Article
Chemistry, Analytical
Ruo Yuan, Ya-Qin Chai, Jia-Hang Li, Jia-Li Liu, Xiao-Long Zhang, Xiao-Chun Zhu
Summary: A novel ultrasensitive electrochemiluminescence (ECL) biosensor was developed using 2D Co3O4 nanosheets as a coreaction accelerator for the detection of miRNA-21. The Co3O4 nanosheets promoted the decomposition of H2O2 to generate more O2.-, which enhanced the ECL signals by reacting with luminol. Additionally, the biosensor achieved ultrasensitive detection of miRNA-21 through the self-assembly of highly ordered rolling DNA nanomachines.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yu-Ting Yang, Jia-Li Liu, Man-Fei Sun, Ruo Yuan, Ya-Qin Chai
Summary: A novel electrochemiluminescence biosensor based on MnS:CdS@ZnS QDs was developed for ultrasensitive detection of miRNA let-7a. The biosensor exhibited high stability and excellent sensitivity, with a detection limit as low as 4.1 aM.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaoli Zhang, Ying Zhou, Yaqin Chai, Ruo Yuan
Summary: A novel DNA cascade amplification method was developed as a signal amplifier for constructing a highly specific and ultrasensitive electrochemiluminescence (ECL) biosensor for detecting HIV DNA fragments. The RCA products anchored on the Pt-modified GCE via Pt-N bond allows for capturing Ru-labeled ssDNA as the ECL signal tag, achieving highly specific and ultrasensitive detection of the target.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Weijia Sun, Nuo Zhang, Xiang Ren, Dan Wu, Yue Jia, Qin Wei, Huangxian Ju
Summary: This study successfully prepared a biosensor for detecting miRNA-21, a potential biomarker for breast cancer. The biosensor coated the electrode with a mesoporous silica encapsulated self-enhanced emitter, enabling sensitive detection of miRNA-21. The method demonstrated good accuracy and reproducibility, providing a new approach for ultra-sensitive detection of miRNA and promoting breast cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Nan Zhang, Zi-Yuan Zhao, Hang Gao, Yue Yu, Jian-Bin Pan, Hong-Yuan Chen, Jing-Juan Xu
Summary: An ECL sensing platform for ultrasensitive detection of miRNA-122 was developed using novel emitters of polymer dots and signal amplification strategies, achieving a wide linear range and low detection limit. The biosensor combines DSN-assisted target recycling and RCA strategies, ultimately capturing ferrocene modified DNA on the electrode to quench the ECL signal of the Pdots.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Emily Kerr, Ryan Farr, Egan H. Doeven, Yi Heng Nai, Richard Alexander, Rosanne M. Guijt, Beatriz Prieto-Simon, Paul S. Francis, Megan Dearnley, David J. Hayne, Luke C. Henderson, Nicolas H. Voelcker
Summary: The sensor demonstrated in this study combines an ECL molecular beacon with magnetic bead based extraction of the miRNA target sequence to provide a sensitive and portable solution for miRNA-21 detection. The sensor does not require intercalating labels, reporter probes, added enzymes or hairpin primers for target amplification, offering a simple and robust strategy for miRNA detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Mei-Ling Lu, Wei Huang, Shuzhen Gao, Jia-Ling Zhang, Wen-Bin Liang, Yan Li, Ruo Yuan, Dong-Rong Xiao
Summary: In this study, a pyrene-based hydrogen-bonded organic framework (Py-HOF) with remarkable electrochemiluminescence (ECL) performance was prepared. The enhanced ECL emission of Py-HOF was attributed to the assembly of H4TBAPy into J-aggregates through hydrogen bonding and the high porosity of Py-HOF. Py-HOF was successfully employed as a probe for an ultrasensitive ECL sensor, demonstrating satisfactory detection performance.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shuangtian Dong, Dongyang Wang, Xuwen Gao, Li Fu, Jingna Jia, Yuqi Xu, Bin Zhang, Guizheng Zou
Summary: This study proposes a method to achieve chemiluminescence with different wavelengths and intensities by adjusting triggering agents, which shows promising potential for applications in immunoassays and other fields.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Qian Wang, Zuhao Zhang, Lu Zhang, Yunqing Liu, Li Xie, Shenguang Ge, Jinghua Yu
Summary: In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a Co3O4@Au nanoemitter is presented for the in vitro monitoring of miRNA-141. The biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biophysics
Shuang He, Pei Zhang, Jing Sun, Yu Ji, Chusen Huang, Nengqin Jia
Summary: A novel potential-resolved molecularly imprinted electrochemical luminescence immunosensor has been developed for the sensitive detection of markers of acute myocardial infarction. The sensor utilizes a cost-effective and robust molecularly imprinted polymer to specifically capture the biomarkers and generate distinct electrochemical luminescence signals. The sensor exhibits a wide linear range, low detection limit, and excellent performance.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Hanbiao Liu, Zhen Liu, Jiangle Yi, Dongxiao Ma, Fangquan Xia, Dong Tian, Changli Zhou
Summary: A novel hollow copper-cobalt MOF was synthesized and a dual-signal biosensor based on ECL signals was successfully constructed, capable of simultaneous detection of acetamiprid and malathion with good sensitivity, specificity, repeatability, and stability in apple and tomato samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Gui Zhang, Qian Han, Li Song, Pingkun Liu, Guangrong Kuang, Yingzi Fu
Summary: The enthusiasm for green chemistry among chemists has increased, leading to the synthesis of plant leaf-derived carbon dots and oxidized carbon dots. The oxidized maple CDP showed promising results as a green source luminophore and was used for the ultrasensitive detection of miRNA-21. This work provides a new opportunity for synthesizing green source luminophores and detecting various trace miRNA species.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Di Liu, Guomin Yang, Xiaolong Zhang, Shihong Chen, Ruo Yuan
Summary: This study developed a novel potential-regulated ECL ratiometric strategy for miRNA detection based on the opposite effect of H2O2 on two different ECL emissions from PFO NPs, without the need for RET and exogenous co-reactant. The strategy showed promise for bioanalysis and achieved a low detection limit of 17 aM for miRNA-155.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Wei Lai, Yi Liang, Xinyuan Mao, Kaifa Xue, Chunsun Zhang
Summary: This paper presents a cloth-based single-working-electrode electrochemiluminescence (SWE-ECL) sensor for simultaneous detection of multiple diabetes and diabetic complications (DDC) markers. The sensor simplifies the configuration of traditional sensors by distributing three independent ECL cells on the cloth. The modification processes and ECL reactions occur at the back of the SWE, avoiding adverse effects caused by human intervention. The sensor shows good sensitivity, specificity, and reproducibility, and has potential for actual application in complex human serum samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Ying Zhou, Xiaoyu Shi, Liang Ma, Yaqin Chai, Ruo Yuan
Summary: In this study, an electrochemiluminescence (ECL) biosensor is developed for simultaneous and ultrasensitive detection of glutathione (GSH) and copper ion (Cu2+), using thiol stabilized gold nanoclusters (Au NCs) as a highly efficient ECL emitter. The study reveals that thiol-Au NCs exhibit exceptional ECL performance over amino-Au NCs due to fast electron transfer. The ECL biosensor achieves trace detection of GSH with a detection limit of 148 pM and Cu2+ with a detection limit of 91.5 fM, providing a definite direction for obtaining ultra-bright ECL metal NCs for multiple biomarker testing and expanding their application in clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)