Engineering of a nitrilase through consensus sequence analysis and conserved site substitution to improve its thermostability and activity

Efficient biosynthesis of 1-cyanocyclohexaneacetic acid using a highly soluble nitrilase by N-terminus modification of novel peptide tags

(2021) Neng Xiong et al.   BIOCHEMICAL ENGINEERING JOURNAL

Immobilization of Escherichia coli cells harboring a nitrilase with improved catalytic properties though polyethylenemine-induced silicification on zeolite

(2021) Neng Xiong et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Engineering a Pichia pastoris nitrilase whole cell catalyst through the increased nitrilase gene copy number and co-expressing of ER oxidoreductin 1

(2020) Qi Shen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Upscale production of (R)-mandelic acid with a stereospecific nitrilase in an aqueous system

(2020) Xin-Hong Zhang et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Expanding the repertoire of nitrilases with broad substrate specificity and high substrate tolerance for biocatalytic applications

(2020) Avinash Vellore Sunder et al.   PROCESS BIOCHEMISTRY

Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6

(2019) Dejian Liu et al.   BIOTECHNOLOGY LETTERS

Thermophiles and the applications of their enzymes as new biocatalysts

(2019) Joaquín Atalah et al.   BIORESOURCE TECHNOLOGY

Lipase immobilized to a short alkyl chain-containing zwitterionic polymer grafted on silica nanoparticles: Moderate activation and significant increase of thermal stability

(2019) Chunyu Zhang et al.   BIOCHEMICAL ENGINEERING JOURNAL

A comparative multivariate analysis of nitrilase enzymes: an ensemble based computational approach

(2019) Priya Kumari et al.   COMPUTATIONAL BIOLOGY AND CHEMISTRY

Bridges to Stability: Engineering Disulfide Bonds Towards Enhanced Lipase Biodiesel Synthesis

(2019) Shalev Gihaz et al.   ChemCatChem

Efficient Chemoenzymatic Synthesis of Optically Active Pregabalin from Racemic Isobutylsuccinonitrile

(2019) Qin Zhang et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

How metal cofactors drive dimer–dodecamer transition of the M42 aminopeptidase TmPep1050 of Thermotoga maritima

(2019) Raphaël Dutoit et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Efficient biocatalytic synthesis of nicotinic acid by recombinant nitrilase via high density culture

(2018) Jin-Song Gong et al.   BIORESOURCE TECHNOLOGY

Engineering the residues on “A” surface and C-terminal region to improve thermostability of nitrilase

(2018) Zhe Xu et al.   ENZYME AND MICROBIAL TECHNOLOGY

Highly efficient production of 1-cyanocyclohexaneacetic acid by cross-linked cell aggregates (CLCAs) of recombinant E. coli harboring nitrilase gene

(2018) Shu-Ping Zou et al.   PROCESS BIOCHEMISTRY

Highly efficient conversion of 1-cyanocycloalkaneacetonitrile using a “super nitrilase mutant”

(2018) Zhe Xu et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Cloning, overexpression, and characterization of a thermostable nitrilase from an Antarctic Pyrococcus sp.

(2017) Ma Ángeles Cabrera et al.   EXTREMOPHILES

High-throughput screening methods for nitrilases

(2016) Ya-Ping Xue et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance

(2016) Sarah A. Sonbol et al.   BMC BIOTECHNOLOGY

Probing an Interfacial Surface in the Cyanide Dihydratase from Bacillus pumilus, A Spiral Forming Nitrilase

(2016) Jason M. Park et al.   Frontiers in Microbiology

Bacillus pumilus Cyanide Dihydratase Mutants with Higher Catalytic Activity

(2016) Mary A. Crum et al.   Frontiers in Microbiology

Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid

(2015) Hualei Wang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Directed Evolution and Mutant Characterization of Nitrilase from Rhodococcus rhodochrous tg1-A6

(2015) Hui Luo et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Nitrilase-catalyzed conversion of (R,S)-mandelonitrile by immobilized recombinantEscherichia colicells harboring nitrilase

(2015) Xin-Hong Zhang et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Chemoenzymatic synthesis of gabapentin by combining nitrilase-mediated hydrolysis with hydrogenation over Raney-nickel

(2015) Ya-Ping Xue et al.   CATALYSIS COMMUNICATIONS

C-terminal hybrid mutant ofBacillus pumiluscyanide dihydratase dramatically enhances thermal stability and pH tolerance by reinforcing oligomerization

(2015) M.A. Crum et al.   JOURNAL OF APPLIED MICROBIOLOGY

Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization

(2014) Heng Li et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Improvement of Alcaligenes faecalis Nitrilase by Gene Site Saturation Mutagenesis and Its Application in Stereospecific Biosynthesis of (R)-(−)-Mandelic Acid

(2014) Zhi-Qiang Liu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp. PCC6803

(2014) Lujia Zhang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Activity improvement of a regioselective nitrilase from Acidovorax facilis and its application in the production of 1-(cyanocyclohexyl) acetic acid

(2014) Xin-Hong Zhang et al.   PROCESS BIOCHEMISTRY

Engineering proteins for thermostability through rigidifying flexible sites

(2013) Haoran Yu et al.   BIOTECHNOLOGY ADVANCES

Innovation by homologous recombination

(2013) Devin L Trudeau et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Efficient production of (R)-(−)-mandelic acid in biphasic system by immobilized recombinant E. coli

(2013) Kefeng Ni et al.   JOURNAL OF BIOTECHNOLOGY

Comparative analysis of amino acid sequences from mesophiles and thermophiles in respective of carbon–nitrogen hydrolase family

(2013) Sarita Devi et al.   3 Biotech

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY