Aureimonas galii sp nov and Aureimonas pseudogalii sp nov isolated from the phyllosphere of Galium album

Four yellow-pigmented, Gram-stain-negative, rod-shaped bacteria, strains PP-WC-4G-234(T), PP-CE-2G-454(T), PP-WC-1G-202 and PP-CC-3G-650, were isolated from the phyllosphere of Galium album. The strains shared 99.7-100% 16S rRNA gene sequence similarity but could be differentiated by genomic fingerprinting using rep-and random amplification of polymorphic DNA PCRs. Phylogenetic analysis based on the 16S rRNA gene placed the strains within the family Aurantimonadaceae with highest 16S rRNA gene sequence similarity of 97.2-97.3% to the type strain of Aureimonas phyllosphaerae. Sequence similarities to all other Aurantimonadaceae were below 97 %. The main cellular fatty acids of the strains were C-18 : 1 omega 7c as the predominant fatty acid followed by C-16 : 0 and summed feature 3 (C-16 : 1 omega 7c/C-16 : 1 omega 8c). The polyamine patterns of strains PP-WC-4G-234(T) and PP-CE-2G-454(T) contained sym-homospermidine as a major compound, and the major respiratory quinone was ubiquinone Q-10. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, three unidentified phospholipids and one unidentified lipid only detectable after total lipid staining. The DNA G+C content was 66.4, 68.9, 67.4 and 70.5 mol% for strains PP-WC-4G-234(T), PP-CE-2G-454(T), PP-WC-1G-202 and PP-CC-3G-650, respectively. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose two novel species of the genus Aureimonas, Aureimonas galii sp. nov. with PP-WC-4G-234(T) (= LMG 28655(T) = CIP 110892(T)) as the type strain and Aureimonas pseudogalii sp. nov. with PP-CE-2G-454(T) (= LMG 29411(T) = CCM 8665(T)) as the type strain and two further strains representing the same species, PP-WC-1G-202 and PP-CC-3G-650.