Actibacterium ureilyticum sp nov., isolated from seawater

A polyphasic approach was used to characterize a novel marine bacterial strain, designated LS-811(T), isolated from seawater of the South China Sea (Taiwan). Cells of strain LS-811(T) were Gram-staining negative, aerobic and rod-shaped with polar flagella. The 16S rRNA gene sequence analysis of strain LS-811(T) showed highest sequence similarity to Actibacterium mucosum (96.5 %) and Actibacterium atlanticum (95.6 %), and lower sequence similarity (<96.0 %) to members of all other related genera. Strain LS-811(T) was able to grow at 15-40 degrees C and pH 5.0-9.0. The quinone system was ubiquinone (Q-10), and the DNA G+C content was 60.1 mol%. The major fatty acids (>5 %) found in strain LS-811(T) were C-18:0, C-10:0 3-OH, C-19: cyclo omega 8c and C-18:1 omega 7c/C-18:1 omega 6c. The major polar lipid profile consisted of glycolipids, phosphatidylglycerol and one unidentified aminolipid. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain LS-811(T) is considered to represent a novel species in the genus Actibacterium, for which the name Actibacterium ureilyticum sp. nov. is proposed. The type strain is LS-811(T) (= BCRC 80823(T) = JCM 30681(T)).