Autophagy promotes organelle clearance and organized cell separation of living root cap cells in Arabidopsis thaliana

The root cap is a multilayered tissue covering the tip of a plant root that directs root growth through its unique functions, such as gravity sensing and rhizosphere interaction. To maintain the structure and function of the root cap, its constituent cells are constantly turned over through balanced cell division and cell detachment in the inner and outer cell layers, respectively. Upon displacement toward the outermost layer, columella cells at the central root cap domain functionally transition from gravity-sensing cells to secretory cells, but the mechanisms underlying this drastic cell fate transition are largely unknown. Here, using live-cell tracking microscopy, we show that organelles in the outermost cell layer undergo dramatic rearrangements. This rearrangement depends, at least partially, on spatiotemporally regulated activation of autophagy. Notably, this root cap autophagy does not lead to immediate cell death, but is instead necessary for organized separation of living root cap cells, highlighting a previously undescribed role of developmentally regulated autophagy in plants.

Automated summary

The root cap is a tissue that guides root growth in plants. Its cells are constantly replaced through cell division and detachment. The transition of root cap cells from gravity-sensing cells to secretory cells involves rearrangement of organelles in the outer cell layer, which is partially regulated by autophagy. This developmentally regulated autophagy helps in the organized separation of living root cap cells, without causing immediate cell death.