4.4 Article

Biological characterization and genomic analysis of Acinetobacter baumannii phage BUCT628

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ARCHIVES OF VIROLOGY
卷 167, 期 6, 页码 1471-1474

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SPRINGER WIEN
DOI: 10.1007/s00705-022-05425-7

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  1. Funds for First-class Discipline Construction [XK1805, XK1803-06]
  2. National Key Research and Development Program of China [2018YFA0903000, 2020YFC2005405, 2020YFA0712100, 2020YFC0840805, 19SWAQ06, 20SWAQX27, 20SWAQK22]
  3. Inner Mongolia Key Research and Development Program [2019ZD006]
  4. NSFC-MFST project (China-Mongolia) [31961143024]
  5. Fundamental Research Funds for Central Universities [BUCTRC201917, BUCTZY2022]

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In this study, the Acinetobacter phage BUCT628 was isolated from hospital wastewater. The genome sequence of BUCT628 showed high similarity with Acinetobacter phage Bphi-R2919. The genome of BUCT628 is a linear dsDNA molecule with a length of 44,935bp and contains 86 open reading frames.
Acinetobacter baumannii is an opportunistic pathogen that is resistant to the most commonly used antibiotics. In this study, the Acinetobacter phage BUCT628 was isolated from hospital wastewater. BLASTn analysis showed that the genome sequence of BUCT628 shared 89.76% identity with 66% query coverage with that of Acinetobacter phage Bphi-R2919. Genome sequencing showed that the BUCT628 genome is a 44,935-bp linear dsDNA molecule with 37.5% G+C content and 86 open reading frames (ORFs), and no tRNAs were identified.

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