Article
Microbiology
Hovik Panosyan, Armine Margaryan, Nils-Kare Birkeland
Summary: Twelve thermophilic Anoxybacillus strains were isolated from a hot spring in Nagorno-Karabakh, with strain K1(T) being Gram-stain-positive, moderately thermophilic, and capable of growth within specific temperature and pH ranges. The strain showed high similarity to Anoxybacillus flavithermus DSM 2641(T) but was proposed as a separate species, Anoxybacillus karvacharensis, based on genetic analysis.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
A. A. Frolova, A. Yu. Merkel, V. V. Kevbrin, D. S. Kopitsyn, A. I. Slobodkin
Summary: An alkaliphilic, facultatively anaerobic bacterium, Sulfurospirillum tamanensis, was isolated from a terrestrial mud volcano in Russia. The bacterium showed optimum growth at 30℃ and pH 9.0, and could utilize various substrates as electron donors and acceptors. It was also capable of microaerobic growth and exhibited high similarity to Sulfurospirillum alkalitolerans.
Article
Microbiology
Seung Seob Bae, Yoon-Hee Jung, Yong Min Kwon, Dawoon Chung, Kyunghwa Baek
Summary: The bacterium strain S7(T) isolated from seawater in Korea belongs to the genus Ferrimonas and represents a novel species named Ferrimonas lipolytica sp. nov. It is a Gram-stain-negative, motile, facultatively anaerobic rod-shaped bacterium with a polar flagellum.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chinda Chhe, Ayaka Uke, Sirilak Baramee, Chakrit Tachaapaikoon, Patthra Pason, Rattiya Waeonukul, Khanok Ratanakhanokchai, Akihiko Kosugi
Summary: The newly isolated cellulolytic xylanolytic bacteria strain DA-C8 shows efficient xylan degradation within 4 days under anaerobic conditions. Phylogenetic analysis indicates that DA-C8 belongs to Paenibacillus. It exhibits high expression of xylan and cellulose degradation genes under anaerobic conditions but is repressed under aerobic conditions.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Microbiology
Shiori Hirano, Takeshi Terahara, Koji Mori, Moriyuki Hamada, Ryo Matsumoto, Takeshi Kobayashi
Summary: A novel marine bacterium, Vallitalea longa sp. nov., strain SH18-1T, was isolated from sediment near Sado Island in Japan. This strain is anaerobic, Gram-stain-negative, rod-shaped, and motile. It has a wide temperature, salt concentration, and pH range for growth. Phylogenetic analysis shows its closest related species to be Vallitalea guaymasensis Ra1766G1T. The results indicate that strain SH18-1T represents a new species of the genus Vallitalea.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Geeta Chhetri, Inhyup Kim, Taegun Seo
Summary: A new bacterium strain RG28(T) was isolated from the roots of rice plant collected from paddy fields in South Korea. It belongs to the genus Gottfriedia and has some unique physiological and chemical characteristics, as well as the ability to produce indole-acetic acid.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Noori Borhanudin, Min Yang, Andrei V. Chaplin, Jiang Li, Qiong Wang, Li-rong Dai, Ke-jia Wu, Shi-chun Ma, Boris A. Efimov, Lei Cheng
Summary: Two anaerobic, mesophilic bacteria SF3(T) and ASD5510 were isolated from human feces in different countries. Strain SF3(T) showed high sequence similarity with strain ASD5510 and a similar strain Aminipila butyrica DSM 103574(T) based on 16S rRNA gene analysis. Strain SF3(T) exhibited a Gram-stain-positive morphology and grew optimally under specific conditions. Based on phenotypic, phylogenetic, and phylogenomic evidence, strain SF3(T) represents a novel species in a novel genus named Hominibacterium faecale gen. nov., sp. nov.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hui Fan, Jiang Li, Weidong Wu, Rui Chen, Min Yang, Yinggang Zhang, Lulu Cong, Lirong Dai, Yu Deng, Lei Cheng, Shichun Ma
Summary: A Gram-stain positive bacterium JN-28T was isolated from Chinese strong-flavor liquor. It is moderately thermophilic, acidotolerant and aerotolerant anaerobic bacteria. It belongs to a novel genus and species Acidilutibacter cellobiosedens, and also proposed new families in the order Tissierellales.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Huijuan Jin, Leitao Huo, Yi Yang, Yan Lv, Jingjing Wang, Julien Maillard, Christof Holliger, Frank E. Loffler, Jun Yan
Summary: Two anaerobic bacterial isolates, ACSDCET and ACSTCE, capable of respiring tetrachloroethene (PCE), were characterized. These strains showed unique morphological and physiological characteristics, including optimum growth conditions, electron donors, and acceptors. Genomic analysis further confirmed the distinction of these strains from other Sulfurospirillum species, proposing a new species named Sulfurospirillum diekertiae sp. nov. This study provides important insights into the diversity and functionalities of PCE-respiring bacteria.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Yodai Kobayashi, Tomohiro Kawahara, Shoichiro Inoue, Noriyuki Kohda
Summary: A bacterial strain, WON2089T, was isolated from healthy Japanese adults' feces and can use mucin as the sole carbon and nitrogen source. It is closely related to Akkermansia muciniphila MucT and Akkermansia glycaniphila PytT, with 98.0% and 94.4% similarity in 16S rRNA gene sequencing, respectively. Based on phenotypic, phylogenetic and genetic characteristics, it represents a novel species of the genus Akkermansia and is named Akkermansia biwaensis sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Qing Li, Taiming Shen, Yingying Dong, Yuhui Liu, Jieliang Nong, Jiayuan Pan, Bei Fu, Qinglin Xie
Summary: Strain YB-6(T) is a Gram-stain-positive, anaerobic, spore-forming, rod-shaped bacterium that was isolated from activated sludge of an anaerobic tank at Weizhou marine oil mining wastewater treatment plant in Beihai, Guangxi, PR China. It grows at a temperature range of 25-45°C (optimum, 37°C), pH range of 4-12 (pH 7.0) and NaCl concentration of 0-7% w/v (0%). The strain can grow slowly in petroleum wastewater and remove organic carbon. Phylogenetic analysis indicates that strain YB-6(T) belongs to Clostridium cluster I and is closely related to Clostridium uliginosum CK55(T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Qi Zhang, Guohong Liu, Manik Prabhu Narsing Rao, Rong Tang, Shang Yang, Wen-Yuan Ye, Shun-Gui Zhou, Wen-Jun Li
Summary: Two Gram-stain-negative, motile, facultatively anaerobic, rod-shaped strains, FJAT-53720T and FJAT-53726, isolated from rhizosphere sediment of plant Cyperus malaccensis, belong to the genus Shewanella with unique physiological and biochemical features, such as growth at 30℃ and pH 7, specific respiratory quinones and polar lipids, and major fatty acids iso-C-15:0 and C-17:1 omega(8)c.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Marianne Quemeneur, Gael Erauso, Manon Bartoli, Celine Vandecasteele, Laura Wils, Lisa Gil, Christophe Monnin, Bernard Pelletier, Anne Postec
Summary: The novel anaerobic bacterium Alkalicella caledoniensis gen. nov., sp. nov. has a broad range of growth conditions, with the ability to grow at 25-50 degrees Celsius and pH 8.2-10.8. The bacterium contains iso-C-15:0 and C-16:0 as the major cellular fatty acids in its cellular membrane.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Anna Schnurer, Abhijeet Singh, Shaojie Bi, Wei Qiao, Maria Westerholm
Summary: A novel anaerobic bacterial strain, AMB_01T, was isolated from a high-ammonia biogas digester and characterized. The strain showed unique characteristics such as growth at a wide range of temperatures, tolerance to high ammonia concentration, and utilization of various carbon sources. Analysis of genomic data confirmed that AMB_01T represents a new species, named Miniphocaeibacter halophilus sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Yeseul Shin, Jayoung Paek, Hongik Kim, Joong-Ki Kook, Young-Hyo Chang
Summary: A novel obligately anaerobic bacterium strain, Faecalicatena absiana sp. nov., was isolated from pig farm faeces dump. Phylogenetic analysis based on gene sequences and characterization of its phenotypic and chemotaxonomic properties revealed that the strain is distinct from other known Faecalicatena species, and represents a new species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Matthias Dreier, Marco Meola, Helene Berthoud, Noam Shani, Daniel Wechsler, Pilar Junier
Summary: This study compared the performance of NGS and HT-qPCR methods in analyzing the microbial composition of cheese DNA samples. The results showed that the normalized microbial composition obtained by both methods agreed for most of the analyzed cheese samples, indicating that they can be considered as complementary and reliable research methods.
Article
Microbiology
Demosthenes P. Morales, Aaron J. Robinson, Andrew C. Pawlowski, Caitlyn Ark, Julia M. Kelliher, Pilar Junier, James H. Werner, Patrick S. G. Chain
Summary: This study evaluates the challenges in resolving bacterial-fungal interactions and proposes the use of fluorescence in situ hybridization (FISH) for microscopy imaging to obtain more conclusive evidence on the nature of specific relationships between bacteria and fungi.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Michelle Qiu Carter, Nicole Laniohan, Chien-Chi Lo, Patrick S. G. Chain
Summary: This study examines the pathogenic potential of Shiga toxin-producing Escherichia coli (STEC) O145:H28 in clinical and environmental strains using comparative genomics. The core genes-based tree failed to differentiate between environmental and clinical strains, while the accessory genes-based tree grouped all clinical strains together. Loss-of-function mutations were common in virulence genes related to adherence and secretion systems. Differences in pathogenicity islands and other genetic elements were observed between O145:H28 and reference strains. The study reveals the genetic diversity and evolution of virulence in STEC.
Article
Microbiology
Mathilda Fatton, Sevasti Filippidou, Thomas Junier, Guillaume Cailleau, Matthieu Berge, Daniel Poppleton, Thorsten B. Blum, Marek Kaminek, Adolfo Odriozola, Jochen Blom, Shannon L. Johnson, Jan Pieter Abrahams, Patrick S. Chain, Simonetta Gribaldo, Elitza Tocheva, Benoit Zuber, Patrick H. Viollier, Pilar Junier
Summary: This study discovered the production of spore-like structures in Kurthia strain 11kri321, which was previously considered as asporogenic. These cryptospores, however, did not offer protection against heat or UV damage and were lost after successive sub-culturing. These findings suggest that cryptosporulation might have been a prevalent ancestral developmental state in Firmicutes under harsh environmental conditions.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Rebecca D. Prescott, Tatyana Zamkovaya, Stuart P. Donachie, Diana E. Northup, Joseph J. Medley, Natalia Monsalve, Jimmy H. Saw, Alan W. Decho, Patrick S. G. Chain, Penelope J. Boston
Summary: Lava caves, tubes, and fumaroles in Hawai'i have unique bacterial communities with little overlap between different caves or sites. Older lava tubes have higher phylogenetic diversity compared to younger or geothermally active sites. Competitive exclusion is the main driver in structuring the bacterial communities in Hawaiian volcanic environments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Fabio Palmieri, Pauline Udriet, Shannon L. Johnson, Karen Davenport, Patrick S. G. Chain, Saskia Bindschedler, Pilar Junier
Summary: In this study, we sequenced and analyzed the complete genome of the soil oxalotrophic bacterium Cupriavidus oxalaticus Ox1 and a genetically modified strain with mCherry tag. The genome is approximately 6.69 Mb in size, with a GC content of 66.9%. The genome of C. oxalaticus Ox1 contains a complete operon responsible for the degradation and assimilation of oxalate.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biology
Thierry Kuhn, Matteo Buffi, Saskia Bindschedler, Patrick S. Chain, Diego Gonzalez, Claire E. Stanley, Lukas Y. Wick, Pilar Junier, Xiang-Yi Li Richter
Summary: Researchers have developed 3D printed devices that can be used as abiotic controls to study bacterial dispersal on hydrated surfaces. By separating biotic and abiotic elements, these devices will stimulate further research on microbial dispersal in soil and other water-unsaturated environments.
Article
Microbiology
Andrea Corona Ramirez, Danae Bregnard, Thomas Junier, Guillaume Cailleau, Cristina Dorador, Saskia Bindschedler, Pilar Junier
Summary: Fungi employ various strategies to form specialized structures in order to survive unfavorable environmental conditions. These structures not only disperse to other environments, but also enable fungi to survive for extended periods of time until favorable growth conditions occur. However, current methods for studying the diversity of fungal structures only target spores dispersing in the air, disregarding the high diversity of these structures in terms of morphology and environmental distribution.
Article
Mycology
Aaron Robinson, Michal Babinski, Yan Xu, Julia Kelliher, Reid Longley, Patrick Chain
Summary: Research on bacterial-fungal interactions (BFIs) has shown that fungi and bacteria often interact with each other in various ecosystems and microbiomes. However, assessing the current state of BFI research is challenging due to a lack of centralized resources. To address this issue, we have developed a publicly accessible database to document previously reported interactions between bacterial and fungal taxa, serving as a centralized resource for the field.
Article
Microbiology
Andrea Corona Ramirez, Kang Soo Lee, Adolfo Odriozola, Marek Kaminek, Roman Stocker, Benoit Zuber, Pilar Junier
Summary: The production of specialized resting cells is a survival strategy developed by organisms to withstand unfavorable environmental factors. Five bacterial taxa are recognized to form specialized resting cells, and they are characterized by low metabolic activity and higher resistance to environmental stress. A cross-species comparison of bacterial sporulation reveals a lack of common chemical signature among the specialized resting cells, with each group having a unique signature. The use of Raman spectroscopy enables the isolation of endospores based on their specific chemical signatures.
Article
Biology
Thierry Kuhn, Pilar Junier, Redouan Bshary, Celine Terrettaz, Diego Gonzalez, Xiang-Yi Li Richter
Summary: Evolutionary game theory offers models to explain competing strategies coexistence, including the rock-paper-scissors game. Escherichia coli strains, consisting of a toxin-producer, a resistant and a sensitive, have been used as a classic model for studying this game. However, previous studies often ignored the influence of ecological factors such as nutrients and toxin dynamics on the game dynamics.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Microbiology
Michelle Qiu Carter, Beatriz Quinones, Xiaohua He, Antares Pham, Diana Carychao, Michael B. Cooley, Chien-Chi Lo, Patrick S. G. Chain, Rebecca L. Lindsey, James L. Bono
Summary: Through comparative genomics and phenotypic characterization, this study assessed the pathogenic potential of Escherichia albertii strains isolated from wild birds in California. The results showed that these strains have high pathogenicity potential and could cause severe diseases in humans.
Review
Microbiology
Felix Richter, Saskia Bindschedler, Maryline Calonne-Salmon, Stephane Declerck, Pilar Junier, Claire E. Stanley
Summary: This article highlights the advancements in the field of 'Fungi-on-a-Chip' microfluidics for single-cell studies on fungi and discusses future frontiers where microfluidic technology can aid in understanding fungal biology.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Microbiology
Aislinn Estoppey, Laure Weisskopf, Eva Di Francesco, Armelle Vallat-Michel, Saskia Bindschedler, Patrick S. Chain, Pilar Junier
Summary: This study presents novel experimental platforms for in-vitro experiments with fungal spores to assess the effect of bacteria on germination and fungal growth control. The experiments demonstrated that the bacteria Cupriavidus necator and Cupriavidus oxalaticus were able to control the growth of the phytopathogenic fungus Botrytis cinerea, but only in media in which soluble oxalic acid was detected by the fungus.
FEMS MICROBIOLOGY LETTERS
(2022)
