期刊
FEBS LETTERS
卷 596, 期 9, 页码 1214-1224出版社
WILEY
DOI: 10.1002/1873-3468.14337
关键词
3CL(pro); COVID-19; maturation; SARS-CoV-2; self-cleavage
资金
- Academia Sinica [AS-IDR-110-02]
- Ministry of Science and Technology, Taiwan [109-2745-B-001-001]
The self-cleavage rate of SARS-CoV-2 3CL(pro) is 35 times faster than that of SARS-CoV, implying that SARS-CoV-2 replication is more efficient.
The coronavirus (CoV) disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) has become a worldwide pandemic. The 3C-like protease (3CL(pro)), which cleaves 11 sites including its own N- and C-termini on the viral polyproteins, is essential for SARS-CoV-2 replication. In this study, we constructed the full-length inactive 3CL(pro) with N- and C-terminal extensions as substrates for monitoring self-cleavage by wild-type 3CL(pro). We found that the rate-limiting C-terminal self-cleavage rate of SARS-CoV-2 3CL(pro) was 35-fold faster than that of SARS-CoV 3CL(pro) using the Trx/GST-tagged C145A 3CL(pro) substrates. Since self-cleavage of 3CL(pro) is the initial step for maturation of other viral proteins, our study suggests more facile SARS-CoV-2 replication than that of SARS-CoV.
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