期刊
CANCER BIOLOGY & THERAPY
卷 16, 期 9, 页码 1364-1374出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15384047.2015.1070988
关键词
adenosine receptor; breast cancer; cholera toxin; G protein-coupled receptor; protein isoprenylation; protein kinase A (PKA); Ras-related protein 1 (Rap1); beta-adrenergic receptor
类别
资金
- NIH [R01 CA136799, R01 CA188871]
- Rock River Cancer Research Foundation
- WBCS Inc.
- Nancy Laning Sobczak, Ph. D., Breast Cancer Research Award
- NIGMS [T32-GM080202]
A greater understanding of the molecular basis of breast cancer metastasis will lead to identification of novel therapeutic targets and better treatments. Rap1B is a small GTPase that suppresses the metastasis of breast cancer cells by increasing cell-cell adhesion. In breast cancer, a decrease in Rap1B prenylation and subsequent loss of Rap1B at the plasma membrane decreases cell-cell adhesion and increases cell scattering, which promotes the metastatic phenotype. Protein kinase A (PKA) was recently found to phosphorylate Rap1B and inhibit its prenylation. PKA is activated by G protein-coupled receptors (GPCR) that stimulate G(s). In this study, we investigated whether the general G(s) activator, cholera toxin, and agonists of the -adrenergic receptor (AR), which is a G(s)-coupled GPCR, promote Rap1B phosphorylation and inhibit its prenylation. We show here that cholera toxin and AR activation phosphorylate Rap1B and inhibit its prenylation and membrane localization, reducing cell-cell adhesion and promoting cell scattering. Furthermore, we report that breast cancer cell migration is decreased by the FDA-approved -blocker, propranolol. Pharmacological targeting of GPCRs, especially those such as the AR that are regulated by FDA-approved drugs, to increase cell adhesion and decrease cell scattering could provide a promising therapeutic approach to reduce breast cancer metastasis.
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