期刊
BIOSENSORS & BIOELECTRONICS
卷 199, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113866
关键词
SARS-CoV-2; COVID-19; Serologic detections; Graphene; IgG; Biosensor
类别
资金
- Sao Paulo Research Foundation (FAPESP) [17/20493-2, 18/11071-0, 20/04796-8, 20/06752-8, 20/05030-1, 18/22214-6, 19/153331 e 19/12053-8]
- Coordinating Agency for Advanced Training of Graduate Personnel (CAPES), Metabolomics and Diagnostic Research Network of Covid-19 (MeDiCo Network), CAPES-Brazil [88881.504532/2020-01, 88887.511448/2020-00]
- National Council of Scientific and Technological Development (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [17/20493-2, 18/22214-6, 20/04796-8] Funding Source: FAPESP
The article introduces a graphene-based Electrical-Electrochemical Vertical Device (EEVD) point-of-care biosensor for serologic COVID-19 diagnosis. EEVD demonstrates advantages such as short analysis time (15 min), simple sample preparation, and a low limit of detection (1.0 pg mL(-1)). It meets the principles of robustness and accuracy, making it an ideal choice for pandemic control strategies.
The outbreak of COVID-19 pandemics highlighted the need of sensitive, selective, and easy-to-handle biosensing devices. In the contemporary scenario, point-of-care devices for mass testing and infection mapping within a population have proven themselves as of primordial importance. Here, we introduce a graphene-based Electrical-Electrochemical Vertical Device (EEVD) point-of-care biosensor, strategically engineered for serologic COVID-19 diagnosis. EEVD uses serologic IgG quantifications on SARS-CoV-2 Receptor Binding Domain (RBD) bioconjugate immobilized onto device surface. EEVD combines graphene basal plane with high charge carrier mobility, high conductivity, low intrinsic resistance, and interfacial sensitivity to capacitance alterations. EEVD application was carried out in real human serum samples. Since EEVD is a miniaturized device, it requires just 40 mu L of sample for a point-of-care COVID-19 infections detection. When compared to serologic assays such ELISA and other immunochromatographic methods, EEVD presents some advantages such as time of analyses (15 min), sample preparation, and a LOD of 1.0 pg mL(-1). We glimpse that EEVD meets the principles of robustness and accuracy, desirable analytic parameters for assays destined to pandemics control strategies.
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