Dual growth factor entrapped nanoparticle enriched alginate wafer-based delivery system for suppurating wounds

We have investigated the wound healing efficiency of calcium alginate wafer embedded with growth factor entrapped PLGA nanoparticle. Herein, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) entrapped PLGA nanoparticles were synthesized and embedded in a sodium alginate gel by freezedrying technique. The synthesized dressing exhibited a high degree of swelling and appropriate porosity. The scaffold was characterized by Scanning Electron Microscopy (SEM) showing a highly porous morphology. Also, incorporation of growth factor loaded nanoparticles in a wafer-based delivery system resulted in localized growth factor delivery at the site of the wound in a sustained manner. The biocompatibility of the scaffold was evaluated by MTT assay, which showed a higher cell proliferation in the proposed scaffold as compared to the control. In vivo wound healing efficiency of the scaffold was evaluated using a full thickness murine wound model, which showed improved re-epithelialization, collagen deposition, and angiogenesis. These results suggest the use of the scaffold as a promising wound dressing material.

Automated summary

In this study, the wound healing efficiency of a calcium alginate wafer embedded with growth factor entrapped PLGA nanoparticles was investigated. The synthesized dressing exhibited a high degree of swelling and appropriate porosity, and the growth factor loaded nanoparticles in a wafer-based delivery system resulted in localized growth factor delivery at the wound site. The scaffold showed good biocompatibility and promoted re-epithelialization, collagen deposition, and angiogenesis in vivo. These findings suggest the potential use of this scaffold as a promising wound dressing material.