to quantitative proteomics analysis by the iTRAQ (isobaric tags for relative and absolute quantification) method. The up-and downregulated proteins were classified according to a Gene Ontology (GO) database in PANTHER. Behavioral and proteomic data were compared between the groups. Results: Mice in the OT group had better learning and memorizing performance compared with the Ctrl group inMWMtest. Lenses from the OT group had 205 differentially regulated proteins, relative to lenses from the Ctrl group, including proteins that are involved in the clearance of amyloid beta-protein. Conclusion: The results of this study indicate that oxygen treatment can improve cognitive function in AD model mice and alters protein expression in a manner consistent with improved redox regulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据