4.6 Article

Antiproliferative potentials of chitin and chitosan encapsulated gold nanoparticles derived from unhatched Artemia cysts

期刊

CHEMICAL PHYSICS LETTERS
卷 790, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.cplett.2022.139345

关键词

Artemia cysts; Chitin and chitosan; Gold nanoparticles; Anticancer activity; A549 cells; HT-29 cells

资金

  1. Department of Science & Technology (DST) -PURSE
  2. King Saud University, Riyadh, Saudi Arabia [RSP-2021/355]

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This study reports the synthesis, characterization, and testing of photon induced gold nanoparticles (AuNPs) encapsulated in chitin and chitosan derived from unhatched Artemia cysts. The chitosanAuNPs exhibited a potential cytotoxic effect against human lung cancer cells (A549) compared to chitin-AuNPs. The findings suggest that these biopolymers have the potential to be used as anticancer agents with specific applications in biomedical imaging and drug delivery.
The recent increase in the usage of metallic nanoparticles (NPs) individually or the one coated with polymeric shells in the biomedical sector are helpful to control the unwanted toxicities, non-targeted localizations, enhance the probe-cellular interactions etc. Herein, we report the synthesis, characterization, and testing of photon induced gold (Au) NPs encapsulated chitin and chitosan, which are derived from unhatched Artemia cysts. In this, the chitin and chitosan biopolymers are formed by a biosynthetic route and then subjected to a reaction in the presence of HAuCl4 for the encapsulation of AuNPs. Thus formed NPs are thoroughly studied for the surface morphology, functionality, and crystallinity using SEM/TEM, DLS, and FTIR respectively. Further, the anti proliferative capacity of as-synthesized chitin and chitosan stabilized AuNPs are being tested using two different cell types of human colon cancer (HT-29) and lung cancer (A549). The human tumor cell lines confirm the specific cytotoxicity. The optimized concentration of chitin and chitosan-loaded AuNPs showed a maximum anticancer activity in human lung cancer cells (A549) as compared with colon cancer (HT-29). Also, the chitosanAuNPs have a potential cytotoxic effect against the human lung cancer cell line (A549) than that of the corresponding chitin-AuNPs. Finally, the study utilizes chitin and chitosan biopolymers generated from uncharted Artemia cysts for the formation of useful anticancer agents that has specific applications in the biomedical sector as bioimaging and drug delivering candidates.

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