CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome

The Algal Chloroplast as a Testbed for Synthetic Biology Designs Aimed at Radically Rewiring Plant Metabolism

(2021) Harry O. Jackson et al.   Frontiers in Plant Science

The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis

(2020) Shreya Agrawal et al.   PLANT PHYSIOLOGY

Current advances in the algae-made biopharmaceuticals field

(2020) Sergio Rosales-Mendoza et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics

(2019) Patai Charoonnart et al.   Scientific Reports

Use of the ptxD gene as a portable selectable marker for chloroplast transformation in Chlamydomonas reinhardtii

(2019) José M. Sandoval-Vargas et al.   MOLECULAR BIOTECHNOLOGY

Plastid transformation and its application in metabolic engineering

(2018) Paulina Fuentes et al.   CURRENT OPINION IN BIOTECHNOLOGY

High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates

(2018) Sean D. Gallaher et al.   PLANT JOURNAL

Intercistronic expression elements (IEE) from the chloroplast of Chlamydomonas reinhardtii can be used for the expression of foreign genes in synthetic operons

(2018) Karla S. Macedo-Osorio et al.   PLANT MOLECULAR BIOLOGY

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

(2018) Pierre Crozet et al.   ACS Synthetic Biology

Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering

(2018) George M Taylor et al.   NUCLEIC ACIDS RESEARCH

Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory

(2018) Yong Min Kwon et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism

(2017) Marina Cavaiuolo et al.   NUCLEIC ACIDS RESEARCH

Recent achievements obtained by chloroplast transformation

(2017) Muhamed Adem et al.   Plant Methods

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

(2016) Thanyanan Wannathong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Towards developing algal synthetic biology

(2016) M. A. Scaife et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Seamless editing of the chloroplast genome in plants

(2016) Elena Martin Avila et al.   BMC PLANT BIOLOGY

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

(2015) Javier A. Gimpel et al.   ACS Synthetic Biology

Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression

(2014) Rosanna E. B. Young et al.   PLANT JOURNAL

Fusion protein linkers: Property, design and functionality

(2012) Xiaoying Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Synthetic Oligonucleotide Libraries Reveal Novel Regulatory Elements in Chlamydomonas Chloroplast mRNAs

(2012) Elizabeth A. Specht et al.   ACS Synthetic Biology

A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii

(2011) Janette Kropat et al.   PLANT JOURNAL

Molecular Factors Affecting the Accumulation of Recombinant Proteins in the Chlamydomonas reinhardtii Chloroplast

(2010) Anna T. Coragliotti et al.   MOLECULAR BIOTECHNOLOGY