Article
Biochemical Research Methods
Wenwei Wu, Qifeng Sun, Tiansong Li, Kexin Liu, Yan Jiang, Yi Wang, Yongjie Yang
Summary: The bispecific aptamers against MG and LMG were isolated using Capture-SELEX, and their specificity and affinity were confirmed. This study provides novel probes for the development of multi-residues rapid detection and opportunities for multiple target aptamer discovery.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Qingqing He, Mian Wang, Yajie Zhao, Guiyu Tan, Man Zhang, Rui Feng, Yujie Chen, Baomin Wang, Qing X. Li
Summary: To improve the selection efficiency of nanobodies against atrazine in a phage display library, anti-carrier protein phages were depleted using immunomagnetic beads conjugated with bovine serum albumin. This depletion strategy tripled the number of atrazine-positive phage clones and resulted in the selection of a sensitive phage clone with greater thermostability and organic solvent tolerance compared to a monoclonal antibody. The Nb3-based immunoassay showed similar specificity but higher IC50 and method detection limit compared to the mAb 5D9-based immunoassay.
ANALYTICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Wanyao Song, Mengyu Luo, Huaming Li, Jiaxu Xiao, Xiuping He, Jixiang Liang, Dapeng Peng
Summary: In this study, an ic-ELISA method was developed using a new generation metabolite (DMEQ)-based monoclonal antibody (mAb) for the rapid detection of Qx residues in food. The method exhibited high sensitivity and a wide linear range, and showed good cross-reactivity with multiple DQx. Furthermore, the method demonstrated good correlation with LC-MS/MS results in animal-derived foods, indicating its potential for rapid screening of Qx residues.
Article
Chemistry, Applied
Cheng Liang, Yuxiang Ji, Jiyong Ma, Chundong Zhang, Hongwei Zhao
Summary: The study reported a highly sensitive monoclonal antibody against okadaic acid (OA) and developed a more sensitive indirect competitive ELISA (icELISA) for rapid detection of OA.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2022)
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Article
Environmental Sciences
Yu-Ru Lin, Yeh-Fang Hu, Chih-Yang Huang, Huai-Ting Huang, Zhen-Hao Liao, An-Ting Lee, Yu-Sheng Wu, Fan-Hua Nan
Summary: Zeolite, oyster shell powder (OSP), and humic acid (HA) showed high affinity for malachite green (MG) and its metabolite leucomalachite green (LMG) in both freshwater and seawater, while Chloride dioxide (ClO2) exhibited greater efficiency for MG and LMG degradation. These water treatment agents have the potential to reduce the amount of hazardous MG and LMG residue in aquatic environments.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Chemistry, Analytical
Guangxin Yang, Jingru Zhang, Lin Gu, Yunyu Tang, Xuan Zhang, Xuanyun Huang, Xiaosheng Shen, Wenlei Zhai, Essy Kouadio Fodjo, Cong Kong
Summary: A novel ratiometric fluorescence immunoassay was developed for monitoring the illegal use of malachite green (MG) in aquaculture. The method showed a linear ratiometric fluorescence response for MG with a limit of detection of 0.097 mu g center dot kg(-1) and recoveries ranging from 81.88 to 108%. It offers rapid detection, simple sample preparation, and stable signal readout.
Article
Environmental Sciences
Min -ling Liu, Zi-jian Chen, Xiao-qing Huang, Hong Wang, Jin-li Zhao, Yu-dong Shen, Lin Luo, Xiao-wei Wen, Bruce Hammock, Zhen-lin Xu
Summary: This study provides a bispecific nanobody (BsNb) with high sensitivity and efficiency against environmental pesticide and its metabolite, and explores the influence of linker on recognition using molecular dynamics simulation for the first time. The results are valuable for the application of high-efficiency immunoassay in the fields of environment and agriculture.
ENVIRONMENTAL POLLUTION
(2023)
Article
Food Science & Technology
Disha Lu, Xu Wang, Ruijue Su, Yongjian Cheng, Hong Wang, Lin Luo, Zhili Xiao
Summary: A novel and efficient immunoaffinity column based on bispecific monoclonal antibody recognizing AFB(1) and OTA was developed and successfully applied in the simultaneous extraction and detection of AFB(1) and OTA from food samples.
Article
Chemistry, Applied
Supaluk Krittanai, Piriyakorn Pichetpongtorn, Seiichi Sakamoto, Waraporn Putalun
Summary: Licochalcone A (LicoA), a compound found in Chinese licorice, has been approved for the treatment of rosacea and acne due to its antimicrobial and anti-inflammatory properties. A monoclonal antibody against LicoA was developed using a mannich reaction hapten conjugate and hybridoma technique. An ELISA method was then developed to quantitatively determine the concentration of LicoA. This method showed high specificity and can be used for quality control of licorice and its derived products.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Rongzhi Wang, Juncheng Wang, Haimei Liu, Yehong Gao, Qiang Zhao, Sumei Ling, Shihua Wang
Summary: The study identified a specific IgG against bovine LF for rapid detection of LF content in cow milk using ic-ELISA and lateral flow immunosensors, without the need for expensive equipment and sample pretreatment.
Article
Chemistry, Analytical
Huihui Wu, Xinxin Xu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu
Summary: A highly specific and sensitive monoclonal antibody against quinclorac was prepared in this study, and an ic-ELISA and an immunochromatographic strip assay were established for Qui detection. The analysis results were consistent with LC-MS/MS, indicating that the established methods can provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples.
Article
Biochemical Research Methods
Worapol Sae-Foo, Sukritta Singkham, Pimsiri Srisongkhram, Gorawit Yusakul, Pisitchai Masugarut, Waraporn Putalun
Summary: In this study, immunoassays utilizing specific anti-GTG monoclonal antibodies were successfully developed, including a lateral-flow immunoassay for rapid GTG detection and an icELISA for GTG quantification. The results showed that these methods exhibited high specificity and accuracy.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Shaozhen Li, Jianping Zhu, Wei Ma, Hua Kuang, Liqiang Liu, Chuanlai Xu
Summary: This study developed a highly sensitive monoclonal antibody for colchicine detection, and prepared a lateral-flow immunochromatographic assay strip for on-site detection of colchicine in food samples.
ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Kun Peng, Ketevan Siradze, Saloumeh Kadkhodayan Fischer
Summary: By characterizing the immunogenic domain(s) of the bispecific monoclonal antibody Anti-A/B and implementing a monitoring plan, this study provided insights into immunogenicity results and potential clinical impacts, which can be generalized to other molecules with multiple-binding domains.
