Biodegradation performance and anti-fouling mechanism of an ICME/electro-biocarriers-MBR system in livestock wastewater (antibiotic-containing) treatment

Livestock wastewater is an important reservoir of antibiotic resistance genes (ARGs) and antibiotic residues. Membrane fouling is one of the most challenging problems confining the operation and application of membrane bioreactor (MBR). In this work, a novel iron-carbon micro-electrolysis (ICME)/electro-biocarriers-MBR system was established to explore the performance of pollutant removal and anti-fouling for an actual livestock wastewater. A light-weight porous ceramsite (bulk density 0.98 g/cm(3)) was used as the MBR biocarriers. The electrons generated from iron corrosion in the ICME tank traveled through external wires to the stainless steel membrane modules of MBR and the protons were transferred from the MBR tank to the ICME tank through a salt bridge, thus producing a spontaneous electric field. Under the optimized conditions, the system exhibited chemical oxygen demand removal of 76.0%, total suspended solids removal of 100%, antibiotic removal of 86.4%, NH4+ -N removal of 91.1%, and ARGs reduction of 6-8 orders of magnitude. The quality of the final effluent can reach the national Class I-A discharge criteria. Adding ceramsite could not only effectively improve biodegradation performance but also alleviate membrane fouling through the migration and enrichment of microbial flora to the ceramsite. The self-generated electric field had no significant improvement effect on pollutant removal, but exhibited good anti-membrane fouling behavior which could be ascribed to (i) oxidization of membrane foulants by the electrochemical products (such as H2O2 and center dot OH radicals), and (ii) electrostatic repulsion of negatively charged foulants and bacterial cells. The bacterial community structure and diversity were studied using high-throughput pyrosequencing, and the results demonstrated the roles of electric field and biocarriers in enrichment of anti-fouling communities and repulsion of biofouling-creating communities.

Automated summary

The novel iron-carbon micro-electrolysis (ICME)/electro-biocarriers-MBR system was effective in removing pollutants from actual livestock wastewater and reducing the concentration of ammonia nitrogen and antibiotic resistance genes (ARGs). Additionally, the system exhibited good anti-membrane fouling behavior, which could be attributed to the oxidization of membrane foulants by electrochemical products and electrostatic repulsion of foulants and bacterial cells.