期刊
CANADIAN JOURNAL OF ZOOLOGY
卷 93, 期 9, 页码 711-720出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/cjz-2014-0181
关键词
Apis mellifera; Israeli acute paralysis virus; Dicistroviridae; mass spectrometry; proteomics
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Genome Canada
- Genome British Columbia
- British Columbia Honey Producers Association through the Boone-Hodgson-Wilkinson Fund
- Canadian Honey Council through the Canadian Bee Research Fund
- Canadian Association of Professional Apiculturists through the Canadian Bee Research Fund
- British Columbia Blueberry Council
- British Columbia Cranberry Marketing Association
- University of British Columbia
- Agri-Food Canada's Advancing Canadian Agriculture and Agri-Food (ACAAF) program
- Canada Foundation for Innovation
- British Columbia Knowledge Development Fund
- British Columbia Proteomics Network
- Canadian Institutes of Health Research Fellowship
- National Academy of Sciences
- US National Institute of Food and Agriculture AFRI grant [2010-65104-20533]
Recent declines in honey bee (Apis mellifera L., 1758) populations worldwide have spurred significant research into the impact of pathogens on colony health. The role of the Israeli acute paralysis virus (IAPV) on hive mortality has become of particular concern since being correlated with colony losses. However, the molecular interactions between IAPV and its host remain largely unknown. To investigate changes in host protein expression during IAPV infection, mass-spectrometry-based quantitative proteomics was used to compare IAPV-infected and healthy pupae. Proteins whose expression levels changed significantly during infection were identified and functional analysis was performed to determine host systems and pathways perturbed by IAPV infection. Among the A. mellifera proteins most affected by IAPV, those involving translation and the ubiquitin-proteasome pathway were most highly enriched and future investigation of these pathways will be useful in identifying host proteins required for infection. This analysis represents an important first step towards understanding the honey bee host response to IAPV infection through the systems-level analysis of protein expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据