Article
Biotechnology & Applied Microbiology
Hao Yang, Zhenfei Wang, Shujun Shi, Qin Yu, Meiling Liu, Zhelin Zhang
Summary: This study demonstrated that CSF metabolomics based on LC-MS can differentiate neurobrucellosis patients from normal controls, offering new perspectives for diagnosis and treatment of neurobrucellosis.
Article
Multidisciplinary Sciences
Lingfei Hu, Zhenwei Wu, Christelle A. M. Robert, Xiao Ouyang, Tobias Zust, Adrien Mestrot, Jianming Xu, Matthias Erb
Summary: Plant secondary metabolites play a dual role in plant defense and root interactions with herbivores, with soil iron levels influencing the impact of these metabolites on herbivore growth. This study highlights the complex interplay between soil chemistry and plant defense mechanisms, demonstrating the multifunctionality of plant secondary metabolites in shaping interactions in variable environments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Fei-Long Liu, Tian-Tian Ye, Jiang-Hui Ding, Xiao-Ming Yin, Xiao-Ke Yang, Wei-Hua Huang, Bi-Feng Yuan, Yu-Qi Feng
Summary: Researchers synthesized paired reagents of DMPI and d(3)-DMPI, which can efficiently react with phosphate groups on polar phosphorylated metabolites. They found that tagging DMPI onto ribonucleotides significantly increased ionization efficiencies during LC/MS analysis, resulting in enhanced detection sensitivities.
ANALYTICAL CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Jun Kou, Chunyang He, Lin Cui, Zhengping Zhang, Wei Wang, Li Tan, Da Liu, Wei Zheng, Wei Gu, Ning Xia
Summary: The study on Chinese postmenopausal women with osteoporosis identified potential biomarkers related to metabolic pathway disorders, providing new insights for early diagnosis. The findings suggest that metabonomic analysis has great potential for application in postmenopausal women with osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Food Science & Technology
Dhara Dixit
Summary: This study reveals the biochemical traits, mineral makeup, and secondary metabolite outline of Iyengaria stellata using untargeted metabolite profiling. A total of 108 putative metabolites were detected, including steroids, terpenoids, ketones, esters, polyphenols, anthraquinones, tocopherol, etc. The study highlights the potential of I. stellata as a natural source in pharmaceutical and nutraceutical compositions.
Article
Biochemistry & Molecular Biology
Adamantios Krokos, Olga Deda, Christina Virgiliou, Helen Gika, Nikolaos Raikos, Eleni Aggelidou, Aristeidis Kritis, Georgios Theodoridis
Summary: This study evaluated the effect of cocaine on human liver cancer cell lines and revealed the predominant impact of cocaine on the metabolic pathways of alanine, aspartate, and glutamate. Targeted metabolomics identified changes in metabolites upon cocaine administration, but deciphering the exact cocaine cytotoxic mechanism remains challenging.
Article
Medicine, General & Internal
Stefano Angioni, Francesca Congiu, Salvatore Giovanni Vitale, Maurizio Nicola D'Alterio, Antonio Noto, Giovanni Monni, Maria Laura Santoru, Vassilios Fanos, Federica Murgia, Luigi Atzori
Summary: This study used gas chromatography-mass spectrometer (GC-MS) to analyze serum samples of patients with symptomatic endometriosis and found metabolic alterations and potential biomarkers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Chemistry, Analytical
Teresa S. McMillen, Andrew Leslie, Kenneth Chisholm, Susanne Penny, Jeffrey Gallant, Alejandro Cohen, Arik Drucker, James P. Fawcett, Devanand M. Pinto
Summary: This study presents a comprehensive and quantitatively robust method for metabolomic analysis, using HILIC and RP stationary phases combined with positive and negative electrospray ionization. The method allows for complete analysis of a single sample in as little as 40 minutes and has been validated with clinical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Endocrinology & Metabolism
Qingying Si, Jinxiu Guo, Xiumei Yang, Yujin Guo, Linlin Wu, Dadi Xie, Pei Jiang
Summary: This study investigated the metabolic alterations in streptozotocin-induced diabetic rats using gas chromatography-mass spectrometry analysis. The results showed differential expression of metabolites in the serum, heart, liver, kidney, cortex, renal lipid, hippocampus, and brown fat tissues of diabetic rats compared to control rats. Metabolic pathway analysis revealed that these alterations mainly involved amino acid metabolism, organic acid metabolism, sugar metabolism, and lipid metabolism.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Plant Sciences
Richard P. Jacoby, Anna Koprivova, Stanislav Kopriva
Summary: Plants use a variety of secondary metabolites to influence the composition and function of the microbiome, which can be enhanced through targeted methods to improve beneficial microbial traits and enhance agricultural sustainability.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Wen-Feng Li, Shuang Li, Jie Liu, Xiao-Fei Wang, Hui-Yun Chen, Hua Hao, Ke-Jian Wang
Summary: The study found that during the pubertal molt of Scylla paramamosain, carbohydrates in the serum decreased, indicating that free sugars were used for energy supply and there was insufficient glucose supply; at the same time, the metabolic pathways of fatty acyls were significantly affected, the carnitine shuttle was stimulated, and beta-oxidation was upregulated.
Article
Agriculture, Multidisciplinary
Brigitte Heike Schwendel, Praju Vikas Anekal, Erica Zarate, Kyung Whan Bang, George Guo, Angus C. Grey, Farhana R. Pinu
Summary: The study utilized a metabolomics approach to investigate the biochemical changes in grape berries due to mechanical shaking, revealing significant alterations in primary and secondary metabolism post shaking. Accumulation of specific metabolites near the skin of shaken berries may be associated with the formation of a protective barrier against Botrytis cinerea infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Ecology
Moriah Sandy, Tina I. Bui, Kenia Segura Aba, Nestor Ruiz, John Paszalek, Elise W. Connor, Christine V. Hawkes
Summary: The chemical interactions between fungal endophytes and plant leaves are likely to be the key mechanism affecting plant phenotypic traits. This study found that multiple fungal strains share functional similarities in their effects on plant growth and physiology under unstressed conditions. Plant metabolomics profiles were correlated with fungal treatments, and specific metabolites were associated with plant trait variation. These findings have implications for the development of metabolites as crop management tools.
Article
Cell Biology
Andrzej Patyra, Marta Katarzyna Dudek, Anna Karolina Kiss
Summary: This study analyzed and compared the chemical profiles of wood extracts from different species in the Pinaceae family. The results showed that different species contained a variety of compounds, with Norway spruce being rich in stilbenes, European larch mostly containing flavonoids, and silver fir rich in lignans. The findings also suggested that conifer branches, a waste material from the wood industry, could be a valuable source of phytochemicals.
Article
Environmental Sciences
Kapil Mandrah, Veena Jain, Shagun Shukla, Jamal Ahmad Ansari, Pankaj Jagdale, Anjaneya Ayanur, Vikas Srivastava, Somendu Kumar Roy
Summary: This study investigated the nephrotoxic effects of bisphenol S (BPS) exposure. Results showed that BPS caused histopathological disturbance in the kidney and altered the levels of kidney damage biomarkers in serum and urine samples. Additionally, BPS perturbed the metabolic pathways of the kidney.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Chemistry, Applied
Mohamed Shaaban, Ahmed S. Abdel-Razek, Viola Previtali, Mads Hartvig Clausen, Charlotte H. Gotfredsen, Hartmut Laatsch, Ling Ding
Summary: In this study, a fungal endophyte, Aspergillus sp. strain FVL2, isolated from the traditional medicinal fennel plant, was investigated for its secondary metabolites. Three new natural products, along with 14 known metabolites, were discovered through fermentation and chromatographic separation. The chemical structures of the new metabolites were determined using NMR spectroscopy and ESI HR mass spectrometry. This discovery of diverse secondary metabolites expands the chemical space of Aspergillus.
NATURAL PRODUCT RESEARCH
(2023)
Article
Physics, Atomic, Molecular & Chemical
Bina Bhattarai, Stine K. Steffensen, Dan Staerk, Bente B. Laursen, Inge S. Fomsgaard
Summary: This study presents a mass spectrometry-based method to locate and annotate BX-conjugates in plant extracts, allowing for the identification of previously unknown BXs in the roots of a dicotyledonous plant. These BXs may contribute to the biological activities of the plant.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Multidisciplinary Sciences
Elise Nordin, Stine K. Steffensen, Bente B. Laursen, Sven-Olof Andersson, Jan-Erik Johansson, Per Aman, Goran Hallmans, Michael Borre, Dan Staerk, Kati Hanhineva, Inge S. Fomsgaard, Rikard Landberg
Summary: In this study, a quantitative method for analysis of benzoxazinoids (BXs) was established, and the plasma levels of BXs after consumption of whole grain/bran rye or refined wheat were investigated in men with prostate cancer. The results showed that BX metabolites were significantly higher after rye consumption compared to refined wheat. Additionally, four BX-related metabolites were inversely associated with PSA levels in plasma. These findings suggest that rye may have potential preventive effects against prostate cancer.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Sheng-Da Zhang, Laura Louise Lindqvist, Thomas Isbrandt, Ingrid Lykke Borre, Mario Wibowo, Maike Wennekers Nielsen, Ling Ding, Thomas Ostenfeld Larsen, Lone Gram
Summary: The biosynthetic genetic basis and pathway of solonamides in P. galatheae S2753 were revealed, which may lead to the industrial production of solonamides. The comparison between a solonamide-deficient mutant and wild-type S2753 demonstrated that solonamides stimulate the swimming behavior of S2753 and influence a few key physiological processes of the native producers. These compounds not only serve as novel drug candidates but also provide competitive benefits to the native producer for survival in nature.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stella Scholz, Sofia Kerestetzopoulou, Vincent Wiebach, Romina Schnegotzki, Bianca Schmid, Emmanuel Reyna-Gonzalez, Ling Ding, Roderich D. Sussmuth, Elke Dittmann, Martin Baunach
Summary: In this study, a chemoenzymatic in vitro platform was used to introduce functional tags in various microviridin variants, resulting in biotinylated, dansylated, or propargylated congeners. This direct approach provides a pathway for the development of customized protease inhibitors with built-in functionalities.
Article
Agronomy
Isaac Kwesi Abuley, Hans Albert Pedersen, Jonas Duus Stevens Lekfeldt, Inge Sindbjerg Fomsgaard, Sabine Ravnskov
Summary: This study aimed to characterize the effects of two P. infestans isolates and host resistance on secondary metabolite expression in potato plants. The results reveal important differences in the role of secondary metabolism in resistance to P. infestans.
Review
Agronomy
Mahnaz Mirzaei, Eskandar Zand, Mehdi Rastgoo, Alireza Hasanfard, Per Kudsk
Summary: Water quality parameters such as hardness, pH, alkalinity, turbidity, and TDS play a role in the effectiveness of herbicides in spray applications. Hard water with polyvalent cations can decrease the efficacy of weak acid post-emergence herbicides. Different approaches, such as water conditioning adjuvants containing nitrogen compounds like ammonium sulfate (AMS), ethoxylated amine surfactants, organic acids, and EDTA, have been proposed to overcome the negative effects of poor water quality. Understanding the impact of water properties on herbicide performance is crucial for optimizing herbicide use and avoiding excessive use, especially in regions with poor water quality.
Article
Biochemistry & Molecular Biology
Samuel A. Bradley, Beata J. Lehka, Frederik G. Hansson, Khem B. Adhikari, Daniela Rago, Paulina Rubaszka, Ahmad K. Haidar, Ling Chen, Lea G. Hansen, Olga Gudich, Konstantina Giannakou, Bettina Lengger, Ryan T. Gill, Yoko Nakamura, Thomas Duge de Bernonville, Konstantinos Koudounas, David Romero-Suarez, Ling Ding, Yijun Qiao, Thomas M. Frimurer, Anja A. Petersen, Sebastien Besseau, Sandeep Kumar, Nicolas Gautron, Celine Melin, Jillian Marc, Remi Jeanneau, Sarah E. O'Connor, Vincent Courdavault, Jay D. Keasling, Jie Zhang, Michael K. Jensen
Summary: This study reports a yeast platform for the de novo biosynthesis of medicinal plant compounds. Using refactored plant pathways, the researchers successfully synthesized 19 halogenated derivatives with therapeutic potential.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Plant Sciences
Ida K. L. Andersen, Lars O. Dragsted, Jim Rasmussen, Inge S. Fomsgaard
Summary: Intercropping barley affects the metabolome of lupin, leading to increased levels of flavonoids and decreased levels of tryptophan. These flavonoids may play a role in regulating lupin's nitrogen fixation activity.
JOURNAL OF PLANT INTERACTIONS
(2023)
Article
Environmental Sciences
Robert Finger, Niklas Mohring, Per Kudsk
Summary: Glyphosate, the most widely used pesticide in Europe, is under discussion for renewal due to its potential effects on human health. This study provides a literature review synthesizing the existing evidence on the potential economic impacts of a glyphosate ban on European agriculture. The findings reveal that a ban on glyphosate can have significant economic implications, particularly for high-value perennial crops like fruits and grapevines. However, the range of impacts reported in the literature is wide, and there is still a lack of comprehensive assessments for most countries and farming systems in Europe.
COMMUNICATIONS EARTH & ENVIRONMENT
(2023)
Article
Horticulture
Tabata Bergonci, Inge S. S. Fomsgaard, Katrine H. H. Kjaer, Ivan A. A. Paponov
Summary: In horticulture and floriculture, the success of vegetative propagation in plants depends on their capacity to form adventitious roots at the basal part of stem cuttings. This study investigated the role of flavonoids and plant hormones in AR formation in two different genotypes of Campanula portenschlagiana. The blue-flowered genotype showed higher accumulation of flavonols and flavones in petals, as well as in the basal part of cuttings, leading to better AR formation compared to the white-flowered genotype. The improved AR formation was associated with higher auxin levels and lower ABA levels in the basal part of the cuttings.
Article
Plant Sciences
S. W. Wilkinson, A. Hannan Parker, A. Muench, R. S. Wilson, K. Hooshmand, M. A. Henderson, E. K. Moffat, P. S. C. F. Rocha, H. Hipperson, J. H. M. Stassen, A. Lopez Sanchez, I. S. Fomsgaard, P. Krokene, M. H. Mageroy, J. Ton
Summary: Stress can have long-lasting effects on plants. The stress hormone jasmonic acid (JA) can induce resistance to herbivores but increase susceptibility to pathogens in Arabidopsis. JA treatment leads to long-term priming and upregulation of JA-dependent defense genes while repressing ethylene- and salicylic acid-dependent genes.
Meeting Abstract
Plant Sciences
O. Tsiftsoglou, M. S. Cowled, L. Ding