Article
Medicine, General & Internal
Esra'a Abudouleh, Fatimah Alhamlan, Arwa A. Al-Qahtani, Marie Fe Bohol, Amal Al Hazzani, Khadija Khorfan, Morad Alkaff, Tarek Owaidah, Ahmed A. Al-Qahtani
Summary: This study investigated coagulation and fibrinolysis parameters in COVID-19 patients and their association with disease severity. The results showed significantly elevated levels of thrombin activatable fibrinolysis inhibitor, fibrinogen, and tissue plasminogen activator in severe patients. Additionally, patients in the ICU had significantly higher levels of plasminogen activator inhibitor-1, tissue plasminogen activator, and thrombin activatable fibrinolysis inhibitor. These coagulation markers can serve as suitable prognostic indicators for hypofibrinolysis.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Max Liu, Khalequz Zaman, Yolanda M. Fortenberry
Summary: Aptamers are single-stranded DNA or RNA sequences that exhibit high specificity and affinity in binding target molecules. They are non-immunogenic, easier to synthesize and modify, and can bind targets with greater affinity, making them a promising therapeutic candidate for treating various conditions. Active research is ongoing in developing aptamers to target blood coagulation factors, with potential applications in treating cardiovascular diseases, blood disorders, and cancers. Although no aptamers targeting blood coagulation factors have been approved for clinical use, several have shown promise in clinical trials and preclinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Claire S. Whyte, Hadj Ahmed. Mostefai, Kim M. Baeten, Andrew J. Lucking, David E. Newby, Nuala A. Booth, Nicola J. Mutch
Summary: The resolution of arterial thrombi relies on the endogenous fibrinolytic system. tPA primarily localizes to the thrombus head in a free and active form. Thrombi formed under high shear conditions are more resistant to fibrinolysis due to less tPA and increased PAI-1 concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Environmental Sciences
Kai Wang, Wanzhou Wang, Lei Lei, Yang Lan, Qisijing Liu, Lihua Ren, Shaowei Wu
Summary: This study conducted a systematic review and meta-analysis of existing epidemiological research to examine the associations between ambient air pollution and coagulation biomarkers. The results showed that short-term exposures to PM2.5 and O3 were associated with significant increases in coagulation biomarkers.
ENVIRONMENTAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Kirstin Meiners, Prisca Hamm, Marcus Gutmann, Jan Niedens, Agnieszka Nowak-Krol, Salvador Pane, Tessa Luehmann
Summary: The study developed three polyethylene glycol (PEG)ylated tPA bioconjugates based on the recombinant human tPA drug Alteplase using site-specific conjugation strategies. These bioconjugates showed improved proteolytic activity, slower inhibition kinetics by PAI-1, and increased hydrodynamic size.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Maria D. Valls, Maria Soldado, Jorge Arasa, Miguel Perez-Aso, Adrienne J. Williams, Bruce N. Cronstein, M. Antonia Noguera, M. Carmen Terencio, M. Carmen Montesinos
Summary: Activation of adenosine A(2A) receptor can promote angiogenesis by increasing the expression of annexin A2 in human dermal microvascular endothelial cells, which enhances fibrinolysis and contributes to wound healing and tissue revascularization.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Hematology
Jason S. Palazzolo, Anukreity Ale, Heidi Ho, Shweta Jagdale, Brad R. S. Broughton, Robert L. Medcalf, David K. Wright, Karen Alt, Christoph E. Hagemeyer, Be'eri Niego
Summary: A novel thrombolytic agent called SCE5-scuPA, which combines single-chain urokinase plasminogen activator (scuPA) with a single-chain antibody (SCE5) that targets the activated glycoprotein IIb/IIIa platelet receptor, showed improved clot degradation and potential for treating acute ischemic stroke in experimental studies.
Article
Health Care Sciences & Services
Hee Young Cho, Han Sung Park, Eun Hee Ahn, Eun Ju Ko, Hyeon Woo Park, Young Ran Kim, Ji Hyang Kim, Woo Sik Lee, Nam Keun Kim
Summary: The study revealed that PAI-1 rs1050955 and REN 10795 rs5707 alleles may decrease the risk of RPL, while the tPA rs4646972 genotype may increase the risk of RPL.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Obstetrics & Gynecology
Esra Demirel, Reem Sabouni, Neelima Chandra, Ov D. Slayden, David F. Archer
Summary: The study investigates the distribution and concentrations of several receptors and activators within the primate endometrium during artificial menstrual cycles, showing similarities in their expression patterns suggesting overlapping pathways. These findings indicate potential interactions between the plasminogen activator system and steroid receptors in the endometrium.
REPRODUCTIVE SCIENCES
(2022)
Article
Hematology
Julie Brogaard Larsen, Anne-Mette Hvas
Summary: This review highlights the key changes in fibrinolysis during sepsis, points out the current lack of reliable biomarkers for assessing fibrinolysis in clinical settings, and discusses the potential value of drugs targeting the fibrinolytic system in sepsis patients.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Engineering, Biomedical
Xingping Quan, Yan Han, Pengde Lu, Yuanfu Ding, Qingfu Wang, Yiyang Li, Jianwen Wei, Qiaoxian Huang, Ruibing Wang, Yonghua Zhao
Summary: This study developed a novel tPA delivery platform (APLT-PA) based on Annexin V and platelet membrane for the treatment of ischemic stroke. APLT-PA exhibited high targeting efficiency by binding to activated platelets and showed significant thrombolysis and improvement in neurological function in a mouse model.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Julie Brogaard Larsen, Mathies Appel Aggerbeck, Kim Michael Larsen, Christine Lodberg Hvas, Anne-Mette Hvas
Summary: This study found that septic shock patients exhibit distinct and abnormal clot-lysis profiles, which are associated with markers of coagulation and organ dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Yukiko Asano, Takayuki Iwaki, Kazuo Umemura, Naohiro Kanayama, Hiroaki Itoh
Summary: The study showed that fibrin had no effect on mouse blastocysts, but significantly suppressed trophoblast growth in human blastocysts. However, trophoblasts in human blastocysts then switched to growth promotion through increased fibrinolysis with urokinase-type plasminogen activator (uPA) activity.
HUMAN REPRODUCTION
(2021)
Article
Dentistry, Oral Surgery & Medicine
F. Pamuk, M. Lutfioglu, T. Paksoy, C. Z. Koyuncuoglu, N. G. Polat, E. Cifcibasi, S. Yildirim, A. Kantarci
Summary: The study found that low-level laser therapy (LLLT) as an adjunct to non-surgical periodontal treatment can modulate the plasminogen-activating system in severe periodontitis.
CLINICAL ORAL INVESTIGATIONS
(2023)
Review
Biochemistry & Molecular Biology
Hiroshi Kobayashi, Sho Matsubara, Chiharu Yoshimoto, Hiroshi Shigetomi, Shogo Imanaka
Summary: This review summarizes the biological functions of TFPI1 and TFPI2 in preeclamptic patients and discusses future directions in preeclampsia research. TFPI1 is an important physiological inhibitor of the TF-initiated extrinsic coagulation pathway, while TFPI2 inhibits fibrinolysis and exerts antifibrinolytic activity. TFPI1 and TFPI2 may play important roles in regulating the coagulation and fibrinolytic system and trophoblast invasion. Concentrations of TF, TFPI1, and TFPI2 are significantly altered in preeclamptic women compared to normal pregnancies. The TFPI protein family may serve as predictive biomarkers for preeclampsia and guide precision therapy.
Article
Immunology
Cecilia B. Korol, Serkan Belkaya, Fahad Alsohime, Lazaro Lorenzo, Stephanie Boisson-Dupuis, Joseph Brancale, Anna-Lena Neehus, Silvia Vilarinho, Alsum Zobaida, Rabih Halwani, Saleh Al-Muhsen, Jean-Laurent Casanova, Emmanuelle Jouanguy
Summary: Fulminant viral hepatitis (FVH) caused by hepatitis A virus (HAV) is a life-threatening disease. Inherited IL-10RB deficiency and possibly inherited IL-10 and IL-10RA deficiencies confer a predisposition to FVH. Vaccination is recommended for patients with these deficiencies.
JOURNAL OF CLINICAL IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Mohamed Haider, Roberta Cagliani, Jayalakshmi Jagal, Manju N. Jayakumar, Bahgat Fayed, Sarra B. Shakartalla, Renu Pasricha, Khaled Greish, Raafat El-Awady
Summary: This study aimed to develop a targeted nanotheranostic agent for early diagnosis and inhibition of malignant progression in colorectal cancer. Covalently labeled graphene oxide quantum dots showed increased targeting and specific uptake in colorectal cancer cells expressing the PLAC-1 antigen, leading to enhanced cell cytotoxicity and reduced invasiveness. This highlights the potential use of these targeted nanoparticles for detection and treatment of colorectal cancer.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alaa M. Altaie, Mohammad G. Mohammad, Mohamed I. Madkour, Sarra B. Shakartalla, Manju Nidagodu Jayakumar, K. G. Aghila Rani, Rabih Halwani, A. R. Samsudin, Rifat A. Hamoudi, Sameh S. M. Soliman
Summary: This study is the first to identify the pathological role of 17-ODYA in the development of periapical abscesses, providing important insights into the pathogenesis of periapical abscesses in relation to microbial metabolites.
JOURNAL OF ENDODONTICS
(2023)
Article
Biochemistry & Molecular Biology
Dali Vilma Francis, Manju Nidagodu Jayakumar, Hafiz Ahmad, Trupti Gokhale
Summary: The rising prevalence of antibiotic-resistance has led to the exploration and development of novel antimicrobial agents, such as biogenic CuO, ZnO, and WO3 nanoparticles, to address infections caused by drug-resistant pathogens. In this study, the antimicrobial effects of these nanoparticles were evaluated on clinical isolates of E. coli, S. aureus, MRSA, and Candida albicans. The results showed that photoactivated WO3 nanoparticles were the most promising antimicrobial agent, significantly reducing the number of viable cells for all test organisms. Additionally, combinations of CuO, ZnO, and WO3 nanoparticles demonstrated synergistic action, enhancing their antimicrobial properties compared to single elemental nanoparticles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Immunology
Narjes Saheb Sharif-Askari, Shirin Hafezi, Fatemeh Saheb Sharif-Askari, Naomi Frommenwiler, Rabih Halwani
JOURNAL OF CLINICAL IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Hezlin Marzook, Anamika Gupta, Dhanendra Tomar, Mohamed A. Saleh, Kiran Patil, Mohammad H. Semreen, Rifat Hamoudi, Nelson C. Soares, Rizwan Qaisar, Firdos Ahmad
Summary: Ischemia-induced metabolic remodeling is critical in the development of adverse cardiac remodeling and heart failure, however, its underlying molecular mechanism is largely unknown. This study identifies NRK-2 as a novel regulator of metabolic processes in the ischemic heart. Transcriptomic and metabolomic approaches reveal dysregulation of multiple cellular pathways and metabolites in NRK-2 knockout hearts post-myocardial infarction (MI), suggesting that NRK-2 is essential for metabolic adaptation in the ischemic heart.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Meeting Abstract
Physiology
Rakhee Ramakrishnan, Zaina Kalaji, Khuloud Bajbouj, Rabih Halwani, Qutayba Hamid
Article
Biology
Manju Nidagodu Jayakumar, Jibran Sualeh Muhammad, Mainak Dutta, Sainitin Donakonda
Summary: This study aims to identify and understand the involvement of chaperones in the pathogenesis of cholangiocarcinoma (CCA), a subtype of liver cancer. Through literature mining, expression analysis, and pathway analysis, the researchers identified novel therapeutic targets (CRYAB and P4HA2) and potential inhibitors (tucatinib and regorafenib) for CCA. The study provides new insights into the pan-chaperone expression in CCA and offers potential alternative approaches to its treatment.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Samrein B. M. Ahmed, Nada Radwan, Sara Amer, Narjes Saheb Sharif-Askari, Amena Mahdami, Kamel A. A. Samara, Rabih Halwani, Herbert F. F. Jelinek
Summary: Diabetes mellitus affects cellular functions through altered glucose metabolism and has been linked to cancer development. This study found that hyperglycemia combined with hyperinsulinemia has a potential effect on the progression of breast cancer cells, with differential expression of 45 genes observed. Additionally, 15 genes were found to be upregulated in breast tumor tissues of diabetic patients with breast cancer. These findings emphasize the importance of monitoring diabetes-related metabolic alterations in improving breast cancer outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Firdos Ahmad, Asima Karim, Javaidullah Khan, Rizwan Qaisar
Summary: This study evaluated the physical performance of patients with chronic heart failure (CHF) and found that those treated with statins had compromised handgrip strength, gait speed, and overall physical performance. The levels of plasma biomarkers, including CAF22, zonulin, and CRP, were significantly elevated in patients with CHF and associated with decreased physical performance. Furthermore, statin therapy in CHF patients was found to induce an increase in CAF22, zonulin, and CRP levels, leading to lower physical performance.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Firdos Ahmad, Hezlin Marzook, Anamika Gupta, Aseel Aref, Kiran Patil, Amir Ali Khan, Mohamed A. Saleh, Walter J. Koch, James R. Woodgett, Rizwan Qaisar
Summary: Reperfusion after acute myocardial infarction exacerbates cardiac injury and adverse remodeling. Loss of the GSK-3α isoform may attenuate cardiac injury and provide beneficial effects by limiting myocardial inflammation, mitochondrial dysfunction, and metabolic derangement. Selective inhibition of GSK-3α may be beneficial in ischemia/reperfusion-induced cardiac injury.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Multidisciplinary Sciences
Bushra Mdkhana, Narjes Saheb Sharif-Askari, Rakhee K. Ramakrishnan, Baraa Khalid Al-Sheakly, Shirin Hafezi, Fatemeh Saheb Sharif-Askari, Khuloud Bajbouj, Qutayba Hamid, Rabih Halwani
Summary: This study analyzed the role of the STING pathway in inducing pulmonary inflammation, steroid resistance, and remodeling in COPD. STING was found to be elevated in fibroblasts from healthy smokers and even more so in COPD patients compared to healthy non-smokers. Dexamethasone was effective in inhibiting STING activity in non-smoker fibroblasts but showed resistance in COPD fibroblasts. Treating COPD fibroblasts with a combination of STING inhibitor and dexamethasone alleviated remodeling and reversed steroid hyporesponsiveness.
Editorial Material
Immunology
Meganathan Kannan, Firdos Ahmad, Esaki M. Shankar
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Rizwan Qaisar, Asima Karim, M. Shahid Iqbal, Firdos Ahmad, Ahmad Shaikh, Hossam Kamli, Nizar A. Khamjan
Summary: This study investigated the association of elevated intestinal permeability with postural dysfunction in patients with Alzheimer's disease (AD). The results showed that elevated intestinal permeability is associated with postural dysfunction in AD patients and that plasma zonulin measurement can be used to diagnose postural dysfunction. This finding is important for non-ambulant and/or comatose AD patients and highlights the therapeutic potential of repairing the intestinal leak to improve postural control and reduce the risk of falls in AD patients.
Article
Biochemistry & Molecular Biology
Waad Kheder, Amal Bouzid, Thenmozhi Venkatachalam, Iman M. Talaat, Noha Mousaad Elemam, Tom Kalathil Raju, Soumya Sheela, Manju Nidagodu Jayakumar, Azzam A. Maghazachi, Abdul Rani Samsudin, Rifat Hamoudi
Summary: This study analyzed the gene expression in peri-implant gingival tissues of patients with failed, successful, or no implants. The results showed that titanium particles modulate lymphocyte and macrophage polarization in peri-implant tissues, which may contribute to implant failure. The presence of titanium particles and infiltration of immune cells, particularly lymphocytes and macrophages, were confirmed in the failed implant samples. In vitro validation also demonstrated increased expression of IL-1 beta, IL-8, and IL-18 by macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)