期刊
CARDIOVASCULAR RESEARCH
卷 118, 期 17, 页码 3331-3345出版社
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvac052
关键词
myocarditis; Inflammation; Neutrophils; Neutrophil extracellular traps; Coxsackievirus B3
资金
- European Research Area Network on Cardiovascular Diseases (ERA-Net-CVD) MacroERA [01KL1706]
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) [Vidi 91796338]
- Fonds Wetenschappelijk Onderzoek (FWO) [G091018N, G0B5920N, 1514518N]
The study investigates the contribution of neutrophils to the progression of viral myocarditis (VM) and found that inhibiting neutrophils and neutrophil extracellular traps (NETs) in the acute phase of VM can reduce cardiac inflammation and improve the pathological phenotype.
Aims Viral myocarditis (VM) is an inflammatory pathology of the myocardium triggered by a viral infection that may cause sudden death or heart failure (HF), especially in the younger population. Current treatments only stabilize and improve cardiac function without resolving the underlying inflammatory cause. The factors that induce VM to progress to HF are still uncertain, but neutrophils have been increasingly associated with the negative evolution of cardiac pathologies. The present study investigates the contribution of neutrophils to VM disease progression in different ways. Methods and results In a coxsackievirus B3-(CVB3) induced mouse model of VM, neutrophils and neutrophil extracellular traps (NETs) were prominent in the acute phase of VM as revealed by enzyme-linked immunosorbent assay analysis and immunostaining. Anti-Ly6G-mediated neutrophil blockade starting at model induction decreased cardiac necrosis and leucocyte infiltration, preventing monocyte and Ly6CHigh pro-inflammatory macrophage recruitment. Furthermore, genetic peptidylarginine deiminase 4-dependent NET blockade reduced cardiac damage and leucocyte recruitment, significantly decreasing cardiac monocyte and macrophage presence. Depleting neutrophils with anti-Ly6G antibodies at 7 days post-infection, after the acute phase, did not decrease cardiac inflammation. Conclusion Collectively, these results indicate that the repression of neutrophils and the related NET response in the acute phase of VM improves the pathological phenotype by reducing cardiac inflammation. [GRAPHICS] .
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