Article
Gastroenterology & Hepatology
Zheng Zhou, Wenjing Yang, Tianming Yu, Yu Yu, Xiaojing Zhao, Yanbo Yu, Chuncai Gu, Anthony J. Bilotta, Suxia Yao, Qihong Zhao, George Golovko, Mingsong Li, Yingzi Cong
Summary: This study investigated the role of GPR120 in regulating neutrophil function against enteric bacteria. Gpr120 (-/-) mice showed altered gut microbiota composition and exhibited more severe intestinal inflammation upon infection or DSS insults. GPR120 agonist enhanced neutrophil production of reactive oxygen species and extracellular traps, leading to inhibition of bacterial growth.
Review
Biochemistry & Molecular Biology
Michael J. Wright, Guangchun Bai
Summary: This review summarizes the c-di-AMP networks in seven species of the genus Streptococcus, which cause diverse clinical manifestations, and highlights their important roles in biological outcomes and interaction with hosts.
MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jana L. Heidemann, Piotr Neumann, Larissa Krueger, Dennis Wicke, Liza Vinhoven, Andreas Linden, Achim Dickmanns, Joerg Stuelke, Henning Urlaub, Ralf Ficner
Summary: The bacterial second messenger c-di-AMP controls essential cellular processes, and its binding to the protein DarB regulates the stringent response. We determined the crystal structures of DarB in different states and found that DarB forms a homodimer, binding two cyclic dinucleotide molecules or two AMP molecules. Our findings provide insights into the mechanism of stringent response regulation by c-di-AMP and have implications for drug development.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yu Jiang, Xiyuan Li, Fenghui Qian, Bingbing Sun, Xiyuan Wang, Yan Zhang, Deqiang Zhang, Meiyu Geng, Zuoquan Xie, Sheng Yang
Summary: This study optimized the translation levels of diadenylate cyclase for the production of cyclic di-adenosine monophosphate (CDA) in two engineered bacterial strains. Although one strain showed stronger induction of the STING pathway, it had lower antitumor activity in an allograft tumor model, possibly due to the stability of the bacteria in the tumor tissue. The other strain exhibited complete tumor regression, prolonged survival of mice, and rejection of rechallenged tumors, suggesting new possibilities for effective tumor therapy.
Article
Microbiology
Zhuo Wang, Li Song, Xiaozhen Liu, Xihui Shen, Xin Li
Summary: In natural environments, bacteria rely on the nucleotide signaling molecule c-di-GMP to regulate their behaviors and adapt to stressful conditions. c-di-GMP acts as a second messenger that triggers a stress response in bacterial cells. The production of extracellular matrix in biofilms provides a physical barrier for bacterial cells, protecting them from environmental assaults. This review summarizes recent research on the role of c-di-GMP signaling in bacterial stress responses.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Xiaofei Gao, Yang Song, Jiali Wu, Shuang Lu, Xiaoli Min, Limin Liu, Longyuan Hu, Meiling Zheng, Pei Du, Yaqin Yu, Hai Long, Haijing Wu, Sujie Jia, Di Yu, Qianjin Lu, Ming Zhao
Summary: Iron plays an important role in regulating pathogenic T cell differentiation and autoantibody production in SLE. High-iron diet promotes Tfh cell expansion, and the miR-21/BDH2 axis may be critical for iron accumulation during Tfh cell differentiation.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Tatiana B. Cereija, Joao P. L. Guerra, Joao M. P. Jorge, Joao H. Morais-Cabral
Summary: bis-(3',5')-cyclic diadenosine monophosphate (c-di-AMP) is a second messenger that plays various roles in bacteria, including regulation of virulence, cell wall formation, biofilm formation, and DNA integrity surveillance. It has been shown to have a regulatory effect on the activity of K+ homeostasis machinery, inhibiting K+ import and activating K+ export. Experimental data suggest that c-di-AMP activates KhtTU through a specific mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Karen Abundiz-Yanez, Hilda C. Leyva-Sanchez, Eduardo A. Robleto, Mario Pedraza-Reyes
Summary: A study investigated the role of c-di-AMP in stress-associated mutagenesis (SAM) and growth-associated mutagenesis (GAM) in B. subtilis. The results showed that DACs, CdaA and DisA, played important roles in cell wall homeostasis and chromosomal fidelity, and counteracted mutagenesis in growing cells. However, deficiencies in DACs caused opposite effects under oxidative stress. DACs also promoted mutations under nutritional stress in the stationary phase. The study suggests that c-di-AMP is involved in promoting genetic diversity in growth-limiting conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Beata Tokarz-Deptula, Joanna Palma, Lukasz Baraniecki, Michal Stosik, Roman Kolacz, Wieslaw Deptula
Summary: Platelets play a role in inflammation and infections by reacting with the endovascular environment and cells of the immune system. This role is influenced by biologically active substances present in platelet granules and EV structures.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Rida Al-Rifai, Marie Vandestienne, Jean-Remi Lavillegrand, Tristan Mirault, Julie Cornebise, Johanne Poisson, Ludivine Laurans, Bruno Esposito, Chloe James, Olivier Mansier, Pierre Hirsch, Fabrizia Favale, Rayan Braik, Camille Knosp, Jose Vilar, Giuseppe Rizzo, Alma Zernecke, Antoine-Emmanuel Saliba, Alain Tedgui, Maxime Lacroix, Lionel Arrive, Ziad Mallat, Soraya Taleb, Marc Diedisheim, Clement Cochain, Pierre-Emmanuel Rautou, Hafid Ait-Oufella
Summary: JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Hua Cai, Lihua Li, Kailey M. Slavik, Jingxian Huang, Ting Yin, Xianlong Ai, Lena Hedelin, Gabrielle Haas, Zhangmin Xiang, Yunyun Yang, Xiaoyan Li, Yuqiang Chen, Ziming Wei, Huimin Deng, Di Chen, Renjie Jiao, Nelson Martins, Carine Meignin, Philip J. Kranzusch, Jean-Luc Imler
Summary: The study revealed the presence of two cGAS-like receptors in Drosophila melanogaster, which produce cyclic dinucleotides (CDNs) that play a role in immune response. The researchers found that 2'3'-cGAMP and 3'2'-cGAMP were ineffective in controlling the infection by Drosophila C virus. However, they identified a more potent STING agonist called 2'3'-c-di-GMP, which activated a strong antiviral transcriptional response in Drosophila serrata. These findings provide insights into the evolution and function of cGAS-like receptors in flies and their role in innate immunity.
Article
Chemistry, Multidisciplinary
Yi Wang, Yannan Zhao, Jiangling Wu, Ming Li, Juan Tan, Wensheng Fu, Hua Tang, Pu Zhang
Summary: The study engineered negatively charged sulfur quantum dots (SQDs) as an efficient broad-spectrum antibiotic to kill drug-resistant bacteria in vitro and in vivo. SQDs destroy bacterial membrane systems and affect their metabolism through the intrinsic antibacterial activity of elemental sulfur and catalytic generation of reactive oxygen species. Compared to traditional positively charged materials, SQDs demonstrate better hemocompatibility and lower toxicity.
Review
Immunology
Gael Galli, Maya Saleh
Summary: Macrophages play crucial roles in tissue homeostasis, inflammation, and host defense by recognizing microbial or danger signals and eliciting immune responses. The interaction between cellular metabolism and macrophage innate immunity involves metabolic adaptations that modulate immune signaling and macrophage function. Intracellular bacterial pathogens can exploit macrophage metabolic pathways to evade immune defenses. This review highlights recent evidence on host-bacterial immunometabolic interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Yanzhi Lu, Huanhuan Ning, Jian Kang, Guangchun Bai, Lei Zhou, Yali Kang, Zhengfeng Wu, Maolin Tian, Junhao Zhao, Yueyun Ma, Yinlan Bai
Summary: Many antigens from Mycobacterium tuberculosis have strong immunogenicity and potential as vaccine candidate antigens. In this study, the researchers explored the immunogenicity and immune responses induced by the bacterial second messenger Cyclic di-AMP (c-di-AMP) phosphodiesterase (CnpB) in mice. They found that CnpB had strong immunogenicity and induced high levels of humoral response and lung mucosal immunity after M. tuberculosis infection. CnpB also interfered with host innate and adaptive immune responses and conferred protection against M. tuberculosis respiratory infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Yi Gong, Jiaxi Li, Lili Huang, Yi Liu, Ju Cao
Summary: This study found that CXCL16 levels were significantly higher in the serum of sepsis patients compared to healthy volunteers, and correlated with disease severity. Soluble CXCL16 levels had significant prognostic value for 28-day mortality in sepsis patients. The study also showed that CXCL16 could be a potential target for therapeutic intervention in sepsis treatment.
MICROBES AND INFECTION
(2022)
