期刊
BIOSENSORS & BIOELECTRONICS
卷 213, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114446
关键词
PCN-224; Oxidase-like activity; Glutathione; Colorimetry; Detection
类别
资金
- Natural Science Foundation of Hebei Province [B2017203229]
- Hebei Education Department of China [QN2022124]
- Key Program of Hebei University of Environmental Engineering [2020ZRZD02]
- Talent Training Programs of Hebei Province [A201901068, A201901069]
- Scientific Research Key Projects of Education Department of Hebei Province [ZD2019024]
- Science and Technology Support Program of Qinhuangdao [202101A007]
In this study, a simple colorimetric method for detecting glutathione (GSH) was developed using a manganese ion modified porphyrin metal-organic framework (PCN-224-Mn). This method showed a wide linear detection range and a low detection limit, indicating great potential as a biosensor.
The selective detection of glutathione (GSH) has been used as important colorimetric probe for human health. Herein, we used a facile method to synthesize manganese ions modified porphyrin metal-organic framework (PCN-224-Mn) with a size of 125.7 +/- 14.2 nm and zeta potential of -3.9 +/- 0.5 mV. We showed that PCN-224-Mn catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the absence of H2O2, resulting in a blue-colored oxidized TMB (oxTMB) that exhibits oxidase-like activity. Furthermore, a simple colorimetric detection method for GSH was developed based on the oxidase-like activity of PCN-224-Mn. This method shows wide linear detection range of 0.5-60 mu M for GSH with a much lower detection limit of 0.233 mu M. Finally, the recovery of colorimetric sensor of PCN-224-Mn suggests its great potential as a biosensor. As the catalytically active site, the manganese porphyrin unit plays a major role in the oxidase-like property and detection ability of PCN-224-Mn. Our data suggest that GSH detection method using PCN-224-Mn has great potential in multiple applications in the future.
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