Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase-negative staphylococci (CoNS) are a possible alternative pathogen. This case-control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain-level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases.

Automated summary

This case-control study examined the role of CoNS in mastitis and found that the majority of CoNS isolates were Staphylococcus epidermidis, with no specific profile associated with mastitis cases.