Retraction
Biotechnology & Applied Microbiology
W. Ruoming, Y. Zhen, Z. Tengteng, H. Jisheng
Summary: The article has been retracted. More details can be found in the Retraction Notice at https://doi.org/10.1038/s41417-021-00325-5.
CANCER GENE THERAPY
(2021)
Article
Oncology
Marta Correia de Sousa, Nicolas Calo, Cyril Sobolewski, Monika Gjorgjieva, Sophie Clement, Christine Maeder, Dobrochna Dolicka, Margot Fournier, Laurent Vinet, Xavier Montet, Jean-Francois Dufour, Bostjan Humar, Francesco Negro, Christine Sempoux, Michelangelo Foti
Summary: Despite being considered a potent oncomiR in human cancers, genetic deletion of miR-21 in mouse models surprisingly promotes hepatocellular carcinoma (HCC) development by affecting various signaling pathways and immune responses. These results challenge the current belief in miR-21 as an oncogene and raise caution regarding using miR-21 inhibition as a therapeutic approach for HCC.
Article
Chemistry, Analytical
Yong Wang, Dandan Wang, Guohua Qi, Ping Hu, Erkang Wang, Yongdong Jin
Summary: In this study, a gold nanoparticles nanomembrane-modified plasmonic glass nanopipette surface-enhanced Raman scattering (SERS) sensing platform was constructed to sensitively detect the content variation of intracellular miRNA-21. The proposed method provides a simple way to study intracellular substance change and evaluate cancer treatment outcomes.
ANALYTICAL CHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Shigeo Hisamori, Junko Mukohyama, Sanjay Koul, Takanori Hayashi, Michael Evan Rothenberg, Masao Maeda, Taichi Isobe, Luis Enrique Valencia Salazar, Xin Qian, Darius Michael Johnston, Dalong Qian, Kaiqin Lao, Naoya Asai, Yoshihiro Kakeji, Vincenzo Alessandro Gennarino, Debashis Sahoo, Piero Dalerba, Yohei Shimono
Summary: In this study, it was found that the coordinated upregulation of miR-200c and miR-203 in colon epithelial cells is associated with terminal differentiation and plays a role in controlling the expansion capacity and stem cell properties of epithelial cells by suppressing BMI1 expression.
JOURNAL OF GASTROENTEROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Petra Korac, Mariastefania Antica, Maja Matulic
Summary: miR-7 is an ancient miRNA involved in cell signaling pathways, acting primarily as a tumor suppressor by inhibiting cell proliferation and promoting apoptosis. It also has additional roles in cell regulation and protection, with potential for use in biotherapeutics.
Article
Biotechnology & Applied Microbiology
Daoyuan Wang, Shuxian Yan, Lihui Wang, Yunlong Li, Baoping Qiao
Summary: This study demonstrated that circSLC8A1 was downregulated in prostate cancer, leading to the inhibition of cell proliferation and migration. CircSLC8A1 interacted directly with miR-21 and acted as a miRNA sponge to inhibit PCa progression, potentially regulating cell proliferation, angiogenesis, cell migration, and other pathways.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Guoqiao Huang, Hongyin Zhou, Qi Xiang, Jing Zhang, Xuemei Hu, Ruize Cheng, Linwen Lan, Yuzhe Wang, Zhifa Shen
Summary: In this study, a well-designed detection platform based on rolling circle amplification (RCA) was developed, enabling the quantitative detection of miRNAs and p53 gene as well as potential application in clinical diagnosis and biomedical research.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Divya Reddy, Saikat Bhattacharya, Sanket Shah, Mudasir Rashid, Sanjay Gupta
Summary: The study demonstrates the downregulation of histone H3 variant H3.3 and the upregulation of H3.2 in HCC, leading to alterations in histone modifications associated with active transcription. Specific histone chaperones CAF-1 and HIRA may play a role in regulating H3.3 and H3.2 levels. Inhibition of DNA methylation and histone deacetylation could potentially restore histone H3 variant expression in tumor cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Surendra Kumar Sagar
Summary: This review focuses on the function of miR-106b and its downstream targets in cancer, providing insights into how miR-106b regulates cancer cell proliferation, migration, invasion, and metastasis by modulating tumor suppressor genes. The potential of miR-106b as a candidate for detection, diagnosis, and prognosis assessment in different cancer types is highlighted.
Article
Oncology
Ai Zhao, Fanfan Li, Cheng Wei, Zhujun Zhou, Xianqiang Luo, Haiming Wu, Chunhong Ning, Wanyu Liu, Dong Li, Danni Lin, Shuwen Liu, Guangji Zhang, Jimin Gao
Summary: The study reveals the role of myeloid-derived suppressor cells (MDSCs) in the development of B[a]P-induced chronic lung inflammation and the efficacy of immunotherapy in preventing malignant transformation. TNFalpha antagonist alone or with PD-1 blocker can prevent or retard the malignant transformation of B[a]P-induced chronic lung inflammation, especially in the early stage.
Article
Immunology
Qi Zhang, Jing Pan, Donghai Xiong, Junjun Zheng, Kristi N. McPherson, Sangbeom Lee, Mofei Huang, Yitian Xu, Shu-hsia Chen, Yian Wang, Lea Hildebrandt Ruiz, Ming You
Summary: Delivery of aerosolized microRNAs targeting PD-L1 can effectively prevent the development and progression of lung cancer. In this study, aerosolized miR-138-5p and miR-200c miRNA mimics were found to inhibit B(a)P-induced lung adenomas and NTCU-induced lung squamous cell carcinomas with no detectable side effects. These miRNAs were also shown to inhibit PD-L1 expression and promote the immune response against cancer cells by increasing CD4+ and CD8+ T cells while reducing PD-1 expression and T-regulatory cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Janson Tse, Thomas Pierce, Annalisa L. E. Carli, Mariah G. Alorro, Stefan Thiem, Eric G. Marcusson, Matthias Ernst, Michael Buchert
Summary: MicroRNA-21 (miR-21) is a small, non-coding RNA that is overexpressed in gastric cancer and other solid malignancies, with both pro- and anti-tumorigenic properties. However, the regulation of miR-21 and the effects of its inhibition in gastric cancer are not fully understood. In this study, the researchers investigated the molecular pathways that control miR-21 expression in gastric cancer and evaluated the therapeutic potential of targeting miR-21 with a synthetic miR-21 antagomir. They found that miR-21 expression in early gastric cancer is dependent on Stat3, which is activated by IL-6 family cytokines through the gp130 receptor. Inhibition of miR-21 with the antagomir reduced gastric tumor growth, epithelial-to-mesenchymal transition, and matrix remodeling. The study highlights miR-21 as a promising target for anti-cancer therapy in gastric cancer.
Review
Medicine, Research & Experimental
Seyede Fatemeh Hosseini, Setareh Javanshir-giv, Hanieh Soleimani, Homa Mollaei, Farzad Sadri, Zohreh Rezaei
Summary: MicroRNA production in tumorigenesis is dysregulated by various processes, leading to dysfunctional and dysregulated miRNAs. These miRNAs have been identified as potential biomarkers for human cancer, although further evaluation is necessary. The miR-28 family, especially miR-28-5p and miR-28-3p, play essential roles in various cancers and can serve as diagnostic biomarkers for prognosis and early detection of cancers.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Holly Holliday, Jessica Yang, Eoin Dodson, Iva Nikolic, Alvin Kamili, Madeleine Wheatley, Niantao Deng, Sarah Alexandrou, Thomas P. Davis, Maria Kavallaris, C. Elizabeth Caldon, Joshua McCarroll, Katleen De Preter, Pieter Mestdagh, Glenn M. Marshall, Kaylene J. Simpson, Jamie Fletcher, Alexander Swarbrick
Summary: This study discovered that miR-99b-5p, miR-380-3p, and miR-485-3p enhance the chemotherapeutic effects of doxorubicin in high-risk neuroblastoma. These miRNAs are often lost in patients and their low expression predicts poor survival outcomes.
Review
Cell Biology
Milad Rafat, Mahta Moraghebi, Masoumeh Afsa, Kianoosh Malekzadeh
Summary: MiR-132 plays important roles in various cancers as a potential biomarker and tumor suppressor, involved in regulating cell cycle pathways and oncogenes. It could potentially be a candidate for early detection and prognosis assessment in cancer.
Article
Biochemistry & Molecular Biology
Esmaeil Darvish Aminabad, Mohammad Hasanzadeh, Ali Ahmadalipour, Tohid Mahmoudi, Mohammad Ali Hosseinpour Feizi, Reza Safaralizadeh, Ahmad Mobed
Summary: This research work developed an electrochemical immunosensor for the selective recognition of SNCA in human biofluids. It proposed an innovative protocol for the green synthesis of AuNPs@DMGO and designed a sandwich immunosensor for the sensitive identification of SNCA antigen. The method showed a potential of employment in clinical applications and high-throughput screening of Parkinson's disease using POC.
JOURNAL OF MOLECULAR RECOGNITION
(2023)
Review
Pharmacology & Pharmacy
Jafar Sadeghzadeh, Parviz Shahabi, Mehdi Farhoudi, Abbas Ebrahimi-Kalan, Ahmad Mobed, Kourosh Shahpasand
Summary: Tau protein is crucial in diagnosing neurodegenerative diseases, but detecting it on a nanoscale is challenging. Methods like ELISA, western-blotting, and molecular-based techniques have limitations. Biosensors are a promising approach to overcome these limitations and monitor tau protein.
ADVANCED PHARMACEUTICAL BULLETIN
(2023)
Review
Microbiology
Naser Alizadeh, Ghazal Naderi, Mohammad Saeed Kahrizi, Tannaz Haghgouei, Ahmad Mobed, Mehran Ebrahimi Shah-abadi
Summary: The relationship between gut microbiota and pain has gained attention, with evidence suggesting that microbiota is an important pain modulator in the central and peripheral nervous systems. Changes in microbiota through diet or antibiotic mediation may offer a novel therapeutic strategy for pain management.
CURRENT MICROBIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Mohammad Hossein Ayati, Mostafa Araj-Khodaei, Tannaz Haghgouei, Ali Ahmadalipour, Ahmad Mobed, Sarvin Sanaie
Summary: The gut microbiota plays an important role in clinical oncology, affecting not only the risks and causes of cancer, but also the immune response of intestinal and extra-intestinal tumors. Nanomaterial-based biosensors show potential in monitoring and detecting probiotics for quality and safety management. However, point-of-care biosensors specifically for detecting microbial community disruptions in the human gut flora are not yet available in clinical practice.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Biochemical Research Methods
Ahmad Mobed, Fereshteh Kohansal, Sanam Dolati, Mohammad Hasanzadeh
Summary: The aim of this study was to develop a label-free electrochemical biosensor for detecting telomerase in human biofluid. A green nanocomposite (poly[chitosan] decorated by gold nanoparticles) was used to immobilize the biotinylated antibody of telomerase and the immunocomplex of antigen-antibody. The constructed immunosensor showed a wide dynamic range (0.078-160 IU/ml(-1)) with a low limit of quantification of 0.078 IU/ml(-1), providing a unique approach for telomerase assays in early cancer prognosis.
Review
Engineering, Biomedical
Hamidreza Hassanzadeh Khanmiri, Fatemeh Yazdanfar, Ahmad Mobed, Fatemeh Rezamohammadi, Mehrnoush Rahmani, Tannaz Haghgouei
Summary: Early diagnosis of CRP is crucial for effective treatment of related diseases. Current diagnostic techniques, mostly used in central laboratories, are expensive, time-consuming, and require specialized personnel. Point-of-care (POC) biosensors have emerged as a promising alternative, allowing regular health monitoring without laboratory tests.
BIOMEDICAL MICRODEVICES
(2023)
Review
Cell Biology
Ahmad Mobed, Saeid Charsouei, Yalda Yazdani, Morad Kohandel Gargari, Ali Ahmadalipour, Seyyedeh Reyhaneh Sadremousavi, Maryam Farrahizadeh, Ali Shahbazi, Maryam Haghani
Summary: Key biomarkers like BDNF and NfL are crucial for the development and progression of neurological diseases. The discovery of robust biomarkers is important for early detection and monitoring of these diseases. Recent advances in biotechnology and nanotechnology have allowed for real-time detection of multiple biomarkers, making screening programs and personalized medicine possible.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Review
Cell Biology
Jafar Sadeghzadeh, Leila Hosseini, Ahmad Mobed, Hamid Soltani Zangbar, Jaber Jafarzadeh, Jamshid Pasban, Parviz Shahabi
Summary: Cerebral ischemia and subsequent reperfusion can lead to reduced blood supply to specific brain areas, resulting in neurological damage and irreversible harm. The hippocampus is particularly vulnerable to ischemia-induced injuries, with the extent of damage influenced by the different stages of ischemia. Intricate biochemical reactions involving free radicals, oxidative stress, inflammatory responses, and glutamate toxicity contribute to various changes and damage in neural tissue. Antioxidant enzymes play a crucial role in preventing hippocampal damage caused by oxidative stress.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Analytical
Fereshteh Kohansal, Ahmad Mobed, Nastaran Aletaha, Kambiz Ghaseminasab, Sanam Dolati, Mohammad Hasanzadeh
Summary: A novel sandwich-type electrochemical immunosensor was developed for efficient detection of telomerase antigen. The sensor exhibited excellent selectivity and stability for telomerase detection in human plasma samples, showing great potential for clinical diagnosis of cancer.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Ahmad Mobed, Fereshteh Kohansal, Sanam Dolati, Mohammad Hasanzadeh, Seyed Kazem Shakouri
Summary: Hyaluronic acid (HA) is a glycosaminoglycan that is synthesized by connective tissue cells and plays a critical role in tissue fibrosis and tumor progression. This study developed an immune device for the sensitive detection of HA in human plasma samples using an innovative alloy substrate. The developed immunosensor has a linear response within a specific concentration range and offers a promising technique for the early diagnosis of disorders related to abnormal levels of HA.
Review
Multidisciplinary Sciences
Ahmad Mobed, Sarah Gholami, Amir Tahavvori, Farhood Ghazi, Zahra Masoumi, Iraj Alipourfard, Ramtin Naderian, Mehran Mohammadzadeh
Summary: This study reviews the latest biosensors developed for the detection of antihypertensive drugs (AHTDs). Biosensors have been widely used for detecting biomarkers and drugs due to their inherent advantages. Through this study, readers will gain a deeper understanding of biosensors as advanced diagnostic tools.
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Materials Science, Biomaterials
Maryam Hariri, Vahid Alivirdiloo, Nastaran Safavi Ardabili, Sarah Gholami, Sajjad Masoumi, Mohammad Reza Mehraban, Mahsa Alem, Reza Shah Hosseini, Ahmad Mobed, Farhood Ghazi, Iraj Alipourfard
Summary: The precise detection of cancer biomarkers is crucial for effective diagnosis, monitoring, and treatment. CEA, a protein found in minimal amounts in adult blood, can be elevated in certain diseases and types of cancer. It is important to develop a sensitive and simple CEA detection method to accurately diagnose cancer and improve patient survival. Biosensors show promise as a new direction in medical oncology, offering rapid response, high sensitivity, and convenience in early disease detection.
Article
Chemistry, Multidisciplinary
Ahmad Mobed, Fereshteh Kohansal, Sanam Dolati, Mohammad Hasanzadeh
Summary: LYVE-1 is a hyaluronan receptor expressed on lymphatic vessel endothelium, involved in tumor cell adhesion and lymphocyte passage through the lymphatics. A paper-based electrochemical immune-platform was developed for the recognition of LYVE-1, featuring small size, low cost, high stability, and sensitivity. This biosensor has potential applications in tumor lymphangiogenesis research and biomarker-related disease screening in clinical studies.
Article
Chemistry, Multidisciplinary
Fereshteh Kohansal, Ahmad Mobed, Rana Ansari, Mohammad Hasanzadeh, Ali Ahmadalipour, Nasrin Shadjou
Summary: The endocannabinoid system is crucial for regulating cognitive and physiological processes, with 2-AG being one of the key endocannabinoids. Rapid and specific diagnosis of 2-AG is important in medical neuroscience and has been a focus of recent research. In this study, a novel immunosensor was developed for quantifying 2-AG, demonstrating high sensitivity and specificity.
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)