期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 414, 期 11, 页码 3529-3539出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-022-03979-8
关键词
miRNA extraction; Nucleic acid extraction; Rapid extraction; Electrical potential-assisted hybridization
资金
- National Key Research and Development Programs of China [2018YFE0113300]
- National Natural Science Foundation of China [81801264]
- Key Project of Shandong Provincial Natural Science Foundation [ZR2020KH030]
This study presents a novel strategy for miRNA extraction based on electrical potential-assisted DNA-RNA hybridization. The entire extraction process is rapid and sensitive, with the advantages of simplicity and specificity. Satisfactory results were achieved for miRNA extraction in serum.
Analysis of microRNAs (miRNAs) is important in cancer diagnostics and therapy. Conventional methods used to extract miRNA for analysis are generally time-consuming. A novel approach for rapid and sensitive extraction of miRNAs is urgently need for clinical applications. Herein, a novel strategy based on electrical potential-assisted DNA-RNA hybridization was designed for miRNA extraction. The entire extraction process was accomplished in approximately 3 min, which is much shorter than the commercial adsorption column method, at more than 60 min, or the TRIzol method, at more than 90 min. Additionally, the method offered the advantages of simplicity and specificity during the extraction process by electrical potential-assisted hybridization of single-stranded DNA and RNA. Taking let-7a as an example, satisfactory results were achieved for miRNA extraction in serum, demonstrating the applicability in miRNA nucleic acid amplification.
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