Article
Biotechnology & Applied Microbiology
Allison J. Sherier, August E. Woerner, Bruce Budowle
Summary: Microbial DNA shed from human skin can be distinctive to its host, aiding in individualizing donors of forensic biological evidence. Genetic differentiation may be more suitable for individual identification.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yu-An Kuo, Cheulhee Jung, Yu-An Chen, Hung-Che Kuo, Oliver S. Zhao, Trung D. Nguyen, James R. Rybarski, Soonwoo Hong, Yuan- Chen, Dennis C. Wylie, John A. Hawkins, Jada N. Walker, Samuel W. J. Shields, Jennifer S. Brodbelt, Jeffrey T. Petty, Ilya J. Finkelstein, Hsin-Chih Yeh
Summary: The study revealed that the nucleobases at positions 7-12 of the 18-nucleotide-long activator are critical to creating bright NCBs, while positions 4-6 and 2-4 are hotspots to generate yellow-orange and red POTs.
ADVANCED MATERIALS
(2022)
Article
Genetics & Heredity
Xuewen Wang, Meng Huang, Bruce Budowle, Jianye Ge
Summary: The article introduces a novel algorithm and software program, TRcaller, for genotyping TR alleles from long-read sequencing data. TRcaller provides higher accuracy and faster speed in detecting TR alleles from both whole genome and targeted sequences generated from multiple sequencing platforms.
FRONTIERS IN GENETICS
(2023)
Article
Biochemical Research Methods
James Gooch, Sireethorn Tungsirisurp, Hayley Costanzo, Richard Napier, Nunzianda Frascione
Summary: The study selected ssDNA aptamers against human sperm cells using Cell-SELEX and massively parallel sequencing technologies, revealing promising aptamer candidates that selectively bind sperm cells. These aptamers have the potential to increase the efficiency of sexual offence investigations by facilitating sperm detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Aideen M. McInerney-Leo, Emma L. Duncan
Summary: There have been two major eras in gene discovery history, from linkage analysis to massively parallel sequencing (MPS). MPS has greatly accelerated the identification of disease genes, reducing the time needed to determine disease genes from years to weeks.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Genetics & Heredity
Jun Zhang, Wenli Liu, Halimureti Simayijiang, Ping Hu, Jiangwei Yan
Summary: Recent advances in next-generation sequencing technologies and improvements in bioinformatics have expanded the scope of microbiome analysis as a forensic tool. Microbiome research is concerned with the study of the compositional profile and diversity of microbial flora as well as the interactions between microbes, hosts, and the environment. It has opened up many new possibilities for forensic analysis.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2023)
Review
Microbiology
Suleiman Aminu, Abdulaziz Ascandari, Meriem Laamarti, Nour El Houda Safdi, Achraf El Allali, Rachid Daoud
Summary: This review provides an overview of the use of whole genome sequencing (WGS) in microbiome research, highlighting its importance in unraveling the diversity and complexity of microbial communities in different environments. It discusses the relationship between the gut microbiome and human health, the impact of human activities on microbial communities, and the investigation of microbiomes in various animal species and plant tissues.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Review
Genetics & Heredity
Erin M. Gorden, Kimberly Sturk-Andreaggi, Charla Marshall
Summary: Hybridization capture technology has gained attention in the forensic field for its ability to process degraded DNA fragments and enrich mitochondrial DNA or nuclear genome polymorphisms through massively parallel sequencing. As the technology advances, hybridization capture becomes increasingly important in analyzing DNA from aged and degraded remains.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2021)
Article
Genetics & Heredity
Jordan Beasley, Guy Shorrock, Rita Neumann, Celia A. May, Jon H. Wetton
Summary: Birds of prey have been persecuted for centuries, and their DNA has been used for forensic analysis. This study developed 14 new STR markers for identifying historical peregrine falcon samples, providing quicker and more efficient results compared to previous methods.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2021)
Review
Chemistry, Multidisciplinary
Junyun Cheng, Gaole Lin, Tianhao Wang, Yunzhu Wang, Wenbo Guo, Jie Liao, Penghui Yang, Jie Chen, Xin Shao, Xiaoyan Lu, Ling Zhu, Yi Wang, Xiaohui Fan
Summary: CRISPR-based genetic screening is a powerful approach for unbiased functional genomics research. Single-cell CRISPR screening techniques allow the analysis of regulatory networks in complex biological systems at unprecedented resolution. These methods involve perturbing cells en masse using a pooled CRISPR library and high-content phenotyping.
Article
Obstetrics & Gynecology
Songchang Chen, Xuyang Yin, Sijia Zhang, Jun Xia, Ping Liu, Pingyuan Xie, Huijuan Yan, Xinming Liang, Junyu Zhang, Yiyao Chen, Hongjun Fei, Lanlan Zhang, Yuting Hu, Hui Jiang, Ge Lin, Fang Chen, Chenming Xu
Summary: WGS offers a cost-effective and reliable approach for genome-wide haplotyping and PGT for monogenic disorders, aneuploidy, and structural rearrangements without the need for additional family members, showing 100% concordance with traditional PGT methods.
HUMAN REPRODUCTION
(2021)
Article
Immunology
Yuyang Hou, Meng Zhang, Qiannan Jiang, Yuping Yang, Jiang Liu, Ke Yuan, Zheng Sun, Xiuxiang Liu
Summary: We used a newly developed ultra-sensitive metagenomic sequencing method, 2bRAD-M, to investigate the microbial signatures of central nervous system infections in neonates. By applying this method, we identified potential biomarkers that have significantly different relative abundance based on cerebrospinal fluid examination results, which can aid in the diagnosis of neonatal bacterial meningitis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Jason A. Rothman, Jenna L. Riis, Katrina R. Hamilton, Clancy Blair, Douglas A. Granger, Katrine L. Whiteson
Summary: Human oral microbial communities have significant implications for oral and systemic health due to their diversity. Understanding the differences between healthy and dysbiotic oral microbiomes is important, especially within and between families. This study investigates the changes in oral microbiome composition within individuals and the impact of factors such as environmental tobacco smoke exposure, metabolic regulation, inflammation, and antioxidant potential. The findings reveal differences in oral microbiomes between children and caregivers, with shared diversity but distinct differences, and highlight the associations between the oral microbiome and various salivary markers.
Article
Microbiology
Wataru Aoki, Masato Kogawa, Shuhei Matsuda, Keisuke Matsubara, Shintaro Hirata, Yohei Nishikawa, Masahito Hosokawa, Haruko Takeyama, Toru Matoh, Mitsuyoshi Ueda
Summary: This study developed a pipeline to analyze the genomic diversity of the rice rhizosphere microbiome at single-cell resolution. By isolating microbial cells from paddy soil and determining their genomic sequences using massively parallel whole-genome amplification, the researchers obtained a large number of genomic sequences that provided insights into microbial functions in the paddy ecosystem. This approach offers a promising platform for gaining novel insights into the roles of microbes in the rice rhizomicrobiome and developing microbial technologies for improved and sustainable rice production.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ludmila Volozonoka, Anna Miskova, Linda Gailite
Summary: This review provides insight into the performance and drawbacks of WGA techniques such as DOP-PCR, MDA, MALBAC, and hybrid techniques in PGT. The advantages of MDA-based solutions for comprehensive genome coverage and PCR-based solutions for uniformity of coverage are highlighted. The choice of WGA technique should take into consideration the influence of MPS parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Legal
Allison J. Sherier, Rachel E. Kieser, Nicole M. M. Novroski, Frank R. Wendt, Jonathan L. King, August E. Woerner, Angie Ambers, Paolo Garofano, Bruce Budowle
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Allison J. Sherier, August E. Woerner, Bruce Budowle
Summary: Microbial DNA shed from human skin can be distinctive to its host, aiding in individualizing donors of forensic biological evidence. Genetic differentiation may be more suitable for individual identification.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)