Article
Biophysics
Attasith Parnsubsakul, Chaweewan Sapcharoenkun, Choochart Warin, Sanong Ekgasit, Prompong Pienpinijtham
Summary: This study investigated cryoprotectants for AuNPs that effectively prevent aggregation and identified sucrose, PVP, and pectin as suitable options. Additionally, the study emphasized the importance of washing restored AuNPs to ensure accurate results for subsequent applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Multidisciplinary Sciences
M. Huelsmeyer, D. Kuzman, M. Boncina, J. Martinez, C. Steinbrugger, J. Weusten, C. Calero-Rubio, W. Roche, B. Niederhaus, Y. VanHaelst, M. Hrynyk, P. Ballesta, H. Achard, S. Augusto, M. Guillois, C. Pszczolinski, M. Gerasimov, C. Neyra, D. Ponduri, S. Ramesh, D. Clenet
Summary: It is critical for biopharmaceutical companies to ensure the stability and activity of their fragile biomolecules during long-term storage and shipment. Advanced kinetic modeling (AKM) has been proven effective in predicting product shelf-life by utilizing data from short-term accelerated stability studies. The AKM methodology was evaluated for stability modeling of different types of biotherapeutics, vaccines, and biomolecules, and showed excellent agreement with experimental real-time data, confirming its reliability for stability predictions across various product types.
SCIENTIFIC REPORTS
(2023)
Article
Biophysics
Karim Chouchane, Thibaut Frachon, Laurent Marichal, Laurent Nault, Charlotte Vendrely, Antoine Maze, Franz Bruckert, Marianne Weidenhaupt
Summary: This study analyzes the effects of agitation on insulin aggregation and investigates the contribution of different agitation-induced effects on the rate of aggregation. The results show that strong agitation is necessary for the onset of insulin aggregation, and the presence of dynamic triple interfaces affects the rate of aggregation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Cathryn G. Conner, James McAndrew, Stefano Menegatti, Orlin D. Velev
Summary: This study reports a rapid method for evaluating the stability of antibody formulations using dynamic light scattering (DLS) to determine the size of particles in solution. The method involves analyzing high-resolution data, conducting temperature and stress tests, and plotting 3-D graphs to assess the stability of antibody solutions under different conditions.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Physical
Cathryn G. Conner, James McAndrew, Stefano Menegatti, Orlin D. Velev
Summary: A rapid method for determining the colloidal stability of protein molecules in solution has been reported, which serves as an efficient tool for evaluating the stability of antibody formulations. The method involves the use of human polyclonal immunoglobulin G (IgG) as a model protein and dynamic light scattering (DLS) to determine particle size in solution, enabling the investigation of aggregation rates at different temperatures and antibody concentrations.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Multidisciplinary
Wei Yao, Kaiyu Wang, Aide Wu, Wayne F. Reed, Bruce C. Gibb
Summary: The study reveals the complex interplay between non-covalent interactions of proteins and salts contributing to the Hofmeister effects, including interactions at different pH values and anion binding sites in ubiquitin. The results provide new insights into understanding the supramolecular properties of proteins.
Article
Chemistry, Physical
Visakh V. S. Pillai, Pallavi Kumari, Srikanth Kolagatla, Victoria Garcia Sakai, Svemir Rudic, Brian J. Rodriguez, Marina Rubini, Katarzyna M. Tych, Antonio Benedetto
Summary: This study reveals that ionic liquids can finely adjust the properties of amyloid fibrils, expanding the realm of basic science and applications. By investigating the effects of ethylammonium nitrate and tetramethyl-guanidinium acetate ionic liquids on lysozyme, it is found that they can modify the thickness, electric potential, and diameter of amyloid fibrils, and the mechanism of their action is elucidated.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Medicine, Research & Experimental
Lia M. Bersin, Sajal M. Patel, Elizabeth M. Topp
Summary: This study investigates the formation of pGlu from N-terminal glutamate in peptides and proteins, revealing differences in the pH dependence of the reaction rate between solid-state and solution reactions. Interestingly, the rate of pGlu formation in the solid state was found to be higher than in solution within the pH range commonly used for mAbs formulation. These findings have implications for stable formulations of peptides and proteins, as well as the transition from solid to solution formulations during development.
MOLECULAR PHARMACEUTICS
(2021)
Article
Chemistry, Physical
Samah Shanwar, Liuen Liang, Andrey V. Nechaev, Daria K. Bausheva, Irina V. Balalaeva, Vladimir A. Vodeneev, Indrajit Roy, Andrei V. Zvyagin, Evgenii L. Guryev
Summary: This study investigated the stabilizing effects of a denatured bovine serum albumin protein corona formed on the surface of upconversion nanoparticles, showing promise for improving photoluminescence brightness, sustained colloidal stability, and reduced level of serum protein surface adsorption.
Article
Chemistry, Medicinal
Ivonne Seifert, Alessandro Bregolin, Davide Fissore, Wolfgang Friess
Summary: A study was conducted to investigate the effects of different formulations of arginine with various counter ions on the stability of a monoclonal antibody. It was found that arginine hydrochloride provided the best protein stability at low antibody concentration, followed by arginine succinate, while arginine citrate/phosphate/lactobionate formulations showed inferior protein stability. Addition of sucrose improved cake appearance and protein stability in the formulations. In conclusion, sugar-free arginine hydrochloride and lactobionate lyophilisates demonstrated comparable or better stability than sucrose, with the best protein stability found in mixtures of arginine hydrochloride/lactobionate/succinate with sucrose.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Javed Masood Khan, Ajamaluddin Malik, Prerna Sharma, Sadaf Fatima
Summary: In this study, the impact of the negatively charged detergent sodium dodecyl sulfate (SDS) on insulin amyloid fibrillation was investigated. It was found that SDS can accelerate insulin aggregation at pH 2.0, but not at pH 7.4.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Olga Yu. Milyaeva, Alexander V. Akentiev, Alexey G. Bykov, Giuseppe Loglio, Reinhard Miller, Irina Portnaya, Anastasiya R. Rafikova, Boris A. Noskov
Summary: The dynamic surface properties of native kappa-casein solutions and their fibril dispersions are significantly different from those of globular proteins. The surface elasticity of kappa-casein solutions has a local maximum, indicating partial displacement of molecules from the proximal region to the distal region with increasing surface pressure. The surface elasticity of fibril dispersions shows a monotonic increase, similar to globular protein fibril dispersions. However, the surface elasticity of fibril dispersions can reach values approximately four times higher than that of native protein solutions at the same concentrations.
Article
Automation & Control Systems
Tai-Fang Li, Jiaming Lu, Jin Zhu
Summary: This paper proposes a dynamic model-based control strategy for improving the control accuracy of switched sampled-data control systems. By establishing a model at the controller side and controlling based on the model state, the exponential stability of the closed-loop system can be guaranteed.
JOURNAL OF THE FRANKLIN INSTITUTE-ENGINEERING AND APPLIED MATHEMATICS
(2022)
Review
Multidisciplinary Sciences
Nagavendra Kommineni, Arun Butreddy, Vaskuri G. S. Sainaga Jyothi, Pavimol Angsantikul
Summary: Freeze-drying serves as a robust solution to stability challenges in immunoengineering products, preserving the integrity and efficacy of active moieties. This review highlights the utility of lyophilization in maintaining the quality of various immunoengineering tools and products.
Article
Chemistry, Analytical
Anuj Shrivastava, Shyamapada Mandal, Sudip K. Pattanayek, Anurag S. Rathore
Summary: Aggregation of monoclonal antibody therapeutics is a serious concern. A new approach using dynamic light scattering (DLS) and machine learning (ML) algorithm is proposed to quantify the relative percentage of multimers in a monoclonal antibody therapeutic product.
ANALYTICAL CHEMISTRY
(2023)
Article
Spectroscopy
Charles H. Camp, Young Jong Lee, Marcus T. Cicerone
JOURNAL OF RAMAN SPECTROSCOPY
(2016)
Article
Chemistry, Physical
Marcus T. Cicerone, Madhusudan Tyagi
JOURNAL OF CHEMICAL PHYSICS
(2017)
Article
Chemistry, Physical
Mehmet Tatli, Hye Jin Chun, Charles H. Camp, Jingting Li, Marcus T. Cicerone, Wei-Chuan Shih, Jaan Laane, Timothy P. Devarenne
JOURNAL OF MOLECULAR STRUCTURE
(2017)
Article
Computer Science, Artificial Intelligence
Petru Manescu, Young Jong Lee, Charles Camp, Marcus Cicerone, Mary Brady, Peter Bajcsy
MEDICAL IMAGE ANALYSIS
(2017)
Editorial Material
Multidisciplinary Sciences
Charles H. Camp, Marcus T. Cicerone
Review
Chemistry, Analytical
Marcus T. Cicerone, Charles H. Camp
Article
Biochemistry & Molecular Biology
Wei-Wen Chen, George A. Lemieux, Charles H. Camp, Ta-Chau Chang, Kaveh Ashrafi, Marcus T. Cicerone
NATURE CHEMICAL BIOLOGY
(2020)
Article
Optics
Xavier Audier, Wei-Wen Chen, Marcus T. Cicerone
Article
Biochemical Research Methods
Daniel J. Lum, Michael D. Mazurek, Alexander Mikhaylov, Kristen M. Parzuchowski, Ryan N. Wilson, Ralph Jimenez, Thomas Gerrits, Martin J. Stevens, Marcus T. Cicerone, Charles H. Camp
Summary: This study demonstrates the preservation of time-energy entanglement of near-IR photons through thick biological media and tissue, supporting the use of nonclassical light in biological imaging and analyses. The entanglement characteristics of photons can be maintained even after propagation through thick, turbid biological samples.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biochemical Research Methods
Jessica Zahn, Arno Germond, Alice Y. Lundgren, Marcus T. Cicerone
Summary: This study compared the performance of two machine learning models in discriminating between Raman spectra of different bacterial strains of Escherichia coli. Both models were found to accurately distinguish between the strains, but with different classification boundaries. By analyzing the strain-specific spectral features used by the models, it was observed that they mainly utilized a small subset of high intensity peaks, with only one model utilizing lower intensity peaks.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Chemistry, Physical
John P. Stoppleman, Jesse G. McDaniel, Marcus T. Cicerone
Summary: This study shows the role of structural excitations in liquid relaxation and their relationship with density scaling. The study also discovers the equivalence between Gru''neisen parameter and density scaling parameter in interbasin crossing modes.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Physical
Rajas Poorna, Wei-Wen Chen, Peng Qiu, Marcus T. Cicerone
Summary: Raman spectroscopy has the ability to distinguish different cell phenotypes based on their metabolic profiles that change with transcriptomic activity. However, associating Raman spectral changes with specific signaling pathways can be challenging due to weak signals and individual variations. In this study, broadband coherent anti-Stokes Raman scattering (BCARS) microscopy is used to map the C. elegans hermaphrodite gonad at subcellular resolution. The results show that the BCARS spatio-spectral signatures correlate with gene expression profiles, indicating the potential of BCARS as a spatially resolved omics surrogate.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Correction
Chemistry, Physical
Rajas Poorna, Wei-Wen Chen, Arno Germond, Peng Qiu, Marcus T. Cicerone
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Physical
John S. Bender, Miaochan Zhi, Marcus T. Cicerone
Article
Biochemistry & Molecular Biology
Adrian B. Li, Jonathan A. Kluge, Miaochan Zhi, Marcus T. Cicerone, Fiorenzo G. Omenetto, David L. Kaplan