Sediminibacterium aquarii sp nov., isolated from sediment in a fishbowl

A novel bacterial strain, designated AA5(T), was isolated from sediment in a fishbowl. Cells were Gram-stain-negative, rod-shaped and strictly aerobic, showing gliding motility. Strain AA5(T) was able to grow at 15-37 degrees C, at pH 5.0-8.0 and in the absence of NaCl. 16S rRNA gene sequence analysis revealed that strain AA5(T) was most closely related to species in the genus Sediminibacterium, showing highest similarity to the type strain of S.ediminibacterium goheungense (96.6 %). Concordantly, a phylogenetic tree based on 16S rRNA gene sequences indicated that strain AA5(T) belongs to the genus Sediminibacterium. The DNA G+C content was 44.7 mol%. The major polar lipids were phosphatidylethanolamine, four unidentified aminolipids, two unknown aminophospholipids and four unidentified polar lipids. The only respiratory quinone of strain AA5(T) was menaquinone 7 (MK-7) and the major fatty acids (>5% of the total) were iso-C-15 : 0, iso-C-15 : 1 G, iso-C-16 : 0 3-OH, iso-C-17 : 0 3-OH and iso-C-15 : 0 3-OH. Based on data from this polyphasic study, strain AA5(T) represents a novel species, for which the name Sediminibacterium aquarii sp. nov. is proposed. The type strain is AA5T (=KACC 18509(T) =JCM 31013(T)).