Review
Cell Biology
Xingde Wang, Xingzhi Xu, Zhifeng Wang
Summary: In this review, the authors summarize the known information about the UFM1 enzymatic cascade and its biological functions, discuss the identified substrates, and explore the pathological role of UFMylation in human disease.
Review
Veterinary Sciences
Tong Zhou, Mingshu Wang, Anchun Cheng, Qiao Yang, Bin Tian, Ying Wu, Renyong Jia, Shun Chen, Mafeng Liu, Xin-Xin Zhao, Xuming Ou, Sai Mao, Di Sun, Shaqiu Zhang, Dekang Zhu, Juan Huang, Qun Gao, Yanling Yu, Ling Zhang
Summary: This article mainly describes the viral protein kinases and their mechanisms of regulating viral protein function through phosphorylation. The study of post-translational modification of viral proteins is of great significance for understanding viral infection mechanisms and developing antiviral treatment.
VETERINARY RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Lynsay Blake, Martin J. Cann
Summary: Carbon dioxide plays a crucial role in various life processes, regulating cellular reactions, transport, maintenance, and behavior. Protein carbamate modification, mediated by carbon dioxide, is a mechanism that may alter protein function and participate in sensing and signaling.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Xuan Li, Kai-Bin Yang, Wei Chen, Jia Mai, Xiao-Qi Wu, Ting Sun, Rui-Yan Wu, Lin Jiao, Dan-Dan Li, Jiao Ji, Hai-Liang Zhang, Yan Yu, Yu-Hong Chen, Gong-Kan Feng, Rong Deng, Jun-Dong Li, Xiao-Feng Zhu
Summary: The E3 ubiquitin ligase CUL3 interacts with BECN1 to promote its ubiquitination and degradation, leading to a decrease in autophagic activity and promoting tumor cell proliferation. Additionally, KLHL38 acts as a substrate adaptor for the CUL3 E3 ligase complex-mediated degradation of BECN1.
Article
Chemistry, Physical
Lucas W. Henderson, Edie M. Sharon, Amit K. S. Gautam, Adam J. Anthony, Martin F. Jarrold, David H. Russell, Andreas Matouschek, David E. Clemmer
Summary: Mass spectrometry studies show that the stability of S. cerevisiae 20S proteasome undergoes related configurations and transitions during temperature changes, possibly linked to the opening of the proteolytic core. The study indicates that the proteasome remains intact and all transitions are reversible. Three types of structures are identified based on thermodynamic analysis: energetically stabilized closed configurations, high-entropy precursor states, and open pore structures. Opening of the 20S pore in the absence of the regulatory unit involves a charge-priming process. However, only a small fraction of precursor configurations actually open to expose the catalytic cavity.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Review
Biochemistry & Molecular Biology
Stefania Pieroni, Marilena Castelli, Danilo Piobbico, Simona Ferracchiato, Damiano Scopetti, Nicola Di-Iacovo, Maria Agnese Della-Fazia, Giuseppe Servillo
Summary: A cancer outcome is a multifactorial event that arises from both external injuries and internal predisposing factors. Protein synthesis, controlled by post-translational modifications, plays a crucial role in cellular function regulation. The correct activity of post-translational machinery is essential to prevent the development of cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Qibin Wu, Zhenxiang Li, Jingtao Yang, Fu Xu, Xueqin Fu, Liping Xu, Chuihuai You, Dongjiao Wang, Yachun Su, Youxiong Que
Summary: This study reports the first comprehensive analysis of protein lysine acetylation, 2-hydroxyisobutyrylation, and lysine lactylation in sugarcane. These post-translational modifications were found to be involved in energy metabolism and stress response. The results provide new insights into the molecular mechanisms of protein PTMs in sugarcane.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Oncology
Jie Wang, Yuandi Xiang, Mengqi Fan, Shizhen Fang, Qingquan Hua
Summary: The reprogramming of tumor cell metabolism is a key characteristic of cancer, providing energy and building blocks for tumor development. The ubiquitin-proteasome system (UPS) is involved in tumor metabolism. This review highlights the role of UPS in major metabolic enzymes and signaling pathways, and provides an overview of small molecules and drugs in clinical trials. Understanding the role of ubiquitination and deubiquitination in tumor metabolism may lead to new strategies for cancer treatment.
Article
Biochemistry & Molecular Biology
Meredith Juncker, Catherine Kim, Ryan Reed, Arthur Haas, Joshua Schwartzenburg, Shyamal Desai
Summary: The study reveals defective mitophagy in neurodegenerative diseases such as Ataxia Telangiectasia and Amyotrophic Lateral Sclerosis, which is associated with constitutively elevated ISG15 pathway. Inhibition of the ISG15 pathway restores mitophagy, reduces oxidative stress, and unhealthy mitochondria levels, suggesting a potential therapeutic avenue for neurodegenerative disorders.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Xiao Yang, Meihui Song, Ying Wang, Taicong Tan, Zhongyu Tian, Binyuan Zhai, Xuan Yang, Yingjin Tan, Yanding Cao, Shaojun Dai, Shunxin Wang, Liangran Zhang
Summary: This study identifies a molecular pathway for axis length regulation in meiotic cells. It shows that the cohesin regulator Pds5 interacts with proteasomes to regulate the ubiquitination level of chromosome proteins, which affects chromosome axis length. Two ubiquitin E3 ligases, SCF and Ufd4, are involved in this pathway. These findings contribute to our understanding of how Pds5 regulates meiotic chromosome organization and suggest a conserved regulatory mechanism in higher eukaryotes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Plant Sciences
Dania P. Lobaina, Roberto Tarazi, Tamara Castorino, Maite F. S. Vaslin
Summary: The ubiquitin-proteasome system (UPS) is crucial for maintaining cellular balance and plays a key role in DNA repair, gene transcription, and protein activation. Viruses often manipulate the UPS to maintain viral protein levels and evade host defenses. Understanding the mechanisms used by plant viruses to subvert host defenses is important for developing biotechnological approaches for viral resistance.
Article
Chemistry, Multidisciplinary
Buyan Pan, Marie Shimogawa, Jun Zhao, Elizabeth Rhoades, Anna Kashina, E. James Petersson
Summary: In this study, a facile method for introducing a mimic of arginylation modification into a protein of interest is presented. The effects of this mimicking modification on protein aggregation and neuroprotection are investigated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Marta Pichlak, Tomasz Sobierajski, Katarzyna M. Blazewska, Edyta Gendaszewska-Darmach
Summary: PROTACs are a promising field in medicinal chemistry, with compounds that have reached clinical studies. They target posttranslational modifications and have shown significant progress in targeting kinases. This study focuses on a less popular class of PROTACs that target enzymes involved in lysine acetylation/deacetylation, lysine and arginine methylation, ADP-ribosylation, E3 ligases, and ubiquitin-specific proteases. The authors emphasize the importance of understanding the structural aspects of PROTAC elements to aid in the development of these compounds.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Karlie R. Platz, Emma J. Rudisel, Katelynn V. Paluch, Taylor R. Laurin, Kristin E. Dittenhafer-Reed
Summary: The mitochondrial proteome undergoes various post-translational modifications, and this study focused on the effects of acetylation and phosphorylation on the function of mitochondrial RNA polymerase (POLRMT). While some modifications showed slight decrease in the binding ability of POLRMT, there were minimal biological impacts observed in terms of viability, mtDNA content, and mitochondrial transcript levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Jae-Ho Park, Changwon Kho
Summary: Calcium signaling in the heart is crucial for muscle contraction and electrical signals, with impairment of Ca2+ handling proteins being a key hallmark of heart disease. The discovery of miRNAs as gene regulators has expanded our understanding of cardiac Ca2+ cycling, and there has been significant exploration of their involvement in heart diseases. This review aims to summarize Ca2+ signaling and Ca2+-related miRNAs in cardiac pathologies, as well as discuss the therapeutic potential of targeting these miRNAs for heart disease treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ahyoung Lee, Jiwon Moon, Jin Yu, Changwon Kho
Summary: This review discusses the clinical significance of microRNAs as potential biomarkers and therapeutic targets for DMD and DMD cardiomyopathy as examples of dystrophinopathies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
