Article
Pharmacology & Pharmacy
Wu Luo, Gaojun Wu, Xiaojun Chen, Qiuyan Zhang, Chunpeng Zou, Jun Wang, Jun Liu, Nipon Chattipakorn, Yi Wang, Guang Liang
Summary: The expression of MyD88 protein was found to be increased in the hearts of diabetic mice. Inhibition of MyD88 through siRNA or small-molecular inhibitor markedly reduced inflammatory response and associated cell signaling pathway activation under high glucose conditions. Furthermore, pharmacological inhibition of MyD88 showed anti-inflammatory, anti-hypertrophic, and anti-fibrotic effects in diabetic mouse hearts. These findings indicate that MyD88 may serve as a potential therapeutic target for diabetic cardiomyopathy.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Hui Lin, Le Guan, Liping Meng, Hiroyasu Uzui, Hangyuan Guo
Summary: SGLT1 is upregulated in diabetic cardiac tissues and high-glucose-induced cardiac fibroblasts. Its overexpression promotes cardiac fibroblast proliferation and collagen synthesis, while its silencing significantly alleviates cardiac fibrosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Physiology
Jiajia Yang, Mariana A. Argenziano, Mariana Burgos Angulo, Alexander Bertalovitz, Maliheh Najari Beidokhti, Thomas V. McDonald
Summary: The study generated patient-specific induced pluripotent stem cell lines with various LMNA mutations, differentiating them into cardiomyocytes and cardiac fibroblasts to investigate the phenotypic variability and mechanistic differences caused by these mutations. Results showed diverse abnormalities in LMNA expression, cell morphology, and electrophysiological properties, indicating the utility of this experimental platform in exploring the heterogeneity of mutations within cardiac disease-associated genes.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Bingying Dai, Liqing Ding, Lijuan Zhao, Honglin Zhu, Hui Luo
Summary: This review summarizes the current knowledge on immune cells and stromal cells in SSc patients and discusses their potential roles in the pathogenesis of SSc, with a focus on recent advances in new subtypes by scRNA-seq.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Sadek Munawar, Irene C. Turnbull
Summary: Nonmyocytes play a crucial role in enhancing the features of engineered cardiac tissues (ECTs), with co-culture aiming to mimic the cellular composition of the native heart for better tissue function and maturity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Jian-Kui Du, Qing Yu, Yu-Jian Liu, Shu-Fang Du, Li-Yang Huang, Dan-Hong Xu, Xin Ni, Xiao-Yan Zhu
Summary: The study reveals the role of KLK8 in diabetic cardiomyopathy, with KLK8 deficiency attenuating cardiac fibrosis while KLK8 overexpression inducing EndMT. This provides a new direction for therapeutic strategies for diabetic cardiomyopathy.
Article
Cardiac & Cardiovascular Systems
Xide Shi, Chao Liu, Jiangwei Chen, Shiqiang Zhou, Yajuan Li, Xingcheng Zhao, Jinliang Xing, Junhui Xue, Fengzhou Liu, Fei Li
Summary: In this study, it was found that MICU1 expression is decreased in myocardial microvascular endothelial cells (CMECs) of diabetic mice. Overexpression of MICU1 can alleviate cardiac hypertrophy and fibrosis, and improve cardiac function in diabetic mice. These findings suggest that MICU1 may be a potential intervention target for the treatment of diabetic cardiomyopathy (DCM).
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Endocrinology & Metabolism
Fangfang Zeng, Peng Zhou, Meng Wang, Lijie Xie, Xinmei Huang, Yilin Wang, Jinya Huang, Xiaoqing Shao, Yeping Yang, Wenjuan Liu, Maocheng Gu, Yifei Yu, Fei Sun, Min He, Yiming Li, Zhaoyun Zhang, Wei Gong, Yi Wang
Summary: The study found that the NAD+ level is lower in the heart of diabetic cardiomyopathy. Supplementation with nicotinamide can increase NAD+ level and improve myocardial capillary density and fibrosis. The study also found that the expression of ACMSD is increased in myocardial endothelial cells of diabetic mice, and high glucose can promote the expression of ACMSD. Inhibition of ACMSD can increase NAD+ synthesis and improve endothelial cell function. Targeted mass spectrometry analysis showed that ACMSD enzyme activity is increased in diabetic patients, which increases the risk of heart diastolic dysfunction.
DIABETES RESEARCH AND CLINICAL PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
Yongli Hou, Yazhen Wang, Kang Tang, Yan Yang, Yiwei Wang, Ruiyan Liu, Bin Wu, Xutao Chen, Zhaoyue Fu, Feng Zhao, Lihua Chen
Summary: Diabetic cardiomyopathy (DCM) is a common complication in type I diabetic patients. This study focused on the role of CD226 in macrophage function during the progression of DCM. It was found that CD226 deficiency attenuated cardiac dysfunction in diabetic mice and decreased the proportion of CD86(+)F4/80(+) macrophages in the diabetic hearts. The study also showed that CD226 deficiency decreased macrophage glycolysis and the expression of hexokinase 2 (HK2) and lactate dehydrogenase A (LDH-A). These findings provide insights into the pathogenic roles of CD226 in DCM and potential treatment options.
Article
Endocrinology & Metabolism
Haitao Zhang, Yan Shen, Il-man Kim, Neal L. Weintraub, Yaoliang Tang
Summary: Diabetes leads to abnormal cellular metabolism in CMECs, affecting glycolysis and mitochondrial oxidative phosphorylation, resulting in increased intracellular ROS levels. Gene expression and protein levels differ between diabetic and WT CMECs. Additionally, diabetic CMECs exhibit decreased cell proliferation and increased cellular apoptosis compared to WT CMECs.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Emmi Helle, Minna Ampuja, Alexandra Dainis, Laura Antola, Elina Temmes, Erik Tolvanen, Eero Mervaala, Riikka Kivela
Summary: Cell-cell interactions between cardiac endothelial cells and cardiomyocytes are crucial for cardiac development, with co-culture leading to increased expression of genes related to vascular and cardiac development in endothelial cells. Notably, co-culture also induced expression of cardiomyocyte-specific genes in endothelial cells, highlighting the importance of EC-CM interactions during development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alberto Del Monte-Monge, Inigo Ruiz-Polo de Lara, Pilar Gonzalo, Carla Espinos-Estevez, Maria Gonzalez-Amor, Miguel de la Fuente-Perez, Maria J. Andres-Manzano, Victor Fanjul, Juan R. Gimeno, Roberto Barriales-Villa, Beatriz Dorado, Vicente Andres
Summary: Mutations in the LMNA gene can cause various cardiac diseases, including dilated cardiomyopathies. In order to investigate the potential vascular alterations in LMNA-DCM, researchers generated mice without lamin A/C in vascular smooth muscle cells, cardiac fibroblasts, and cardiomyocytes. These mice exhibited similar clinical features to human LMNA-DCM, including cardiac dysfunction and fibrosis, as well as vascular phenotypic alterations. This study provides the first evidence of vascular defects contributing to the pathophysiology of LMNA-DCM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Yunling Xuan, Chen Chen, Zheng Wen, Dao Wen Wang
Summary: In myocarditis, immune cells and their released immune regulatory factors play important roles in a complex microenvironment. However, non-immune cells and non-cardiomyocytes in the heart also act as sentinels, amplify inflammation, and interact with cardiomyocytes.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Byeong-Yun Ahn, Myong-Ho Jeong, Jung-Hoon Pyun, Hyeon-Ju Jeong, Tuan Anh Vuong, Ju-Hyeon Bae, Subin An, Su Woo Kim, Yong Kee Kim, Dongryeol Ryu, Hyun-Ji Kim, Hana Cho, Gyu-Un Bae, Jong-Sun Kang
Summary: PRMT7 plays an important role in cardiac hypertrophy and fibrosis. Overexpression of PRMT7 can alleviate cellular hypertrophic response, while depletion of PRMT7 exacerbates hypertrophic response. Transcriptome analysis reveals altered gene expression profile related to Wnt signaling pathway in PRMT7-deficient hearts.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Geriatrics & Gerontology
Xudong Zhang, Jiahui Fan, Huaping Li, Chen Chen, Yan Wang
Summary: CD36, also known as scavenger receptor B2, plays multiple roles in lipid metabolism, inflammatory signaling, oxidative stress, and energy reprogramming. It acts as a receptor that interacts with various ligands and activates specific downstream signaling pathways. CD36 signaling is closely related to diabetic cardiomyopathy, presenting a promising strategy for its treatment based on recent basic research and clinical findings.
Article
Cardiac & Cardiovascular Systems
Kishal Lukhna, Derek J. Hausenloy, Abdelbagi Sidahmed Ali, Abdullah Bajaber, Alistair Calver, Arthur Mutyaba, Awad Abdalla Mohamed, Brian Kiggundu, Chishala Chishala, Ebrahim Variava, Ehab Ali Elmakki, Elijah Ogola, Eltayeb Hamid, Emmy Okello, Isam Gaafar, Keiran Mwazo, Makoali Makotoko, Mergan Naidoo, Mohamed Elhadi Abdelhameed, Motasim Badri, Nasief van der Schyff, Omaima Abozaid, Paul Xafis, Sara Giesz, Trevor Gould, Waldo Welgemoed, Malcolm Walker, Mpiko Ntsekhe, Derek M. Yellon
Summary: Despite evidence of myocardial infarct size reduction in animal studies, remote ischaemic conditioning (RIC) failed to improve clinical outcomes in the large CONDI-2/ERIC-PPCI trial. The RIC-AFRICA trial aims to investigate whether RIC can improve clinical outcomes in higher-risk STEMI patients in environments with limited access to early reperfusion or PPCI.
CARDIOVASCULAR DRUGS AND THERAPY
(2023)
Review
Oncology
Yali Deng, Doan T. M. Ngo, Jessica K. Holien, Jarmon G. Lees, Shiang Y. Lim
Summary: Drp1 inhibition holds great potential as a targeted mitochondrial therapy for doxorubicin-induced cardiotoxicity, which is a significant challenge in clinical practice.
CURRENT ONCOLOGY REPORTS
(2022)
Article
Health Care Sciences & Services
Raju Maiti, Jialiang Li, Priyam Das, Xueqing Liu, Lei Feng, Derek J. Hausenloy, Bibhas Chakraborty
Summary: In this article, a linear combination method is proposed to improve the overall diagnostic accuracy by combining results from multiple diagnostic tests. The method involves a smooth approximation of the empirical Hyper-volume Under Manifolds and employs efficient gradient-based algorithms for better solutions. Simulation study and application to real medical datasets demonstrate the effectiveness of the proposed method.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2023)
Article
Biology
Ying-Hwey Nai, Xiaomeng Wang, Julian Gan, Cheryl Pei Ling Lian, Ryan Fraser Kirwan, Forest Su Lim Tan, Derek J. Hausenloy
Summary: The study aimed to optimize DWI image acquisition and analysis protocols in calf muscles by investigating the effects of different model-fitting methods, image quality, and use of high b-value and constraints on POIs. The optimized models showed that ADC and Dd were reliable for calf diffusion evaluation, with CVs of 7.22% and 4.09%.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jiang-Hui Wang, Daniel Urrutia-Cabrera, Jarmon G. Lees, Santiago Mesa Mora, Tu Nguyen, Sandy S. C. Hung, Alex W. Hewitt, Shiang Y. Lim, Thomas L. Edwards, Raymond C. B. Wong
Summary: Age-related macular degeneration (AMD) is a blinding disease characterized by dysfunction of the retinal pigmented epithelium (RPE). This study developed a human RPE model using CRISPRi technology, providing a useful tool for functional studies of AMD-associated genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Fan Yu, Shuo Cong, En Ping Yap, Derek J. Hausenloy, Chrishan J. Ramachandra
Summary: Ischemic heart disease is a major cause of heart failure and has a significant impact on global health. The limited regenerative capacity of cardiomyocytes poses challenges for heart repair. Recent research has shown that cardiac metabolism plays a crucial role in heart regeneration and modulating this metabolism could promote regeneration. However, there is a lack of mechanistic understanding in this field, hindering the development of effective therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Health Care Sciences & Services
Nicholas W. S. Chew, Bryan Chong, Si Min Kuo, Jayanth Jayabaskaran, Mingshi Cai, Huili Zheng, Rachel Goh, Gwyneth Kong, Yip Han Chin, Syed Saqib Imran, Michael Liang, Patrick Lim, Thon Hon Yong, Boon Wah Liew, Pow Li Chia, Hee Hwa Ho, David Foo, Deanna Khoo, Zijuan Huang, Terrance Chua, Jack Wei Chieh Tan, Khung Keong Yeo, Derek Hausenloy, Hui Wen Sim, Jieli Kua, Koo Hui Chan, Poay Huan Loh, Toon Wei Lim, Adrian F. Low, Ping Chai, Chi Hang Lee, Tiong Cheng Yeo, James Yip, Huay Cheem Tan, Mamas A. Mamas, Stephen J. Nicholls, Mark Y. Chan
Summary: The incidence of AMI is projected to continue rising, with overweight/obesity emerging as the fastest-growing metabolic risk factor and the leading risk factor for AMI-related mortality. Individuals of Indian and Malay descent bear a disproportionate burden of overweight/obesity incidence and AMI-related mortality.
LANCET REGIONAL HEALTH-WESTERN PACIFIC
(2023)
Article
Biochemistry & Molecular Biology
Gerd Heusch, Ioanna Andreadou, Robert Bell, Edoardo Bertero, Hans-Erik Botker, Sean M. Davidson, James Downey, Philip Eaton, Peter Ferdinandy, Bernard J. Gersh, Mauro Giacca, Derek J. Hausenloy, Borja Ibanez, Thomas Krieg, Christoph Maack, Rainer Schulz, Frank Sellke, Ajay M. Shah, Holger Thiele, Derek M. Yellon, Fabio Di Lisa
Summary: This review summarizes the roles of reactive oxygen species (ROS) in myocardial ischemia/reperfusion injury and cardioprotection. It emphasizes the need for cardioprotection beyond rapid reperfusion in acute myocardial infarction and discusses the pathomechanisms of myocardial ischemia/reperfusion, different modes of cell death in myocardial infarction, and interventions to protect the ischemic/reperfused myocardium. The second part focuses on ROS, providing an overview of molecular and cellular mechanisms involved in ischemia/reperfusion injury.
Article
Medicine, General & Internal
Vikram Sharma, Huili Zheng, Luciano Candilio, Jennifer M. Nicholas, Tim Clayton, Derek M. Yellon, Heerajnarain Bulluck, Derek J. Hausenloy
Summary: Baseline and post-operative elevations in high-sensitivity troponin T are independently associated with 1-year all-cause mortality in cardiac surgery patients. An elevation in hs-TnT ≥50 x URL at 24 hours post-surgery is the optimal threshold to define prognostically significant peri-operative myocardial injury.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Tu Nguyen, Daniel Urrutia-Cabrera, Luozixian Wang, Jarmon G. Lees, Jiang-Hui Wang, Sandy S. C. Hung, Alex W. Hewitt, Thomas L. Edwards, Sam McLenachan, Fred K. Chen, Shiang Y. Lim, Chi D. Luu, Robyn Guymer, Raymond C. B. Wong
Summary: This study generated a POLDIP2 knockout cell line in human retinal pigment epithelium cells using CRISPR/Cas technology. It found a link between POLDIP2 and age-related macular degeneration (AMD), and showed that the knockout of POLDIP2 does not affect cell proliferation, cell viability, phagocytosis, and autophagy. RNA sequencing revealed significant changes in genes related to immune response, complement activation, oxidative damage, and vascular development in the POLDIP2 knockout cells. Additionally, the study demonstrated that loss of POLDIP2 reduces mitochondrial superoxide levels and upregulates mitochondrial superoxide dismutase SOD2 in ARPE-19 cells, supporting a potential role of POLDIP2 in regulating oxidative stress in AMD pathology.
Article
Cardiac & Cardiovascular Systems
Sauri Hernandez-Resendiz, Aishwarya Prakash, Sze Jie Loo, Martina Semenzato, Kroekkiat Chinda, Gustavo E. Crespo-Avilan, Linh Chi Dam, Shengjie Lu, Luca Scorrano, Derek J. Hausenloy
Summary: Acute myocardial ischemia-reperfusion injury is a crucial factor in myocardial infarction and heart failure. Researchers have found that therapeutic strategies aimed at preserving mitochondrial shape and function may help protect the heart and improve health outcomes in patients with acute myocardial infarction.
BASIC RESEARCH IN CARDIOLOGY
(2023)