期刊
CARBOHYDRATE POLYMERS
卷 280, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.119001
关键词
Angelica sinensis; Insulin resistance; Polysaccharide; Type 2 diabetes; Structure
资金
- National Natural Science Foundation of China [81473329, 81173513]
- Science and Technology Department of Shaanxi Province of China [2019ZDLSF04-03-01, 2019PT23]
- Scientific Research Project of Shaanxi Administration of Traditional Chinese Medicine [2019-GJ-JC012]
- Special Foundation for Talents of Northwest AF University [2452017335]
This study identified two novel homogeneous polysaccharides, APS-1I and APS-2II, from Angelica sinensis that bind to RAGE and have effects on insulin resistance. APS-1I showed better effects on glucose absorption and IRS signaling, and improved insulin resistance in diabetic rats.
This study found two novel homogeneous polysaccharides from Angelica sinensis, APS-1I and APS-2II, binding to RAGE with a dissociation constant of 2.02 +/- 0.2 and 85.92 +/- 0.2 mu M, respectively. APS-1I is a 17.0 kDa heteropolysaccharide, whose backbone is composed of alpha-1,6-Glcp, alpha-1,3,6-Glcp, alpha-1,2-Glcp, alpha-1,4-Galp, and alpha-1,3-Rhap, and whose two branches contain alpha-1,3,5-Araf, alpha-1,3-Araf, andp-1,4-Glcp. APS-2II is a 10.0 kDa linear glucan, that contains alpha-1,6-Glcp, alpha-1,3-Glcp, alpha-1,2-Glcp, and alpha-T-Glcp. In vitro, APS-1I demonstrated better promotion on glucose absorption and stronger repression on p-IRS-1 (Ser307), p-IRS-2 (Ser731), p-JNK, and p-P38 than APS-2II in insulin resistance (IR)-HepG2 cells. Furthermore, APS-1I treatment couldn't further decrease the inhibition on the phosphorylation of JNK and P38 produced by RAGE siRNA in IR-HepG2 cells. In vivo, APS-1I markedly improved IR and reversed the livers RAGE-JNK/p38-IRS signaling in highfat-diet and streptozotocin-induced diabetic rats, suggesting that APS-1I could be a potential agent for improving IR in type 2 diabetes.
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